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Literature summary extracted from

  • Shi, H.; Zhang, Y.; Zhong, H.; Huang, Y.; Li, X.; Wang, F.
    Cloning, over-expression and characterization of a thermo-tolerant xylanase from Thermotoga thermarum (2014), Biotechnol. Lett., 36, 587-593.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 expression in Escherichia coli Pseudothermotoga thermarum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.8 homology modeling of structure. Residues Glu145 and Glu252 are the catalytic nucleophile and proton donor, respectively Pseudothermotoga thermarum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 Al3+ 1 mM, 46% residual activity Pseudothermotoga thermarum
3.2.1.8 Ba2+ 1 mM, 72% residual activity Pseudothermotoga thermarum
3.2.1.8 Cu2+ 1 mM, no residual activity Pseudothermotoga thermarum
3.2.1.8 Mg2+ 1 mM, 46% residual activity Pseudothermotoga thermarum
3.2.1.8 sodium dodecylsulfate 0.1%, 82% residual activity Pseudothermotoga thermarum
3.2.1.8 Tween 60 0.05%, 78% residual activity Pseudothermotoga thermarum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.8 additional information
-
 beechwood xylan Km value is 1.8 mg/ml, pH 6.0, 80°C Pseudothermotoga thermarum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.8 Mn2+ 1 mM, 133% of initial activity Pseudothermotoga thermarum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 40000
-
 x * 40531, calculated, x * 40000, SDS-PAGE Pseudothermotoga thermarum
3.2.1.8 40531
-
 x * 40531, calculated, x * 40000, SDS-PAGE Pseudothermotoga thermarum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Pseudothermotoga thermarum F7YXD6
-
-
3.2.1.8 Pseudothermotoga thermarum DSM 5069 F7YXD6
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 beechwood xylan + H2O
-
 Pseudothermotoga thermarum ?
-
 ?
3.2.1.8 beechwood xylan + H2O
-
 Pseudothermotoga thermarum DSM 5069 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 ? x * 40531, calculated, x * 40000, SDS-PAGE Pseudothermotoga thermarum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 Xyn10B
-
 Pseudothermotoga thermarum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 80
-
-
 Pseudothermotoga thermarum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 75
-
 2 h, 75% resiudal activity Pseudothermotoga thermarum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.8 520
-
 beechwood xylan pH 6.0, 80°C Pseudothermotoga thermarum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 6
-
-
 Pseudothermotoga thermarum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.8 5.5 7.5 50°C, stable for 1 h Pseudothermotoga thermarum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.8 Pseudothermotoga thermarum calculated
-
 6.4

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.8 additional information
-
 beechwood xylan kcat/Km value is 289 ml per mg and s, pH 6.0, 80°C Pseudothermotoga thermarum