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Literature summary extracted from

  • Akond, M.A.; Matsuda, Y.; Ishimaru, T.; Iwai, K.; Saito, A.; Kato, A.; Tanaka, S.; Kobayashi, J.; Koga, D.
    Characterization of a yam class IV chitinase produced by recombinant Pichia pastoris X-33 (2014), Biosci. Biotechnol. Biochem., 78, 222-230.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 cloning in Escherichia coli strain JM109, removal of the C-terminal VTS of 24 bp and replacement of the N-terminal signal sequence of yam chitinase with alpha-factor of the expression vector pPICZalphaA, recombinant expression in Pichia pastoris strain X-33 an secretion to the culture medium Dioscorea polystachya

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.14 additional information
-
 additional information 0.233 mg/ml at pH 8.0, 32°C, substrate glycol chitin, recombinant enzyme Dioscorea polystachya

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.14 extracellular
-
 Dioscorea polystachya
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 32000
-
 x * 32000, recombinant deglycosylated enzyme, SDS-PAGE Dioscorea polystachya

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.14 chitin + H2O Dioscorea polystachya
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Dioscorea polystachya Q852P9
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.14 glycoprotein deglycosylation by endoglycosidase H Dioscorea polystachya

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 recombinant extracellular enzyme 10fold from Pichia pastoris culture supernatant by several different steps of anion exchange chromatography and gel filtration Dioscorea polystachya

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 6.23
-
 purified recombinant enzyme, pH 8.0, 32°C, substrate glycolchitin Dioscorea polystachya

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 chitin + H2O
-
 Dioscorea polystachya ?
-
 ?
3.2.1.14 glycol chitin + H2O
-
 Dioscorea polystachya ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 32000, recombinant deglycosylated enzyme, SDS-PAGE Dioscorea polystachya

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 class IV chitinase
-
 Dioscorea polystachya

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 70
-
-
 Dioscorea polystachya

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.14 40
-
 purified native deglycosylated enzyme, pH 8.0, 15 min, completely stable Dioscorea polystachya
3.2.1.14 60
-
 purified native deglycosylated enzyme, pH 8.0, 15 min, over 90% activity remaining Dioscorea polystachya
3.2.1.14 70
-
 purified native deglycosylated enzyme, pH 8.0, 15 min, over 60% activity remaining Dioscorea polystachya
3.2.1.14 75
-
 purified native deglycosylated enzyme, pH 8.0, 15 min, 10% activity remaining Dioscorea polystachya

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.14 0.2
-
 glycol chitin pH 8.0, 32°C, recombinant enzyme Dioscorea polystachya

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 5
-
 main larger peak Dioscorea polystachya
3.2.1.14 8
-
 second smaller peak Dioscorea polystachya

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.14 3 12 activity range, profile overview Dioscorea polystachya

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.14 3 12 purified native deglycosylated enzyme, 32°C, completely stable Dioscorea polystachya

General Information

EC Number General Information Comment Organism
3.2.1.14 evolution the enzyme belongs to the glycosyl hydrolase family GH 19 Dioscorea polystachya
3.2.1.14 physiological function recombinant Pichia pastoris-produced chitinase shows strong lytic activity against Fusarium oxysporum and Phytophthora nicotianae Dioscorea polystachya