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Literature summary extracted from

  • Revtovich, S.V.; Faleev, N.G.; Morozova, E.A.; Anufrieva, N.V.; Nikulin, A.D.; Demidkina, T.V.
    Crystal structure of the external aldimine of Citrobacter freundii methionine gamma-lyase with glycine provides insight in mechanisms of two stages of physiological reaction and isotope exchange of alpha- and beta-protons of competitive inhibitors (2014), Biochimie, 101, 161-167.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.4.1.11 purified recombinant enzyme in complex with inhibitor glycine, crystals are obtained using a method without the presence of ammonium sulfate, complexing with glycine by soaking of holoenzyme crystals in a cryoprotective mother liquid solution to which glycine is added stepwise from 5 mM to 20 mM during 20 min,, 1-2 weeks, X-ray diffraction structure determination and analysis at 2.45 A resolution, molecular replacement Citrobacter freundii

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.11 glycine competitive inhibitor, subtle conformational changes provide effective binding of the inhibitor and facilitate labilization of Calpha-protons of the external aldimine, binding structure, overview Citrobacter freundii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.11 L-methionine + H2O Citrobacter freundii gamma-elimination reaction methanethiol + NH3 + 2-oxobutanoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.11 Citrobacter freundii Q84AR1
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.4.1.11 L-methionine + H2O = methanethiol + NH3 + 2-oxobutanoate gamma-elimination reaction mechanism and intermediates, overview Citrobacter freundii
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.11 L-methionine + H2O gamma-elimination reaction Citrobacter freundii methanethiol + NH3 + 2-oxobutanoate
-
 ?
4.4.1.11 L-methionine + H2O gamma-elimination reaction, initial aldimine, quinonoid, and ketimine intermediates of the gamma-elimination reaction, possible concerted mechanisms of reversible ketimine formation and pro-(R)-Calpha-proton exchange, overview Citrobacter freundii methanethiol + NH3 + 2-oxobutanoate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.4.1.11 MGL
-
 Citrobacter freundii

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.11 pyridoxal 5'-phosphate
-
 Citrobacter freundii

General Information

EC Number General Information Comment Organism
4.4.1.11 additional information catalytic residues: Lys210 is the base, and Tyr113 acts as a general acid, active site structure, overview Citrobacter freundii