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Literature summary extracted from

  • Itagaki, S.; Haga, M.; Oikawa, Y.; Sakoda, A.; Ohke, Y.; Sawada, H.; Eguchi, T.; Tamegai, H.
    Differences in the roles of a glutamine amidotransferase subunit of pyridoxal 5'-phosphate synthase between Bacillus circulans and Bacillus subtilis (2013), Biosci. Biotechnol. Biochem., 77, 1481-1485.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.3.6 gene btrC2, DNA and amino acid sequence determination, genetic organization, gene btrC2 is not found in the butirosin-biosynthestic gene cluster, sequence comparison, gene complementation of a btrC2 disruptant by Bacillus subtilis gene pdxT, recombinant expression of untagged BtrC2 in Escherichia coli Niallia circulans
4.3.3.6 gene pdxS, DNA and amino acid sequence determination, genetic organization, recombinant expression of untagged protein encoded by pdxS in Escherichia coli Niallia circulans
4.3.3.6 genes pdxS and pdxT, genetic organization, expression of His-tagged PdxT in Escherichia coli Bacillus subtilis

Protein Variants

EC Number Protein Variants Comment Organism
4.3.3.6 additional information generation of a gene btrC2 disruptant mutant strain 4-41, which shows deficiency in growth and antibiotic production, and the addition of pyridoxal to the medium restores growth and short-term antibiotics production Niallia circulans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.3.6 additional information Niallia circulans BtrC2 shows no catalytic activity as 2-deoxy-scyllo-inosose (DOI) synthase ?
-
 ?
4.3.3.6 additional information Bacillus subtilis the enzyme catalyzes the conversion of ribose 5-phosphate and glyceraldehyde 3-phosphate to pyridoxal 5'-phosphate ?
-
 ?
4.3.3.6 additional information Niallia circulans SANK 72073 BtrC2 shows no catalytic activity as 2-deoxy-scyllo-inosose (DOI) synthase ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.3.6 Bacillus subtilis
-
 genes pdxS and pdxT
-
4.3.3.6 Niallia circulans Q8L1A7 btrC2; gene btrC2
-
4.3.3.6 Niallia circulans Q8L1A8 pdxS; gene pdxS
-
4.3.3.6 Niallia circulans SANK 72073 Q8L1A7 btrC2; gene btrC2
-
4.3.3.6 Niallia circulans SANK 72073 Q8L1A8 pdxS; gene pdxS
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.3.6 additional information BtrC2 shows no catalytic activity as 2-deoxy-scyllo-inosose (DOI) synthase Niallia circulans ?
-
 ?
4.3.3.6 additional information the enzyme catalyzes the conversion of ribose 5-phosphate and glyceraldehyde 3-phosphate to pyridoxal 5'-phosphate Bacillus subtilis ?
-
 ?
4.3.3.6 additional information BtrC2 shows no catalytic activity as 2-deoxy-scyllo-inosose (DOI) synthase Niallia circulans SANK 72073 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.3.3.6 PLP synthase
-
 Bacillus subtilis
4.3.3.6 PLP synthase
-
 Niallia circulans
4.3.3.6 pyridoxal 5'-phosphate synthase
-
 Bacillus subtilis
4.3.3.6 pyridoxal 5'-phosphate synthase
-
 Niallia circulans

General Information

EC Number General Information Comment Organism
4.3.3.6 malfunction a btrC2 disruptant shows deficiency in growth and antibiotic production, and the addition of pyridoxal to the medium restores growth and short-term antibiotics production Niallia circulans
4.3.3.6 additional information proteins PdxS and PdxT interact physically and form the PLP synthase, PdxT is a glutamine amidotransferase subunit that donates an amido group from glutamine to the intermediate of the enzyme reaction Bacillus subtilis