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Literature summary extracted from

  • Badhan, A.; Jin, L.; Wang, Y.; Han, S.; Kowalczys, K.; Brown, D.C.; Ayala, C.J.; Latoszek-Green, M.; Miki, B.; Tsang, A.; McAllister, T.
    Expression of a fungal ferulic acid esterase in alfalfa modifies cell wall digestibility (2014), Biotechnol. Biofuels, 7, 39.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.73 gene faeB, subcloned into pEACH vector without its own N-terminal signal peptide coding region, but containing different signaling peptides to target the enzyme to the apoplast, chloroplast, endoplasmic reticulum, and vacuole. Four constructs harboring faeB are transiently expressed in Nicotiana tabacum leaves, with the enzyme accumulating at high levels in all target sites, except chloroplast. Stable expression in Medicago sativa using Agrobacterium tumefaciens strain LBA4404 transformation method Aspergillus niger

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.73 additional information transgenic alflafa plants expressing the enzyme possess modified cell wall morphology and composition with a reduction in ester linkages and elevated lignin content, they are more recalcitrant to digestion by mixed ruminal microorganisms, phenotypes, detailed overview Aspergillus niger

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.73 extracellular 18 amino acids in the Nterminus function as a signal peptide that aides in the secretion of the enzyme Aspergillus niger
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Aspergillus niger Q8WZI8
-
-
3.1.1.73 Aspergillus niger Q8WZI8 gene faeB
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 ethyl ferulate + H2O
-
 Aspergillus niger ethanol + ferulate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 FAEB
-
 Aspergillus niger
3.1.1.73 ferulic acid esterase
-
 Aspergillus niger
3.1.1.73 feruloyl esterase B
-
 Aspergillus niger
3.1.1.73 type B ferulic acid esterase
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 30
-
 assay at Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 7.2
-
 assay at Aspergillus niger

General Information

EC Number General Information Comment Organism
3.1.1.73 physiological function expression in Medicago sativa and Nicotiana tabacum using different signaling peptides to target type B ferulic acid esterase proteins to the apoplast, chloroplast, endoplasmic reticulum and vacuole. In Nicotiana leaves, the protein accumulates at high levels in all target sites, except chloroplast. Stable transformed lines of alfalfa possess modified cell wall morphology and composition with a reduction in ester linkages and elevated lignin content. These are more recalcitrant to digestion by mixed ruminal microorganisms. Delignification by alkaline peroxide treatment followed by exposure to a commercial cellulase mixture results in higher glucose release from transgenic lines as compared to the control line Aspergillus niger