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Literature summary extracted from

  • Jiang, H.X.; Kong, D.M.; Shen, H.X.
    Amplified detection of DNA ligase and polynucleotide kinase/phosphatase on the basis of enrichment of catalytic G-quadruplex DNAzyme by rolling circle amplification (2014), Biosens. Bioelectron., 55, 133-138.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.32 3'-phospho-5'-hydroxy-DNA + H2O Tequatrovirus T4
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Organism

EC Number Organism UniProt Comment Textmining
3.1.3.32 Tequatrovirus T4
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.32 3'-phospho-5'-hydroxy-ATTCGTGTGAGAAAACCCAACCCGCCCTACCCAAAAGTCAGATGA + H2O the enzyme mediates 5'-phosphorylation of ATTCGTGTGAGAAAACCCAACCCGCCCTACCCAAAAGTCAGATGA Tequatrovirus T4 ?
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3.1.3.32 3'-phospho-5'-hydroxy-DNA + H2O
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 Tequatrovirus T4 ?
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 ?
3.1.3.32 additional information polynucleotide kinase/phosphatase is a bifunctional enzyme with 5'-kinase and 3'-phosphatase activities which can generate 5'-phosphate and 3'-hydroxyl groups at damaged DNA termini, thus permitting subsequent repair proteins to replace missing nucleotides and rejoin broken strands Tequatrovirus T4 ?
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Synonyms

EC Number Synonyms Comment Organism
3.1.3.32 PNKP
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 Tequatrovirus T4
3.1.3.32 polynucleotide kinase/phosphatase
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 Tequatrovirus T4

General Information

EC Number General Information Comment Organism
3.1.3.32 physiological function this enzyme plays an important role in nucleic acid metabolism and DNA repairing during strand interruption Tequatrovirus T4