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Literature summary extracted from

  • Debeire, P.; Khoune, P.; Jeltsch, J.M.; Phalip, V.
    Product patterns of a feruloyl esterase from Aspergillus nidulans on large feruloyl-arabino-xylo-oligosaccharides from wheat bran (2012), Biores. Technol., 119, 425-428.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.73 additional information the feruloyl esterase from Aspergillus nidulans can be useful for the enzymatic deconstruction of xylans in plant cell walls Aspergillus nidulans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.73 expression of His-tagged enzyme in Pichia pastoris Aspergillus nidulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.73 0.23 0.32 feruloyl arabino-xylo-oligosaccharides recombinant His-tagged enzyme, pH 7.0, 37°C Aspergillus nidulans
3.1.1.73 0.96
-
 methyl ferulate recombinant His-tagged enzyme, pH 7.0, 37°C Aspergillus nidulans

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Aspergillus nidulans Q5B2G3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.73 recombinant His-tagged enzyme from Pichia pastoris by ammonium sulfate fractionation and metal chelating affinity chromatography, to homogeneity Aspergillus nidulans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.73 20.3 25.4 substrate feruloyl arabino-xylo-oligosaccharides, recombinant His-tagged enzyme, pH 7.0, 37°C Aspergillus nidulans
3.1.1.73 21.7
-
 substrate methyl ferulate, recombinant His-tagged enzyme, pH 7.0, 37°C Aspergillus nidulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 feruloyl arabino-xylo-oligosaccharides + H2O from wheat bran Aspergillus nidulans ? product pattern, overview ?
3.1.1.73 methyl ferulate + H2O
-
 Aspergillus nidulans methanol + ferulate
-
 ?
3.1.1.73 additional information the feruloyl esterase shows similar specific activity but an affinity about 3.5fold higher towards feruloyl oligosaccharides than towards methyl ferulate. The esterase is able to hydrolyze the largest feruloyl oligosaccharides and therefore can act alone on feruloyled xylans Aspergillus nidulans ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 FAE
-
 Aspergillus nidulans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 37
-
 assay at Aspergillus nidulans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.73 9.5 11.8 feruloyl arabino-xylo-oligosaccharides recombinant His-tagged enzyme, pH 7.0, 37°C Aspergillus nidulans
3.1.1.73 10.1
-
 methyl ferulate recombinant His-tagged enzyme, pH 7.0, 37°C Aspergillus nidulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 7
-
 assay at Aspergillus nidulans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.73 10.5
-
 methyl ferulate recombinant His-tagged enzyme, pH 7.0, 37°C Aspergillus nidulans
3.1.1.73 29.7 51.3 feruloyl arabino-xylo-oligosaccharides recombinant His-tagged enzyme, pH 7.0, 37°C Aspergillus nidulans