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Literature summary extracted from

  • Laribi-Habchi, H.; Dziril, M.; Badis, A.; Mouhoub, S.; Mameri, N.
    Purification and characterization of a highly thermostable chitinase from the stomach of the red scorpionfish Scorpaena scrofa with bioinsecticidal activity toward cowpea weevil Callosobruchus maculatus (Coleoptera: Bruchidae) (2012), Biosci. Biotechnol. Biochem., 76, 1733-1740.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.14 agriculture the enzyme might be used in biotechnological strategies of insect management for stored cowpeas Scorpaena scrofa

General Stability

EC Number General Stability Organism
3.2.1.14 Co2+ stabilizes the purified enzyme Scorpaena scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.14 4-chloromercuribenzoate complete inhibition Scorpaena scrofa
3.2.1.14 Hg+ complete inhibition Scorpaena scrofa
3.2.1.14 Hg2+ complete inhibition Scorpaena scrofa
3.2.1.14 N-ethylmaleimide complete inhibition Scorpaena scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.14 additional information
-
 additional information Michaelis-Menten kinetics Scorpaena scrofa
3.2.1.14 0.419
-
 colloidal chitin pH 5.0, 37°C Scorpaena scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.14 extracellular
-
 Scorpaena scrofa
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.14 Co2+ highl activating at 2 mM, Co2+ stabilizes the purified enzyme Scorpaena scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 50000
-
 gel filtration Scorpaena scrofa
3.2.1.14 50103
-
 1 * 50103, mass spectrometry, 1 * 50000, SDS-PAGE Scorpaena scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.14 chitin + H2O Scorpaena scrofa
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Scorpaena scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 native extracellular enzyme 162.75fold from stomach to homogeneity by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography Scorpaena scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.14 stomach
-
 Scorpaena scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 6.51
-
 purified enzyme, pH 5.0, 37°C, substrate colloidal chitin Scorpaena scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 4-nitrophenyl beta-N,N',N'',N'''-tetraacetylchitotetraoside + 3 H2O
-
 Scorpaena scrofa 4-nitrophenol + 2 N,N'-diacetylchitobiose
-
 ?
3.2.1.14 4-nitrophenyl beta-N,N',N''-triacetylchitotrioside + H2O
-
 Scorpaena scrofa ?
-
 ?
3.2.1.14 4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O
-
 Scorpaena scrofa 4-nitrophenol + N,N'-diacetylchitobiose
-
 ?
3.2.1.14 chitin + H2O
-
 Scorpaena scrofa ?
-
 ?
3.2.1.14 chitotriose + H2O
-
 Scorpaena scrofa ?
-
 ?
3.2.1.14 colloidal chitin + H2O
-
 Scorpaena scrofa ?
-
 ?
3.2.1.14 glycol chitin + H2O
-
 Scorpaena scrofa ?
-
 ?
3.2.1.14 glycol chitosan + H2O
-
 Scorpaena scrofa ?
-
 ?
3.2.1.14 additional information the enzyme hydrolyzes randomly beta-14,-N-acetylglucosamine linkages in the chitin polymer, and preferentially hydrolyzes the second glycosidic link from the nonreducing end of the N-acetylglucosamine oligomers Scorpaena scrofa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 monomer 1 * 50103, mass spectrometry, 1 * 50000, SDS-PAGE Scorpaena scrofa

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 SsChi50
-
 Scorpaena scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 80
-
-
 Scorpaena scrofa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.14 40 100 over 50% activity in this range, profile overview Scorpaena scrofa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.14 70
-
 purified enzyme, pH 5.0, with 2 mM Co2+: completely stable for 10 h, half-life is 18 h, about 10% activity remaining after 24 h, without Co2+: completely stable for 6 h, half-life is 14 h, inactivation after 20 h Scorpaena scrofa
3.2.1.14 80
-
 purified enzyme, pH 5.0, with 2 mM Co2+: completely stable for 4 h, half-life is 12 h, inactivation after 16 h, without Co2+: completely stable for 2 h, half-life is 10 h, inactivation after 18 h Scorpaena scrofa
3.2.1.14 90
-
 purified enzyme, pH 5.0, with 2 mM Co2+: completely stable for 2 h, half-life is 8 h, inactivation after 12 h, without Co2+: half-life is 6 h, inactivation after 14 h Scorpaena scrofa
3.2.1.14 100
-
 purified enzyme, pH 5.0, with 2 mM Co2+: half-life is 4 h, inactivation after 6 h, without Co2+: half-life is 2 h, inactivation after 8 h Scorpaena scrofa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.14 5.33
-
 colloidal chitin pH 5.0, 37°C Scorpaena scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 5
-
-
 Scorpaena scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.14 2 11 activity range, profile overview Scorpaena scrofa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.14 2
-
 purified enzyme, 60°C, 48 h, loss of 25% activity Scorpaena scrofa
3.2.1.14 3 7 purified enzyme, 60°C, 48 h, completely stable at Scorpaena scrofa
3.2.1.14 8
-
 purified enzyme, 60°C, 48 h, loss of 20% activity Scorpaena scrofa
3.2.1.14 9
-
 purified enzyme, 60°C, 48 h, loss of 30% activity Scorpaena scrofa

General Information

EC Number General Information Comment Organism
3.2.1.14 evolution the enzyme belongs to the glycosyl hydrolase family GH18 Scorpaena scrofa
3.2.1.14 physiological function the chitinase from the stomach of the red scorpionfish Scorpaena scrofa has bioinsecticidal activity toward cowpea weevil Callosobruchus maculatus Scorpaena scrofa