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Literature summary extracted from
- Bacterial Anabaena variabilis phenylalanine ammonia lyase: A biocatalyst with broad substrate specificity (2014), Bioorg. Med. Chem., 22, 5555-5557.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.3.1.24 | expressed in Escherichia coli as a His-tagged fusion protein | Trichormus variabilis |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.3.1.24 | 0.03 | - |
(2E)-3-phenylprop-2-enoic acid | pH 8.3, 30°C | Trichormus variabilis |  |
| 4.3.1.24 | 0.07 | - |
(2E)-3-(3-fluorophenyl)prop-2-enoic acid | pH 8.3, 30°C | Trichormus variabilis | |
| 4.3.1.24 | 0.12 | - |
(2E)-3-(4-fluorophenyl)prop-2-enoic acid | pH 8.3, 30°C | Trichormus variabilis | |
| 4.3.1.24 | 0.23 | - |
(2S)-2-amino-3-(4-nitrophenyl)propanoic acid | pH 8.3, 30°C | Trichormus variabilis | |
| 4.3.1.24 | 0.29 | - |
(2S)-2-amino-3-phenylpropanoic acid | pH 8.3, 30°C | Trichormus variabilis | |
| 4.3.1.24 | 0.38 | - |
(2E)-3-(pyridin-4-yl)prop-2-enoic acid | pH 8.3, 30°C | Trichormus variabilis | |
| 4.3.1.24 | 0.39 | - |
(2E)-3-(pyridin-3-yl)prop-2-enoic acid | pH 8.3, 30°C | Trichormus variabilis | |
| 4.3.1.24 | 0.48 | - |
(2S)-2-amino-3-(pyridin-4-yl)propanoic acid | pH 8.3, 30°C | Trichormus variabilis | |
| 4.3.1.24 | 0.56 | - |
(2S)-2-amino-3-(4-fluorophenyl)propanoic acid | pH 8.3, 30°C | Trichormus variabilis | |
| 4.3.1.24 | 0.66 | - |
(2E)-3-(4-nitrophenyl)prop-2-enoic acid | pH 8.3, 30°C | Trichormus variabilis | |
| 4.3.1.24 | 0.75 | - |
(2S)-2-amino-3-(3-fluorophenyl)propanoic acid | pH 8.3, 30°C | Trichormus variabilis | |
| 4.3.1.24 | 1.05 | - |
(2S)-2-amino-3-[4-(trifluoromethyl)phenyl]propanoic acid | pH 8.3, 30°C | Trichormus variabilis | |
| 4.3.1.24 | 1.11 | - |
(2S)-2-amino-3-(3-hydroxyphenyl)propanoic acid | pH 8.3, 30°C | Trichormus variabilis | |
| 4.3.1.24 | 1.51 | - |
(2E)-3-[4-(trifluoromethyl)phenyl]prop-2-enoic acid | pH 8.3, 30°C | Trichormus variabilis | |
| 4.3.1.24 | 3.3 | - |
(2E)-3-(3-hydroxyphenyl)prop-2-enoic acid | pH 8.3, 30°C | Trichormus variabilis | |
| 4.3.1.24 | 3.39 | - |
(2S)-2-amino-3-(pyridin-3-yl)propanoic acid | pH 8.3, 30°C | Trichormus variabilis | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.3.1.24 | Trichormus variabilis | Q3M5Z3 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.3.1.24 | using Ni-NTA chromatography | Trichormus variabilis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.3.1.24 | (2S)-2-amino-3-(3-fluorophenyl)propanoic acid | - |
Trichormus variabilis | (2E)-3-(3-fluorophenyl)prop-2-enoic acid + NH3 | - |
r | |
| 4.3.1.24 | (2S)-2-amino-3-(3-hydroxyphenyl)propanoic acid | - |
Trichormus variabilis | (2E)-3-(3-hydroxyphenyl)prop-2-enoic acid + NH3 | - |
r | |
| 4.3.1.24 | (2S)-2-amino-3-(4-fluorophenyl)propanoic acid | - |
Trichormus variabilis | (2E)-3-(4-fluorophenyl)prop-2-enoic acid + NH3 | - |
r | |
| 4.3.1.24 | (2S)-2-amino-3-(4-nitrophenyl)propanoic acid | - |
Trichormus variabilis | (2E)-3-(4-nitrophenyl)prop-2-enoic acid + NH3 | - |
r | |
| 4.3.1.24 | (2S)-2-amino-3-(pyridin-3-yl)propanoic acid | - |
Trichormus variabilis | (2E)-3-(pyridin-3-yl)prop-2-enoic acid + NH3 | - |
r | |
| 4.3.1.24 | (2S)-2-amino-3-(pyridin-4-yl)propanoic acid | - |
