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Literature summary extracted from
- Characterization of an organic solvent-tolerant thermostable glucoamylase from a halophilic isolate, Halolactibacillus sp. SK71 and its application in raw starch hydrolysis for bioethanol production (2014), Biotechnol. Prog., 30, 1262-1268.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.3 | NaCl | optimum concentration 7.5% NaCl, active at 0-20% NaCl | Halolactibacillus sp. SK71 |  |
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.2.1.3 | synthesis | enzyme may be used for raw corn starch hydrolysis and subsequent bioethanol production using Saccharomyces cerevisiae. The yield in terms of grams of ethanol produced per gram of sugar consumed is 0.365 g/g, with 71.6% of theoretical yield from raw corn starch | Halolactibacillus sp. SK71 |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.3 | extracellular | - |
Halolactibacillus sp. SK71 | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.3 | 78500 | - |
SDS-PAGE | Halolactibacillus sp. SK71 |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.3 | Halolactibacillus sp. SK71 | - |
soil isolate | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.3 | - |
Halolactibacillus sp. SK71 |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.3 | additional information | enzyme shows broad substrate specificity and raw starch hydrolyzing activity | Halolactibacillus sp. SK71 | ? | - |
? | |
| 3.2.1.3 | starch + H2O | - |
Halolactibacillus sp. SK71 | D-glucose + ? | sole end-product | ? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.3 | 70 | - |
- |
Halolactibacillus sp. SK71 |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.3 | 8 | - |
- |
Halolactibacillus sp. SK71 |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.3 | 7 | 12 | - |
Halolactibacillus sp. SK71 |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 3.2.1.3 | Halolactibacillus sp. SK71 | enzyme production is strongly influenced by the salinity of growth medium with maximum in the presence of 5% NaCl | additional information |
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Release 2023.1 (January 2023)
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