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Literature summary extracted from

  • Park, K.H.; Kim, M.G.; Ahn, H.J.; Lee, D.H.; Kim, J.H.; Kim, Y.W.; Woo, E.J.
    Structural and biochemical characterization of the broad substrate specificity of Bacteroides thetaiotaomicron commensal sialidase (2013), Biochim. Biophys. Acta, 1834, 1510-1519.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.18 expressed in Escherichia coli BL21(DE3) cells Bacteroides thetaiotaomicron

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.18 sitting drop vapor diffusion method, using 20% (w/v) polyethylene glycol 4000, 0.2 M ammonium citrate tri-basic (pH 7.0) and 0.1 M Tris pH 8.5 Bacteroides thetaiotaomicron

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.18 2-deoxy-2,3-dehydro-N-acetyl neuraminic acid competitive inhibition Bacteroides thetaiotaomicron
3.2.1.18 Cu2+ 2.9% residual activity at 5 mM Bacteroides thetaiotaomicron
3.2.1.18 Fe2+ 33.7% residual activity at 5 mM Bacteroides thetaiotaomicron
3.2.1.18 additional information EDTA does not cause a significant decrease in activity Bacteroides thetaiotaomicron
3.2.1.18 SDS 0.9% residual activity at 5 mM Bacteroides thetaiotaomicron
3.2.1.18 Zn2+ 31.9% residual activity at 5 mM Bacteroides thetaiotaomicron

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.18 0.11
-
 4-nitrophenyl alpha-D-N-acetylneuraminic acid pH 7.0 and 37°C Bacteroides thetaiotaomicron
3.2.1.18 0.16
-
 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid pH 7.0 and 37°C Bacteroides thetaiotaomicron
3.2.1.18 0.29
-
 colominic acid pH 7.0 and 37°C Bacteroides thetaiotaomicron
3.2.1.18 0.49
-
 sialyl-alpha-2,6-lactose pH 7.0 and 37°C Bacteroides thetaiotaomicron
3.2.1.18 1.47
-
 sialyl-alpha-2,3-lactose pH 7.0 and 37°C Bacteroides thetaiotaomicron

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.18 Ca2+ the activity is maintained more than 94% in the presence of Ca2+ at concentrations up to 5 mM Bacteroides thetaiotaomicron
3.2.1.18 Mg2+ the activity is maintained more than 96% in the presence of Mg2+ at concentrations up to 5 mM Bacteroides thetaiotaomicron
3.2.1.18 Mn2+ the activity is maintained more than 69% in the presence of Mn2+ at concentrations up to 5 mM Bacteroides thetaiotaomicron
3.2.1.18 Ni2+ the activity is maintained more than 75% in the presence of Ni2+ at concentrations up to 5 mM Bacteroides thetaiotaomicron

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.18 Bacteroides thetaiotaomicron Q8AAK9
-
-
3.2.1.18 Bacteroides thetaiotaomicron VPI-5482 Q8AAK9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.18 Ni-affinity column chromatography Bacteroides thetaiotaomicron

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.18 8.6
-
 with transferrin as substrate, pH 7.0 and 37°C Bacteroides thetaiotaomicron
3.2.1.18 18.2
-
 with alpha1-acid glycoprotein as substrate, pH 7.0 and 37°C Bacteroides thetaiotaomicron
3.2.1.18 35
-
 with fetuin as substrate, pH 7.0 and 37°C Bacteroides thetaiotaomicron
3.2.1.18 64.1
-
 with colominic acid as substrate, pH 7.0 and 37°C Bacteroides thetaiotaomicron
3.2.1.18 97.7
-
 with sialyl-alpha-2,3-lactose as substrate, pH 7.0 and 37°C Bacteroides thetaiotaomicron
3.2.1.18 100.9
-
 with sialyl-alpha-2,6-lactose as substrate, pH 7.0 and 37°C Bacteroides thetaiotaomicron