Trichormus variabilis | (2E)-3-(pyridin-4-yl)prop-2-enoic acid + NH3 | - |
r | |
| 4.3.1.24 | (2S)-2-amino-3-phenylpropanoic acid | - |
Trichormus variabilis | (2E)-3-phenylprop-2-enoic acid + NH3 | - |
r | |
| 4.3.1.24 | (2S)-2-amino-3-[4-(trifluoromethyl)phenyl]propanoic acid | - |
Trichormus variabilis | (2E)-3-[4-(trifluoromethyl)phenyl]prop-2-enoic acid + NH3 | - |
r | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.3.1.24 | AvPAL | - |
Trichormus variabilis |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.3.1.24 | 0.03 | - |
(2E)-3-phenylprop-2-enoic acid | pH 8.3, 30°C | Trichormus variabilis | |
| 4.3.1.24 | 0.08 | - |
(2E)-3-(3-fluorophenyl)prop-2-enoic acid | pH 8.3, 30°C | Trichormus variabilis | |
| 4.3.1.24 | 0.09 | - |
(2E)-3-(4-fluorophenyl)prop-2-enoic acid | pH 8.3, 30°C | Trichormus variabilis | |
| 4.3.1.24 | 0.14 | - |
(2E)-3-(pyridin-3-yl)prop-2-enoic acid | pH 8.3, 30°C | Trichormus variabilis | |
| 4.3.1.24 | 0.49 | - |
(2S)-2-amino-3-phenylpropanoic acid | pH 8.3, 30°C | Trichormus variabilis | |
| 4.3.1.24 | 0.61 | - |
(2E)-3-(3-hydroxyphenyl)prop-2-enoic acid | pH 8.3, 30°C | Trichormus variabilis | |
| 4.3.1.24 | 0.61 | - |
(2S)-2-amino-3-(pyridin-4-yl)propanoic acid | pH 8.3, 30°C | Trichormus variabilis | |
| 4.3.1.24 | 0.82 | - |
(2S)-2-amino-3-[4-(trifluoromethyl)phenyl]propanoic acid | pH 8.3, 30°C | Trichormus variabilis | |
| 4.3.1.24 | 0.89 | - |
(2S)-2-amino-3-(3-hydroxyphenyl)propanoic acid | pH 8.3, 30°C | Trichormus variabilis | |
| 4.3.1.24 | 0.91 | - |
(2S)-2-amino-3-(pyridin-3-yl)propanoic acid | pH 8.3, 30°C | Trichormus variabilis | |
| 4.3.1.24 | 1.06 | - |
(2S)-2-amino-3-(4-fluorophenyl)propanoic acid | pH 8.3, 30°C | Trichormus variabilis | |
| 4.3.1.24 | 1.06 | - |
(2E)-3-(pyridin-4-yl)prop-2-enoic acid | pH 8.3, 30°C | Trichormus variabilis | |
| 4.3.1.24 | 1.08 | - |
(2S)-2-amino-3-(4-nitrophenyl)propanoic acid | pH 8.3, 30°C | Trichormus variabilis | |
| 4.3.1.24 | 1.1 | - |
(2S)-2-amino-3-(3-fluorophenyl)propanoic acid | pH 8.3, 30°C | Trichormus variabilis | |
| 4.3.1.24 | 3.14 | - |
(2E)-3-[4-(trifluoromethyl)phenyl]prop-2-enoic acid | pH 8.3, 30°C | Trichormus variabilis | |
| 4.3.1.24 | 3.81 | - |
(2E)-3-(4-nitrophenyl)prop-2-enoic acid | pH 8.3, 30°C | Trichormus variabilis | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.3.1.24 | 0.27 | - |
(2S)-2-amino-3-(pyridin-3-yl)propanoic acid | pH 8.3, 30°C | Trichormus variabilis | |
| 4.3.1.24 | 0.78 | - |
(2S)-2-amino-3-[4-(trifluoromethyl)phenyl]propanoic acid | pH 8.3, 30°C | Trichormus variabilis | |
| 4.3.1.24 | 0.8 | - |
(2S)-2-amino-3-(3-hydroxyphenyl)propanoic acid | pH 8.3, 30°C | Trichormus variabilis | |
| 4.3.1.24 | 1.27 | - |
(2S)-2-amino-3-(pyridin-4-yl)propanoic acid | pH 8.3, 30°C | Trichormus variabilis | |
| 4.3.1.24 | 1.47 | - |
(2S)-2-amino-3-(3-fluorophenyl)propanoic acid | pH 8.3, 30°C | Trichormus variabilis | |
| 4.3.1.24 | 1.69 | - |
(2S)-2-amino-3-phenylpropanoic acid | pH 8.3, 30°C | Trichormus variabilis | |
| 4.3.1.24 | 1.89 | - |
(2S)-2-amino-3-(4-fluorophenyl)propanoic acid | pH 8.3, 30°C | Trichormus variabilis | |
| 4.3.1.24 | 4.7 | - |
(2S)-2-amino-3-(4-nitrophenyl)propanoic acid | pH 8.3, 30°C | Trichormus variabilis | |
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