Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Dimitrijevic, A.; Velickovic, D.; Milosavic, N.; Bezbradica, D.
    Specificity of maltase to maltose in three different directions of reaction: hydrolytic, vanillyl alcohol glucoside and vanillyl alcohol isomaltoside synthesis (2012), Biotechnol. Prog., 28, 1450-1456.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.B65 Vanillyl alcohol
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.B65 3.09
-
 maltose synthesis of vanillyl alcohol alpha-D-isomaltoside, pH 6.6, 37°C Saccharomyces cerevisiae
2.4.1.B65 12.96
-
 Vanillyl alcohol pH 6.6, 37°C Saccharomyces cerevisiae
2.4.1.B65 26.24
-
 vanillyl alcohol alpha-D-glucoside pH 6.6, 37°C Saccharomyces cerevisiae
2.4.1.B65 46
-
 maltose synthesis of vanillyl alcohol alpha-glucoside, pH 6.6, 37°C Saccharomyces cerevisiae
3.2.1.20 0.27
-
 4-nitrophenyl alpha-D-glucopyranoside pH 6.6, 37°C Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.B65 63000
-
 x * 63000, SDS-PAGE Saccharomyces cerevisiae
3.2.1.20 63000
-
 x * 63000, SDS-PAGE Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.B65 Saccharomyces cerevisiae P53341
-
-
3.2.1.20 Saccharomyces cerevisiae P53341
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.B65
-
 Saccharomyces cerevisiae
3.2.1.20
-
 Saccharomyces cerevisiae

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.B65 vanillyl alcohol + maltose = 4-hydroxy-3-methoxybenzyl alpha-D-glucopyranoside + D-glucose ping-pong bi-bi mechanism Saccharomyces cerevisiae
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.20 100
-
 pH 6.6, 37°C Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.B65 additional information enzyme additionally catalyses the reaction of alpha-glucosidase/maltase, EC 3.2.1.20. The Km value of the enzyme for maltose in the synthetic reaction to vanillyl alcohol alpha-glucoside is about two times smaller than in maltose hydrolysis Saccharomyces cerevisiae ?
-
 ?
2.4.1.B65 vanillyl alcohol + maltose
-
 Saccharomyces cerevisiae vanillyl alcohol alpha-D-glucoside + D-glucose
-
 ?
2.4.1.B65 vanillyl alcohol alpha-D-glucoside + maltose
-
 Saccharomyces cerevisiae vanillyl alcohol alpha-D-isomaltoside + D-glucose at low vanillyl alcohol concentrations up to 50 mM, or if the reaction is prolonged to 3 h at higher concentrations of vanillyl alcohol, additional transglucosylation product vanillyl alcohol alpha-isomaltoside is detected. The transglucosidation takes place at the C6 hydroxyl group of vanillyl alcohol alpha-D-glucoside ?
3.2.1.20 4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Saccharomyces cerevisiae 4-nitrophenol + D-glucose
-
 ?
3.2.1.20 additional information enzyme additionally catalyses transglucosylation of vanillyl alcohol, EC 2.4.1. The reaction is very efficient with selectivity factor 149 and occurs by a ping-pong mechanism with inhibition by glucose acceptor. At low concentration of vanillyl alcohol, one additional transglucosylation product, alpha-isomaltoside of vanillyl alcohol, is detected, indicating that vanillyl alcohol glucoside is a product of the first transglucosylation reaction and a substrate for second. The rate of isomaltoside synthesis is two orders of magnitude smaller than glucoside synthesis Saccharomyces cerevisiae ?
-
 ?
3.2.1.20 additional information no substrate: isomaltose Saccharomyces cerevisiae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.B65 ? x * 63000, SDS-PAGE Saccharomyces cerevisiae
3.2.1.20 ? x * 63000, SDS-PAGE Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
2.4.1.B65 maltase
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.B65 37
-
-
 Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.B65 6.6
-
-
 Saccharomyces cerevisiae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.B65 8.94
-
 Vanillyl alcohol pH 6.6, 37°C Saccharomyces cerevisiae