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.18 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid + H2O
-
 Bacteroides thetaiotaomicron 4-methylumbelliferone + N-acetylneuraminic acid
-
 ?
3.2.1.18 4-nitrophenyl alpha-D-N-acetylneuraminic acid + H2O
-
 Bacteroides thetaiotaomicron 4-nitrophenol + N-acetylneuraminic acid
-
 ?
3.2.1.18 alpha1-acid glycoprotein + H2O
-
 Bacteroides thetaiotaomicron ?
-
 ?
3.2.1.18 alpha1-acid glycoprotein + H2O
-
 Bacteroides thetaiotaomicron VPI-5482 ?
-
 ?
3.2.1.18 colominic acid + H2O
-
 Bacteroides thetaiotaomicron ?
-
 ?
3.2.1.18 colominic acid + H2O
-
 Bacteroides thetaiotaomicron VPI-5482 ?
-
 ?
3.2.1.18 fetuin + H2O
-
 Bacteroides thetaiotaomicron ?
-
 ?
3.2.1.18 fetuin + H2O
-
 Bacteroides thetaiotaomicron VPI-5482 ?
-
 ?
3.2.1.18 sialyl-alpha-2,3-lactose + H2O
-
 Bacteroides thetaiotaomicron ?
-
 ?
3.2.1.18 sialyl-alpha-2,3-lactose + H2O
-
 Bacteroides thetaiotaomicron VPI-5482 ?
-
 ?
3.2.1.18 sialyl-alpha-2,6-lactose + H2O
-
 Bacteroides thetaiotaomicron ?
-
 ?
3.2.1.18 sialyl-alpha-2,6-lactose + H2O
-
 Bacteroides thetaiotaomicron VPI-5482 ?
-
 ?
3.2.1.18 transferrin + H2O
-
 Bacteroides thetaiotaomicron ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.18 neuraminidase
-
 Bacteroides thetaiotaomicron
3.2.1.18 sialidase
-
 Bacteroides thetaiotaomicron

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.18 40
-
-
 Bacteroides thetaiotaomicron

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.18 30 50 more than 60% of activity is maintained from 30 to 50°C Bacteroides thetaiotaomicron

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.18 82.4
-
 4-nitrophenyl alpha-D-N-acetylneuraminic acid pH 7.0 and 37°C Bacteroides thetaiotaomicron
3.2.1.18 146.7
-
 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid pH 7.0 and 37°C Bacteroides thetaiotaomicron
3.2.1.18 157.6
-
 colominic acid pH 7.0 and 37°C Bacteroides thetaiotaomicron
3.2.1.18 161
-
 sialyl-alpha-2,6-lactose pH 7.0 and 37°C Bacteroides thetaiotaomicron
3.2.1.18 313.5
-
 sialyl-alpha-2,3-lactose pH 7.0 and 37°C Bacteroides thetaiotaomicron

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.18 7
-
 in 50 mM Tris-HCl buffer Bacteroides thetaiotaomicron

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.18 6 8 more than 70% of activity is maintained from pH 6.0 to 8.0 Bacteroides thetaiotaomicron

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.18 0.035
-
 2-deoxy-2,3-dehydro-N-acetyl neuraminic acid pH 7.0 and 37°C Bacteroides thetaiotaomicron

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.18 213.3
-
 sialyl-alpha-2,3-lactose pH 7.0 and 37°C Bacteroides thetaiotaomicron
3.2.1.18 328.6
-
 sialyl-alpha-2,6-lactose pH 7.0 and 37°C Bacteroides thetaiotaomicron
3.2.1.18 541.2
-
 colominic acid pH 7.0 and 37°C Bacteroides thetaiotaomicron
3.2.1.18 729.2
-
 4-nitrophenyl alpha-D-N-acetylneuraminic acid pH 7.0 and 37°C Bacteroides thetaiotaomicron
3.2.1.18 916.9
-
 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid pH 7.0 and 37°C Bacteroides thetaiotaomicron