EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.4.17 | expression in Escherichia coli | Saccharomyces cerevisiae |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.1.4.17 | unliganded enzyme, and in complex with cGMP, both to 1.3 A resolution. For the massive interactions between two monomers, the dimeric form of the enzyme is a biologic unit for its function | Saccharomyces cerevisiae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.4.17 | 0.0773 | - |
3',5'-cAMP | pH 7.5, 22°C, presence of 1 mM EDTA | Saccharomyces cerevisiae | |
3.1.4.17 | 0.0968 | - |
3',5'-cGMP | pH 7.5, 22°C, presence of 10 mM MgCl2 | Saccharomyces cerevisiae | |
3.1.4.17 | 0.0988 | - |
3',5'-cAMP | pH 7.5, 22°C, presence of 10 mM MgCl2 | Saccharomyces cerevisiae | |
3.1.4.17 | 0.1047 | - |
3',5'-cGMP | pH 7.5, 22°C | Saccharomyces cerevisiae | |
3.1.4.17 | 0.1048 | - |
3',5'-cGMP | pH 7.5, 22°C, presence of 10 mM ZnCl2 | Saccharomyces cerevisiae | |
3.1.4.17 | 0.11 | - |
3',5'-cAMP | pH 7.5, 22°C | Saccharomyces cerevisiae | |
3.1.4.17 | 0.1157 | - |
3',5'-cAMP | pH 7.5, 22°C, presence of 10 mM ZnCl2 | Saccharomyces cerevisiae | |
3.1.4.17 | 0.1175 | - |
3',5'-cAMP | pH 7.5, 22°C, presence of 10 mM MnCl2 | Saccharomyces cerevisiae | |
3.1.4.17 | 0.1539 | - |
3',5'-cGMP | pH 7.5, 22°C, presence of 1 mM EDTA | Saccharomyces cerevisiae | |
3.1.4.17 | 0.1913 | - |
3',5'-cGMP | pH 7.5, 22°C, presence of 10 mM MnCl2 | Saccharomyces cerevisiae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.4.17 | Saccharomyces cerevisiae | P22434 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.17 | 3',5'-cAMP + H2O | - |
Saccharomyces cerevisiae | 5'-AMP | - |
? | |
3.1.4.17 | 3',5'-cGMP + H2O | - |
Saccharomyces cerevisiae | 5'-GMP | - |
? | |
3.1.4.17 | additional information | enzyxme displays dual specificity for hydrolysis of both cAMP and cGMP | Saccharomyces cerevisiae | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.4.17 | PDE1 | - |
Saccharomyces cerevisiae |
3.1.4.17 | phosphodiesterase-1 | - |
Saccharomyces cerevisiae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.4.17 | 10.8 | - |
3',5'-cGMP | pH 7.5, 22°C, presence of 10 mM ZnCl2 | Saccharomyces cerevisiae | |
3.1.4.17 | 11.1 | - |
3',5'-cAMP | pH 7.5, 22°C, presence of 1 mM EDTA | Saccharomyces cerevisiae | |
3.1.4.17 | 11.7 | - |
3',5'-cGMP | pH 7.5, 22°C, presence of 1 mM EDTA | Saccharomyces cerevisiae | |
3.1.4.17 | 11.8 | - |
3',5'-cGMP | pH 7.5, 22°C | Saccharomyces cerevisiae | |
3.1.4.17 | 11.8 | - |
3',5'-cAMP | pH 7.5, 22°C, presence of 10 mM MnCl2 | Saccharomyces cerevisiae | |
3.1.4.17 | 14.3 | - |
3',5'-cGMP | pH 7.5, 22°C, presence of 10 mM MnCl2 | Saccharomyces cerevisiae | |
3.1.4.17 | 15.1 | - |
3',5'-cGMP | pH 7.5, 22°C, presence of 10 mM MgCl2 | Saccharomyces cerevisiae | |
3.1.4.17 | 16.9 | - |
3',5'-cAMP | pH 7.5, 22°C | Saccharomyces cerevisiae | |
3.1.4.17 | 17.4 | - |
3',5'-cAMP | pH 7.5, 22°C, presence of 10 mM ZnCl2 | Saccharomyces cerevisiae | |
3.1.4.17 | 21 | - |
3',5'-cAMP | pH 7.5, 22°C, presence of 10 mM MgCl2 | Saccharomyces cerevisiae |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.4.17 | 75 | - |
3',5'-cGMP | pH 7.5, 22°C, presence of 10 mM MnCl2 | Saccharomyces cerevisiae | |
3.1.4.17 | 76 | - |
3',5'-cGMP | pH 7.5, 22°C, presence of 1 mM EDTA | Saccharomyces cerevisiae | |
3.1.4.17 | 100 | - |
3',5'-cAMP | pH 7.5, 22°C, presence of 10 mM MnCl2 | Saccharomyces cerevisiae | |
3.1.4.17 | 103 | - |
3',5'-cGMP | pH 7.5, 22°C, presence of 10 mM ZnCl2 | Saccharomyces cerevisiae | |
3.1.4.17 | 113 | - |
3',5'-cGMP | pH 7.5, 22°C | Saccharomyces cerevisiae | |
3.1.4.17 | 144 | - |
3',5'-cAMP | pH 7.5, 22°C, presence of 1 mM EDTA | Saccharomyces cerevisiae | |
3.1.4.17 | 150 | - |
3',5'-cAMP | pH 7.5, 22°C, presence of 10 mM ZnCl2 | Saccharomyces cerevisiae | |
3.1.4.17 | 153 | - |
3',5'-cAMP | pH 7.5, 22°C | Saccharomyces cerevisiae | |
3.1.4.17 | 156 | - |
3',5'-cGMP | pH 7.5, 22°C, presence of 10 mM MgCl2 | Saccharomyces cerevisiae | |
3.1.4.17 | 213 | - |
3',5'-cAMP | pH 7.5, 22°C, presence of 10 mM MgCl2 | Saccharomyces cerevisiae |