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Literature summary extracted from

  • He, Y.; Xiong, L.H.; Xing, X.J.; Tang, H.W.; Pang, D.W.
    An ultra-high sensitive platform for fluorescence detection of micrococcal nuclease based on graphene oxide (2013), Biosens. Bioelectron., 42, 467-473.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.31.1 analysis the existence of MNase can be the standard to identify Staphylococcus aureus, and the content of MNase can be used to evaluate the pathogenicity of Staphylococcus aureus, usage of a graphene oxide-based biosensor, method development, overview Staphylococcus aureus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.31.1 extracellular
-
 Staphylococcus aureus
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.31.1 Staphylococcus aureus
-
-
-
3.1.31.1 Staphylococcus aureus CCTCC AB91093
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.31.1 additional information method development for an ultra-high sensitive and selective fluorescent sensing platform for the enzyme based on enzyme-induced DNA strand scission and the difference in affinity of graphene oxide for single-stranded DNA containing different numbers of bases in length, overview. The adsorption of the dye-labeled ssDNA on graphene oxide makes the dyes close proximity to graphene oxide surface resulting in high efficiency quenching of fluorescence of the dyes. Conversely, and very importantly, in the presence of MNase, it cleaves the dye-labeled ssDNA into small fragments. Substrates are commercial and 6-carboxyfluorescein (FAM)-labeled: 20-mer ssDNA with a sequence of 5'-FAM-TATATGGATGATGTGGTATT-3', 10-mer ssDNA with a sequence of 5'FAM-TATATGGATG-3', and 5-mer ssDNA with a sequence of 5'FAM-TATAT-3' Staphylococcus aureus ?
-
 ?
3.1.31.1 additional information method development for an ultra-high sensitive and selective fluorescent sensing platform for the enzyme based on enzyme-induced DNA strand scission and the difference in affinity of graphene oxide for single-stranded DNA containing different numbers of bases in length, overview. The adsorption of the dye-labeled ssDNA on graphene oxide makes the dyes close proximity to graphene oxide surface resulting in high efficiency quenching of fluorescence of the dyes. Conversely, and very importantly, in the presence of MNase, it cleaves the dye-labeled ssDNA into small fragments. Substrates are commercial and 6-carboxyfluorescein (FAM)-labeled: 20-mer ssDNA with a sequence of 5'-FAM-TATATGGATGATGTGGTATT-3', 10-mer ssDNA with a sequence of 5'FAM-TATATGGATG-3', and 5-mer ssDNA with a sequence of 5'FAM-TATAT-3' Staphylococcus aureus CCTCC AB91093 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.31.1 MNase
-
 Staphylococcus aureus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.31.1 37
-
 assay at Staphylococcus aureus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.31.1 8
-
 assay at Staphylococcus aureus