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Literature summary extracted from

  • Tian, Y.; Cui, W.; Huang, M.; Robinson, H.; Wan, Y.; Wang, Y.; Ke, H.
    Dual specificity and novel structural folding of yeast phosphodiesterase-1 for hydrolysis of second messengers cyclic adenosine and guanosine 3,5-monophosphate (2014), Biochemistry, 53, 4938-4945.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.17 expression in Escherichia coli Saccharomyces cerevisiae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.4.17 unliganded enzyme, and in complex with cGMP, both to 1.3 A resolution. For the massive interactions between two monomers, the dimeric form of the enzyme is a biologic unit for its function Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.17 0.0773
-
3',5'-cAMP pH 7.5, 22°C, presence of 1 mM EDTA Saccharomyces cerevisiae
3.1.4.17 0.0968
-
3',5'-cGMP pH 7.5, 22°C, presence of 10 mM MgCl2 Saccharomyces cerevisiae
3.1.4.17 0.0988
-
3',5'-cAMP pH 7.5, 22°C, presence of 10 mM MgCl2 Saccharomyces cerevisiae
3.1.4.17 0.1047
-
3',5'-cGMP pH 7.5, 22°C Saccharomyces cerevisiae
3.1.4.17 0.1048
-
3',5'-cGMP pH 7.5, 22°C, presence of 10 mM ZnCl2 Saccharomyces cerevisiae
3.1.4.17 0.11
-
3',5'-cAMP pH 7.5, 22°C Saccharomyces cerevisiae
3.1.4.17 0.1157
-
3',5'-cAMP pH 7.5, 22°C, presence of 10 mM ZnCl2 Saccharomyces cerevisiae
3.1.4.17 0.1175
-
3',5'-cAMP pH 7.5, 22°C, presence of 10 mM MnCl2 Saccharomyces cerevisiae
3.1.4.17 0.1539
-
3',5'-cGMP pH 7.5, 22°C, presence of 1 mM EDTA Saccharomyces cerevisiae
3.1.4.17 0.1913
-
3',5'-cGMP pH 7.5, 22°C, presence of 10 mM MnCl2 Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.17 Saccharomyces cerevisiae P22434
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.17 3',5'-cAMP + H2O
-
Saccharomyces cerevisiae 5'-AMP
-
?
3.1.4.17 3',5'-cGMP + H2O
-
Saccharomyces cerevisiae 5'-GMP
-
?
3.1.4.17 additional information enzyxme displays dual specificity for hydrolysis of both cAMP and cGMP Saccharomyces cerevisiae ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.17 PDE1
-
Saccharomyces cerevisiae
3.1.4.17 phosphodiesterase-1
-
Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.4.17 10.8
-
3',5'-cGMP pH 7.5, 22°C, presence of 10 mM ZnCl2 Saccharomyces cerevisiae
3.1.4.17 11.1
-
3',5'-cAMP pH 7.5, 22°C, presence of 1 mM EDTA Saccharomyces cerevisiae
3.1.4.17 11.7
-
3',5'-cGMP pH 7.5, 22°C, presence of 1 mM EDTA Saccharomyces cerevisiae
3.1.4.17 11.8
-
3',5'-cGMP pH 7.5, 22°C Saccharomyces cerevisiae
3.1.4.17 11.8
-
3',5'-cAMP pH 7.5, 22°C, presence of 10 mM MnCl2 Saccharomyces cerevisiae
3.1.4.17 14.3
-
3',5'-cGMP pH 7.5, 22°C, presence of 10 mM MnCl2 Saccharomyces cerevisiae
3.1.4.17 15.1
-
3',5'-cGMP pH 7.5, 22°C, presence of 10 mM MgCl2 Saccharomyces cerevisiae
3.1.4.17 16.9
-
3',5'-cAMP pH 7.5, 22°C Saccharomyces cerevisiae
3.1.4.17 17.4
-
3',5'-cAMP pH 7.5, 22°C, presence of 10 mM ZnCl2 Saccharomyces cerevisiae
3.1.4.17 21
-
3',5'-cAMP pH 7.5, 22°C, presence of 10 mM MgCl2 Saccharomyces cerevisiae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.4.17 75
-
3',5'-cGMP pH 7.5, 22°C, presence of 10 mM MnCl2 Saccharomyces cerevisiae
3.1.4.17 76
-
3',5'-cGMP pH 7.5, 22°C, presence of 1 mM EDTA Saccharomyces cerevisiae
3.1.4.17 100
-
3',5'-cAMP pH 7.5, 22°C, presence of 10 mM MnCl2 Saccharomyces cerevisiae
3.1.4.17 103
-
3',5'-cGMP pH 7.5, 22°C, presence of 10 mM ZnCl2 Saccharomyces cerevisiae
3.1.4.17 113
-
3',5'-cGMP pH 7.5, 22°C Saccharomyces cerevisiae
3.1.4.17 144
-
3',5'-cAMP pH 7.5, 22°C, presence of 1 mM EDTA Saccharomyces cerevisiae
3.1.4.17 150
-
3',5'-cAMP pH 7.5, 22°C, presence of 10 mM ZnCl2 Saccharomyces cerevisiae
3.1.4.17 153
-
3',5'-cAMP pH 7.5, 22°C Saccharomyces cerevisiae
3.1.4.17 156
-
3',5'-cGMP pH 7.5, 22°C, presence of 10 mM MgCl2 Saccharomyces cerevisiae
3.1.4.17 213
-
3',5'-cAMP pH 7.5, 22°C, presence of 10 mM MgCl2 Saccharomyces cerevisiae