Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bordon, K.C.; Perino, M.G.; Giglio, J.R.; Arantes, E.C.
    Isolation, enzymatic characterization and antiedematogenic activity of the first reported rattlesnake hyaluronidase from Crotalus durissus terrificus venom (2012), Biochimie, 94, 2740-2748.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.35 Ca2+ complete inhibition at 0.2 mM Crotalus durissus terrificus
3.2.1.35 Mg2+ complete inhibition at 0.2 mM Crotalus durissus terrificus
3.2.1.35 NaCl 1 M NaCl significantly reduces the enzyme activity Crotalus durissus terrificus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.35 NaCl maximum activity in the presence of 0.2 M NaCl Crotalus durissus terrificus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.35 64500
-
 1 * 64500, SDS-PAGE Crotalus durissus terrificus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.35 hyaluronan + 2 H2O Crotalus durissus terrificus highly specific substrate with 10fold higher activity compared to chondroitin 4-sulfate, chondroitin 6-sulfate, or dermatan N-acetyl-beta-D-glucosamine + D-glucuronate + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.35 Crotalus durissus terrificus B3EWP2
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.35 glycoprotein
-
 Crotalus durissus terrificus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.35 CM-cellulose-52 column chromatography, phenyl-Sepharose column chromatography, and Sephacryl S-100 gel filtration Crotalus durissus terrificus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.35 venom
-
 Crotalus durissus terrificus
-

Storage Stability

EC Number Storage Stability Organism
3.2.1.35 7°C, purification buffer, 60 days, remains stable Crotalus durissus terrificus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.35 chondroitin 4-sulfate + H2O
-
 Crotalus durissus terrificus ?
-
 ?
3.2.1.35 chondroitin 6-sulfate + H2O
-
 Crotalus durissus terrificus ?
-
 ?
3.2.1.35 dermatan + H2O
-
 Crotalus durissus terrificus ?
-
 ?
3.2.1.35 hyaluronan + 2 H2O highly specific substrate with 10fold higher activity compared to chondroitin 4-sulfate, chondroitin 6-sulfate, or dermatan Crotalus durissus terrificus N-acetyl-beta-D-glucosamine + D-glucuronate + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.35 monomer 1 * 64500, SDS-PAGE Crotalus durissus terrificus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.35 Hya1
-
 Crotalus durissus terrificus
3.2.1.35 hyaluronidase
-
 Crotalus durissus terrificus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.35 37
-
-
 Crotalus durissus terrificus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.35 25 50 more than 50% activity between 25 and 50°C Crotalus durissus terrificus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.35 5.5
-
-
 Crotalus durissus terrificus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.35 4 7 about 40% activity at pH 4.0, more than 90% activity at pH 5.0-6.0, about 50% activity at pH 6.5, about 10% activity at pH 7.0 Crotalus durissus terrificus