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Literature summary extracted from

  • Anand, A.; Srivastava, P.K.
    Isolation and enzymatic properties of a nonspecific acid phosphatase from Vigna aconitifolia seeds (2014), Biotechnol. Appl. Biochem., 61, 145-152.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 Cu2+
-
 Vigna aconitifolia
3.1.3.2 F-
-
 Vigna aconitifolia
3.1.3.2 Fe3+
-
 Vigna aconitifolia
3.1.3.2 Mo6+
-
 Vigna aconitifolia
3.1.3.2 phosphate
-
 Vigna aconitifolia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 0.1
-
 5'-AMP pH 5.0 30°C Vigna aconitifolia
3.1.3.2 0.11
-
 5'-ADP pH 5.0 30°C Vigna aconitifolia
3.1.3.2 0.13
-
 2-naphthyl phosphate pH 5.0 30°C Vigna aconitifolia
3.1.3.2 0.15
-
 4-nitrophenyl phosphate pH 5.0 30°C Vigna aconitifolia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.2 24000
-
 1 * 24000 plus 1 * 27000, SDS-PAGE Vigna aconitifolia
3.1.3.2 27000
-
 1 * 24000 plus 1 * 27000, SDS-PAGE Vigna aconitifolia
3.1.3.2 58000
-
 gel filtration Vigna aconitifolia

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Vigna aconitifolia
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2
-
 Vigna aconitifolia

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.2 seed
-
 Vigna aconitifolia
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.2 29.6
-
 pH 5.0, 30°C Vigna aconitifolia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 2-naphthyl phosphate + H2O 59% of the activity with 4-nitrophenyl phosphate Vigna aconitifolia 2-naphthol + phosphate
-
 ?
3.1.3.2 4-nitrophenyl phosphate + H2O
-
 Vigna aconitifolia 4-nitrophenol + phosphate
-
 ?
3.1.3.2 5'-ADP + H2O 24% of the activity with 4-nitrophenyl phosphate Vigna aconitifolia 5'-AMP + phosphate
-
 ?
3.1.3.2 5'-AMP + H2O 38% of the activity with 4-nitrophenyl phosphate Vigna aconitifolia adenosine + phosphate
-
 ?
3.1.3.2 additional information enzyme additionally cleaves glucose 6-phosphate and fructose 6-phosphate with similar activity as AMP Vigna aconitifolia ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.2 dimer 1 * 24000 plus 1 * 27000, SDS-PAGE Vigna aconitifolia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.2 60
-
-
 Vigna aconitifolia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 5
-
-
 Vigna aconitifolia

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.2 4.6 6
-
 Vigna aconitifolia