Literature summary extracted from

Mukhopadhyay, A.; Dasgupta, A.K.; Chattopadhyay, D.; Chakrabarti, K.
Improvement of thermostability and activity of pectate lyase in the presence of hydroxyapatite nanoparticles (2012), Biores. Technol., 116, 348-354.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
4.2.2.2	additional information	activity of pectate lyase is 9fold higher at 90°C in the presence of hydroxyapatite nanoparticles than in the presence of 1 mM CaCl2	Priestia megaterium

Protein Variants

EC Number	Protein Variants	Comment	Organism
4.2.2.2	additional information	improvement of thermostability and activity of pectate lyase in the presence of hydroxyapatite nanoparticles	Priestia megaterium

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
4.2.2.2	additional information	-	additional information	enzyme kinetics and thermodynamics at 75°C and 90°C in presence or absence of hydroxyapatite nanoparticles, detailed overview	Priestia megaterium

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
4.2.2.2	Ca2+	activates, optimal at 1 mM	Priestia megaterium

Organism

EC Number	Organism	UniProt	Comment	Textmining
4.2.2.2	Priestia megaterium	-	-	-
4.2.2.2	Priestia megaterium AK2	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
4.2.2.2	native enzyme 18.6fold from strain AK2 culture supernatant by cation exchange chromatography and gel filtration	Priestia megaterium

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
4.2.2.2	852	-	purified native enzyme, pH 8.5, 55°C	Priestia megaterium

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
4.2.2.2	55	-	assay at	Priestia megaterium

Temperature Stability [°C]

EC Number	Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
4.2.2.2	additional information	-	improvement of thermostability and activity of pectate lyase in the presence of hydroxyapatite nanoparticles	Priestia megaterium
4.2.2.2	90	-	half-life of pectate lyase is 60fold higher in the presence of hydroxyapatite nanoparticles than in the presence of 1 mM CaCl2. Thermodynamic analysis of the nanoparticle-induced stability reveals an enhanced entropy-enthalpy compensation by hydroxyapatite nanoparticles since a reciprocal linearity of the enthalpy-entropy change to 90°C is observed	Priestia megaterium

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4.2.2.2	8.5	-	assay at	Priestia megaterium

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Release 2023.1 (January 2023)