Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kotani, A.; Tsuji, M.; Azama, Y.; Ishii, T.; Takeda, T.; Yamashita, T.; Shimojima, M.; Konishi, T.
    Purification and characterization of UDP-arabinopyranose mutase from Chlamydomonas reinhardtii (2013), Biosci. Biotechnol. Biochem., 77, 1874-1878.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.30 expressed in Escherichia coli Chlamydomonas reinhardtii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.99.30 0.148
-
 UDP-beta-L-arabinopyranose pH 6, 25°C, native enzyme Chlamydomonas reinhardtii
5.4.99.30 0.152
-
 UDP-beta-L-arabinofuranose pH 6, 25°C, native enzyme Chlamydomonas reinhardtii
5.4.99.30 0.371
-
 UDP-beta-L-arabinofuranose pH 6, 25°C, recombinant enzyme Chlamydomonas reinhardtii
5.4.99.30 10.3
-
 UDP-beta-L-arabinopyranose pH 6, 25°C, recombinant enzyme Chlamydomonas reinhardtii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.4.99.30 cytosol
-
 Chlamydomonas reinhardtii 5829
-

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.30 Chlamydomonas reinhardtii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.99.30 purified 94fold using hydrophobic chromatography and gel filtration Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.30 UDP-beta-L-arabinofuranose
-
 Chlamydomonas reinhardtii UDP-beta-L-arabinopyranose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.4.99.30 CrUAM
-
 Chlamydomonas reinhardtii
5.4.99.30 UDP-arabinopyranose mutase
-
 Chlamydomonas reinhardtii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.4.99.30 65
-
-
 Chlamydomonas reinhardtii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.4.99.30 60
-
 enzymatic activity decreases to 24.5% after 60 min Chlamydomonas reinhardtii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.4.99.30 0.93
-
 UDP-beta-L-arabinopyranose pH 6, 25°C, recombinant enzyme Chlamydomonas reinhardtii
5.4.99.30 1.44
-
 UDP-beta-L-arabinopyranose pH 6, 25°C, native enzyme Chlamydomonas reinhardtii
5.4.99.30 1.44
-
 UDP-beta-L-arabinofuranose pH 6, 25°C, recombinant enzyme Chlamydomonas reinhardtii
5.4.99.30 5.98
-
 UDP-beta-L-arabinofuranose pH 6, 25°C, native enzyme Chlamydomonas reinhardtii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.99.30 6
-
-
 Chlamydomonas reinhardtii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.4.99.30 5 6
-
 Chlamydomonas reinhardtii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.4.99.30 0.09
-
 UDP-beta-L-arabinopyranose pH 6, 25°C, recombinant enzyme Chlamydomonas reinhardtii
5.4.99.30 0.7
-
 UDP-beta-L-arabinofuranose pH 6, 25°C, recombinant enzyme Chlamydomonas reinhardtii
5.4.99.30 9.73
-
 UDP-beta-L-arabinopyranose pH 6, 25°C, native enzyme Chlamydomonas reinhardtii
5.4.99.30 39.3
-
 UDP-beta-L-arabinofuranose pH 6, 25°C, native enzyme Chlamydomonas reinhardtii