Literature summary extracted from
Mizutani, K.; Toyoda, M.; Otake, Y.; Yoshioka, S.; Takahashi, N.; Mikami, B.
Structural and functional characterization of recombinant medaka fish alpha-amylase expressed in yeast Pichia pastoris (2012), Biochim. Biophys. Acta, 1824, 954-962.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.2.1.1 |
expression in Pichia pastoris |
Oryzias latipes |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
3.2.1.1 |
His-tagged recombinant protein, to 2.85 A resolution, space group P3221, one protein molecule per asymmetric unit. One bound calcium ion is found, coordinated to the side chains of Asn100 and Asp167, the main-chains of Arg158 and His201, and three water molecules. The overall structure is the TIM barrel structure with two additional domains, domain B protruding out of the barrel in the place of the beta3-alpha3 loop and domain C succeeding the catalytic barrel |
Oryzias latipes |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.2.1.1 |
additional information |
not inhibitory: NaCl up to 250 mM |
Oryzias latipes |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.2.1.1 |
Ca2+ |
in the crystal structure, one bound calcium ion is found, coordinated to the side chains of Asn100 and Asp167, the main-chains of Arg158 and His201, and three water molecules |
Oryzias latipes |
|
3.2.1.1 |
chloride |
presence of chloride ions is essential for activity. 20 mM NaCl is sufficient for maximum activity. NaCl is not inhibitory below 250 mM |
Oryzias latipes |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.2.1.1 |
Oryzias latipes |
H2N0D4 |
- |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.2.1.1 |
starch + H2O |
corn starch is preferred over wheat starch and sweet-potato starch. Potato starch is a poor substrate |
Oryzias latipes |
? |
- |
? |
|
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
3.2.1.1 |
50 |
- |
- |
Oryzias latipes |
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
3.2.1.1 |
55 |
- |
melting temperature |
Oryzias latipes |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.2.1.1 |
7 |
- |
- |
Oryzias latipes |