Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kumari, A.; Gupta, R.
    Extracellular expression and characterization of thermostable lipases, LIP8, LIP14 and LIP18, from Yarrowia lipolytica (2012), Biotechnol. Lett., 34, 1733-1739.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.3 beta-mercaptoethanol 1.8fold enhancement of activity at 90% (v/v) Yarrowia lipolytica
3.1.1.3 beta-mercaptoethanol 1.9fold enhancement of activity at 90% (v/v) Yarrowia lipolytica
3.1.1.3 dithiothreitol 1.4fold enhancement of activity at 90% (w/v) Yarrowia lipolytica
3.1.1.3 dithiothreitol 1.5fold enhancement of activity at 90% (w/v) Yarrowia lipolytica
3.1.1.3 dithiothreitol 1.7fold enhancement of activity at 90% (w/v) Yarrowia lipolytica

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.3 expressed in Escherichia coli HB101 pEZZ18 cells Yarrowia lipolytica

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 1,10-phenanthroline
-
 Yarrowia lipolytica
3.1.1.3 Cd2+
-
 Yarrowia lipolytica
3.1.1.3 DTNB
-
 Yarrowia lipolytica
3.1.1.3 Fe2+
-
 Yarrowia lipolytica
3.1.1.3 N-bromosuccinamide
-
 Yarrowia lipolytica
3.1.1.3 Ni2+
-
 Yarrowia lipolytica
3.1.1.3 phenylmethylsulfonyl fluoride
-
 Yarrowia lipolytica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.3 Ca2+ the enzyme is activated by 6 mM Ca2+ Yarrowia lipolytica
3.1.1.3 Mg2+ does not affect the enzyme activity Yarrowia lipolytica

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Yarrowia lipolytica I0CKV2
-
-
3.1.1.3 Yarrowia lipolytica I0CKV3
-
-
3.1.1.3 Yarrowia lipolytica Q3HRV6
-
-
3.1.1.3 Yarrowia lipolytica MSR80 I0CKV2
-
-
3.1.1.3 Yarrowia lipolytica MSR80 I0CKV3
-
-
3.1.1.3 Yarrowia lipolytica MSR80 Q3HRV6
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 4-nitrophenyl caproate + H2O low activity Yarrowia lipolytica 4-nitrophenol + caproate
-
 ?
3.1.1.3 4-nitrophenyl caproate + H2O low activity Yarrowia lipolytica MSR80 4-nitrophenol + caproate
-
 ?
3.1.1.3 4-nitrophenyl palmitate + H2O high activity Yarrowia lipolytica 4-nitrophenol + palmitate
-
 ?
3.1.1.3 4-nitrophenyl palmitate + H2O high activity Yarrowia lipolytica MSR80 4-nitrophenol + palmitate
-
 ?
3.1.1.3 amla oil + H2O
-
 Yarrowia lipolytica ?
-
 ?
3.1.1.3 cotton seed oil + H2O
-
 Yarrowia lipolytica ?
-
 ?
3.1.1.3 cotton seed oil + H2O
-
 Yarrowia lipolytica MSR80 ?
-
 ?
3.1.1.3 groundnut oil + H2O
-
 Yarrowia lipolytica ?
-
 ?
3.1.1.3 groundnut oil + H2O
-
 Yarrowia lipolytica MSR80 ?
-
 ?
3.1.1.3 tridecanoate + H2O
-
 Yarrowia lipolytica ?
-
 ?
3.1.1.3 trilaurin + H2O
-
 Yarrowia lipolytica ?
-
 ?
3.1.1.3 trimyristin + H2O
-
 Yarrowia lipolytica ?
-
 ?
3.1.1.3 triolein + H2O
-
 Yarrowia lipolytica ?
-
 ?
3.1.1.3 triolein + H2O
-
 Yarrowia lipolytica MSR80 ?
-
 ?
3.1.1.3 tripalmitin + H2O
-
 Yarrowia lipolytica ?
-
 ?
3.1.1.3 tristearin + H2O
-
 Yarrowia lipolytica ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 LIP14
-
 Yarrowia lipolytica
3.1.1.3 LIP18
-
 Yarrowia lipolytica
3.1.1.3 LIP8
-
 Yarrowia lipolytica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 40
-
-
 Yarrowia lipolytica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 40 80 the enzyme is thermostable with half-life of 49 min at 80°C Yarrowia lipolytica
3.1.1.3 40 80 the enzyme is thermostable with half-life of 52 min at 80°C Yarrowia lipolytica
3.1.1.3 40 80 the enzyme is thermostable with half-life of 68 min at 80°C Yarrowia lipolytica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 7
-
-
 Yarrowia lipolytica