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Literature summary extracted from

  • Gorina, S.S.; Toporkova, Y.Y.; Mukhtarova, L.S.; Chechetkin, I.R.; Khairutdinov, B.I.; Gogolev, Y.V.; Grechkin, A.N.
    Detection and molecular cloning of CYP74Q1 gene: identification of Ranunculus acris leaf divinyl ether synthase (2014), Biochim. Biophys. Acta, 1841, 1227-1233.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.B8 gene CYP74Q1, DNA and amino acid sequence determination and analysis, sequence comparisons Ranunculus acris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.B8 0.0431
-
 (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienate pH 7.5, 25°C Ranunculus acris
4.2.1.B8 0.0976
-
 (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid pH 7.5, 25°C Ranunculus acris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.B8 (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid Ranunculus acris
-
 (9Z,11E)-12-[(1'Z)-hexenyloxy]-9,11-dodecadienoic acid i.e. (omega5Z)-etherolenic acid ?
4.2.1.B8 (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoate Ranunculus acris highly preferred substrate (9Z,11E)-12-[(1'Z,3'Z)-hexadienyloxy]-9,11-dodecadienoate + H2O i.e. etherolenic acid ?
4.2.1.B8 additional information Ranunculus acris preferred substrates of the enzyme are the 13-hydroperoxides of alpha-linolenic and linoleic acids, which are converted to the divinyl ether oxylipins (omega5Z)-etherolenic acid, (9Z,11E)-12-[(1?Z,3?Z)-hexadienyloxy]-9,11-dodecadienoic acid, and (omega5Z)-etheroleic acid, (9Z,11E)-12-[(1?Z)-hexenyloxy]-9,11-dodecadienoic acid, respectively, as revealed by the data of mass spectrometry, NMR and UV spectroscopy ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.B8 Ranunculus acris
-
 two CYP74Q1 isoforms, gene CYP74Q1
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.B8 leaf
-
 Ranunculus acris
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.B8 (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid
-
 Ranunculus acris (9Z,11E)-12-[(1'Z)-hexenyloxy]-9,11-dodecadienoic acid i.e. (omega5Z)-etherolenic acid ?
4.2.1.B8 (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoate highly preferred substrate Ranunculus acris (9Z,11E)-12-[(1'Z,3'Z)-hexadienyloxy]-9,11-dodecadienoate + H2O i.e. etherolenic acid ?
4.2.1.B8 additional information preferred substrates of the enzyme are the 13-hydroperoxides of alpha-linolenic and linoleic acids, which are converted to the divinyl ether oxylipins (omega5Z)-etherolenic acid, (9Z,11E)-12-[(1?Z,3?Z)-hexadienyloxy]-9,11-dodecadienoic acid, and (omega5Z)-etheroleic acid, (9Z,11E)-12-[(1?Z)-hexenyloxy]-9,11-dodecadienoic acid, respectively, as revealed by the data of mass spectrometry, NMR and UV spectroscopy Ranunculus acris ?
-
 ?
4.2.1.B8 additional information no activity with (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid and (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid, high substrate specificity, overview. Product identification by GC-MS analyses Ranunculus acris ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.B8 CYP74Q1
-
 Ranunculus acris
4.2.1.B8 DES
-
 Ranunculus acris
4.2.1.B8 divinyl ether synthase
-
 Ranunculus acris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.B8 25
-
 assay at Ranunculus acris

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.B8 67.7
-
 (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid pH 7.5, 25°C Ranunculus acris
4.2.1.B8 134
-
 (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienate pH 7.5, 25°C Ranunculus acris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.B8 7.5
-
 assay at Ranunculus acris

General Information

EC Number General Information Comment Organism
4.2.1.B8 evolution the enzyme belongs to the CYP74 family, phylogenetic tree Ranunculus acris
4.2.1.B8 physiological function enzymes of the CYP74 family, including the divinyl ether synthase, play important roles in plant cell signalling and defence Ranunculus acris

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.1.B8 0.0007
-
 (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid pH 7.5, 25°C Ranunculus acris
4.2.1.B8 0.0031
-
 (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienate pH 7.5, 25°C Ranunculus acris