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Literature summary extracted from

  • Kolomytseva, M.; Ferraroni, M.; Chernykh, A.; Golovleva, L.; Scozzafava, A.
    Structural basis for the substrate specificity and the absence of dehalogenation activity in 2-chloromuconate cycloisomerase from Rhodococcus opacus 1CP (2014), Biochim. Biophys. Acta, 1844, 1541-1549.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.5.1.1 the crystallographic structure of the chloromuconate cycloisomerase from Rhodococcus opacus is determined at 2.5 A of resolution. Results highlight that a histidine, located in a loop that closes the active site cavity upon the binding of the substrate, could be related to the dehalogenation inability of Rho-2-CMCI Rhodococcus opacus
5.5.1.7 the crystallographic structure of the homooctameric Rho-2-CMCI is solved at 2.5 A resolution by molecular replacement using the coordinates of the structure of chloromuconate cycloisomerase from Pseudomonas putida PRS2000 Rhodococcus opacus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.5.1.7 0.0487
-
 3-methyl-cis,cis-muconate pH and temperature not specified in the publication Rhodococcus opacus
5.5.1.7 0.0559
-
 2-chloro-cis,cis-muconate pH and temperature not specified in the publication Rhodococcus opacus
5.5.1.7 0.0813
-
 cis,cis-muconate pH and temperature not specified in the publication Rhodococcus opacus
5.5.1.7 1.24
-
 3-chloro-cis,cis-muconate pH and temperature not specified in the publication Rhodococcus opacus

Organism

EC Number Organism UniProt Comment Textmining
5.5.1.1 Rhodococcus opacus
-
-
-
5.5.1.7 Rhodococcus opacus Q8G9L1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.5.1.7 anion-exchange chromatography on DEAE-Toyopearl, hydrophobic chromatography on Phenyl-sepharose with linear gradient (NH4)2SO4, anion-exchange Q3 chromatography and hydrophobic chromatography Rhodococcus opacus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.5.1.7 2-Chloro-cis,cis-muconate
-
 Rhodococcus opacus 4-Carboxymethyl-2-chloro-but-2-en-4-olide
-
 ?
5.5.1.7 3-Chloro-cis,cis-muconate
-
 Rhodococcus opacus cis-4-Carboxymethylenebut-2-en-4-olide
-
 ?
5.5.1.7 3-Methyl-cis,cis-muconate
-
 Rhodococcus opacus ?
-
 ?
5.5.1.7 cis,cis-Muconate
-
 Rhodococcus opacus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.5.1.1 homooctamer
-
 Rhodococcus opacus
5.5.1.7 homooctamer
-
 Rhodococcus opacus

Synonyms

EC Number Synonyms Comment Organism
5.5.1.1 2-chloromuconate cycloisomerase
-
 Rhodococcus opacus
5.5.1.1 Rho-2-CMCI
-
 Rhodococcus opacus
5.5.1.7 2-chloromuconate cycloisomerase
-
 Rhodococcus opacus
5.5.1.7 Rho-2-CMCl
-
 Rhodococcus opacus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.5.1.7 3.2
-
 3-methyl-cis,cis-muconate pH and temperature not specified in the publication Rhodococcus opacus
5.5.1.7 3.6
-
 2-chloro-cis,cis-muconate pH and temperature not specified in the publication Rhodococcus opacus
5.5.1.7 8.8
-
 3-chloro-cis,cis-muconate pH and temperature not specified in the publication Rhodococcus opacus
5.5.1.7 24.66
-
 cis,cis-muconate pH and temperature not specified in the publication Rhodococcus opacus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.5.1.7 6.66
-
 3-chloro-cis,cis-muconate pH and temperature not specified in the publication Rhodococcus opacus
5.5.1.7 65
-
 2-chloro-cis,cis-muconate pH and temperature not specified in the publication Rhodococcus opacus
5.5.1.7 65
-
 3-methyl-cis,cis-muconate pH and temperature not specified in the publication Rhodococcus opacus
5.5.1.7 303
-
 cis,cis-muconate pH and temperature not specified in the publication Rhodococcus opacus