EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.73 | gene plicA, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic tree, subcloning in Escherichia coli strain DH5alpha, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Paenibacillus sp. |
EC Number | General Stability | Organism |
---|---|---|
3.2.1.73 | the purified recombinant His-tagged enzyme retains over 90% activity in 4 M NaCl at 25°C for 24 h | Paenibacillus sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.73 | Cu2+ | - |
Paenibacillus sp. | |
3.2.1.73 | Fe2+ | - |
Paenibacillus sp. | |
3.2.1.73 | Fe3+ | - |
Paenibacillus sp. | |
3.2.1.73 | Mn2+ | - |
Paenibacillus sp. | |
3.2.1.73 | SDS | about 55% inhibition | Paenibacillus sp. | |
3.2.1.73 | Zn2+ | - |
Paenibacillus sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.73 | additional information | - |
additional information | 3.7 mg/ml at pH 6.2, 55°C with barley beta-glucan, recombinant enzyme | Paenibacillus sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.73 | Ca2+ | slight activtion, stabilizes the enzyme at higher temperatures | Paenibacillus sp. | |
3.2.1.73 | Co2+ | slight activtion | Paenibacillus sp. | |
3.2.1.73 | Mg2+ | slight activtion | Paenibacillus sp. | |
3.2.1.73 | additional information | EDTA has little effect on enzymatic activity | Paenibacillus sp. | |
3.2.1.73 | NaCl | 1.5fold activation at 0.5 M, the enzyme is acid-, alkali- and salt-tolerant, the purified recombinant His-tagged enzyme retains over 90% activity in 4 M NaCl at 25°C for 24 h | Paenibacillus sp. | |
3.2.1.73 | Triton X-100 | about 20% activation | Paenibacillus sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.73 | 25000 | - |
x * 239000, about, sequence calculation, x * 25000, recombinant His-tagged enzyme, SDS-PAGE | Paenibacillus sp. |
3.2.1.73 | 239000 | - |
x * 239000, about, sequence calculation, x * 25000, recombinant His-tagged enzyme, SDS-PAGE | Paenibacillus sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.73 | additional information | Paenibacillus sp. | the enzyme specifically catalyzes the hydrolysis of beta-1,4-glycosidic bonds located prior to beta-1,3-glycosidic linkages in 1,3-1,4-beta-glucan | ? | - |
? | |
3.2.1.73 | additional information | Paenibacillus sp. S09 | the enzyme specifically catalyzes the hydrolysis of beta-1,4-glycosidic bonds located prior to beta-1,3-glycosidic linkages in 1,3-1,4-beta-glucan | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.73 | Paenibacillus sp. | M9XGK2 | gene plicA | - |
3.2.1.73 | Paenibacillus sp. S09 | M9XGK2 | gene plicA | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.73 | recombinant His-tagged enzyme 2.5fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Paenibacillus sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.73 | 7055 | - |
purified recombinant His-tagged enzyme, substrate barley beta-glucan, pH 6.2, 55°C | Paenibacillus sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.73 | barley beta-glucan + H2O | - |
Paenibacillus sp. | ? | - |
? | |
3.2.1.73 | barley beta-glucan + H2O | - |
Paenibacillus sp. S09 | ? | - |
? | |
3.2.1.73 | additional information | the enzyme specifically catalyzes the hydrolysis of beta-1,4-glycosidic bonds located prior to beta-1,3-glycosidic linkages in 1,3-1,4-beta-glucan | Paenibacillus sp. | ? | - |
? | |
3.2.1.73 | additional information | enzyme substrate specificity, overview. No activity with laminarin, carboxymethyl cellulose, xylan and soluble starch | Paenibacillus sp. | ? | - |
? | |
3.2.1.73 | additional information | the enzyme specifically catalyzes the hydrolysis of beta-1,4-glycosidic bonds located prior to beta-1,3-glycosidic linkages in 1,3-1,4-beta-glucan | Paenibacillus sp. S09 | ? | - |
? | |
3.2.1.73 | additional information | enzyme substrate specificity, overview. No activity with laminarin, carboxymethyl cellulose, xylan and soluble starch | Paenibacillus sp. S09 | ? | - |
? | |
3.2.1.73 | oat beta-glucan + H2O | - |
Paenibacillus sp. | ? | - |
? | |
3.2.1.73 | oat beta-glucan + H2O | - |
Paenibacillus sp. S09 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.73 | ? | x * 239000, about, sequence calculation, x * 25000, recombinant His-tagged enzyme, SDS-PAGE | Paenibacillus sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.73 | beta-1,3-1,4-glucanase | - |
Paenibacillus sp. |
3.2.1.73 | Lichenase | - |
Paenibacillus sp. |
3.2.1.73 | PlicA | - |
Paenibacillus sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.73 | 55 | - |
- |
Paenibacillus sp. |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.73 | 30 | 70 | recombinant His-tagged enzyme, activity range, profile, overview | Paenibacillus sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.73 | 50 | - |
purified recombinant His-tagged enzyme retaining more than 70% activity at up to 50°C for 2 h | Paenibacillus sp. |
3.2.1.73 | 55 | - |
purified recombinant His-tagged enzyme, half-life is about 50 min, 10% activity remaining after 2 h | Paenibacillus sp. |
3.2.1.73 | 60 | - |
purified recombinant His-tagged enzyme, inactivation within 10 min, in presence of 5 mM CaCl2 the enzyme retains over 80% activity after 2 h | Paenibacillus sp. |
3.2.1.73 | 65 | - |
purified recombinant His-tagged enzyme, in presence of 5 mM CaCl2 the enzyme retains 40% activity after 10 min, and 20% after 20 min, inactivation after 1 h | Paenibacillus sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.73 | 6.2 | - |
- |
Paenibacillus sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.73 | 3.5 | 10 | recombinant His-tagged enzyme, activity range, profile, overview | Paenibacillus sp. |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.73 | 3.5 | 12 | the enzyme is acid-, alkali- and salt-tolerant, the purified recombinant His-tagged enzyme retains more than 80% activity after incubation for 4 h | Paenibacillus sp. |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.2.1.73 | evolution | the enzyme is a member of the glycosylhydrolase family GH16 containing the conserved motif EIDIEF and signal peptide | Paenibacillus sp. |