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Literature summary extracted from

  • Gayen, S.; Ghosh, U.
    Purification and characterization of tannin acyl hydrolase produced by mixed solid state fermentation of wheat bran and marigold flower by Penicillium notatum NCIM 923 (2013), BioMed Res. Int., 2013, 596380.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.20 3.3
-
 Tannic acid purified enzyme, at pH 5.0 and 37°C Penicillium chrysogenum
3.1.1.20 6.6
-
 Tannic acid crude enzyme, at pH 5.0 and 37°C Penicillium chrysogenum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.20 43000
-
 1 * 97000 + 1 * 43000, SDS-PAGE Penicillium chrysogenum
3.1.1.20 97000
-
 1 * 97000 + 1 * 43000, SDS-PAGE Penicillium chrysogenum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.20 digallate + H2O Penicillium chrysogenum
-
 gallate
-
 ?
3.1.1.20 digallate + H2O Penicillium chrysogenum NCIM 923
-
 gallate
-
 ?
3.1.1.20 tannic acid + H2O Penicillium chrysogenum
-
 gallate + D-glucose
-
 ?
3.1.1.20 tannic acid + H2O Penicillium chrysogenum NCIM 923
-
 gallate + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.20 Penicillium chrysogenum
-
-
-
3.1.1.20 Penicillium chrysogenum NCIM 923
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.20 ammonium sulfate precipitation and DEAE cellulose column chromatography Penicillium chrysogenum

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.20 digallate + H2O
-
 Penicillium chrysogenum gallate
-
 ?
3.1.1.20 digallate + H2O
-
 Penicillium chrysogenum NCIM 923 gallate
-
 ?
3.1.1.20 epigallocatechin gallate + H2O
-
 Penicillium chrysogenum gallate + epigallocatechin
-
 ?
3.1.1.20 epigallocatechin gallate + H2O
-
 Penicillium chrysogenum NCIM 923 gallate + epigallocatechin
-
 ?
3.1.1.20 gallotannin + H2O
-
 Penicillium chrysogenum gallate + D-glucose
-
 ?
3.1.1.20 gallotannin + H2O
-
 Penicillium chrysogenum NCIM 923 gallate + D-glucose
-
 ?
3.1.1.20 methyl gallate + H2O
-
 Penicillium chrysogenum gallate + methanol
-
 ?
3.1.1.20 methyl gallate + H2O
-
 Penicillium chrysogenum NCIM 923 gallate + methanol
-
 ?
3.1.1.20 tannic acid + H2O
-
 Penicillium chrysogenum gallate + D-glucose
-
 ?
3.1.1.20 tannic acid + H2O
-
 Penicillium chrysogenum NCIM 923 gallate + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.20 heterodimer 1 * 97000 + 1 * 43000, SDS-PAGE Penicillium chrysogenum

Synonyms

EC Number Synonyms Comment Organism
3.1.1.20 tannin acyl hydrolase
-
 Penicillium chrysogenum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.20 35 40
-
 Penicillium chrysogenum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.20 40 60 after 12 h incubation, above 95% activity is retained at 40°C, while the enzyme retains more than 60% of its activity at 60°C Penicillium chrysogenum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.20 5
-
-
 Penicillium chrysogenum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.20 3 8 after 12 h incubation, the enzyme retains 40% activity at pH 3.0 and 8.0, respectively Penicillium chrysogenum