Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Groninger-Poe, F.P.; Bouvier, J.T.; Vetting, M.W.; Kalyanaraman, C.; Kumar, R.; Almo, S.C.; Jacobson, M.P.; Gerlt, J.A.
    Evolution of enzymatic activities in the enolase superfamily: galactarate dehydratase III from Agrobacterium tumefaciens C58 (2014), Biochemistry, 53, 4192-4203.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.42 sitting drop vapor diffusion method, using 2 M ammonium sulfate and 100 mM Bis-Tris-HCl (pH 5.5) Agrobacterium tumefaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.42 0.08
-
 meso-galactarate at pH 7.9 and 22°C Agrobacterium tumefaciens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.42 Mg2+ required Agrobacterium tumefaciens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.42 meso-galactarate Agrobacterium tumefaciens
-
 2-dehydro-3-deoxy-D-glucarate + H2O
-
 ?
4.2.1.42 meso-galactarate Agrobacterium tumefaciens C58 / ATCC 33970
-
 2-dehydro-3-deoxy-D-glucarate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.42 Agrobacterium tumefaciens
-
-
-
4.2.1.42 Agrobacterium tumefaciens C58 / ATCC 33970
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.42 ammonium sulfate precipitation, column chromatography, and gel filtration Agrobacterium tumefaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.42 D-galacturonate low level activity Agrobacterium tumefaciens 3-deoxy-D-xylo-hexarate + 3-deoxy-D-lyxo-hexarate + H2O
-
 ?
4.2.1.42 D-galacturonate low level activity Agrobacterium tumefaciens C58 / ATCC 33970 3-deoxy-D-xylo-hexarate + 3-deoxy-D-lyxo-hexarate + H2O
-
 ?
4.2.1.42 meso-galactarate
-
 Agrobacterium tumefaciens 2-dehydro-3-deoxy-D-glucarate + H2O
-
 ?
4.2.1.42 meso-galactarate
-
 Agrobacterium tumefaciens C58 / ATCC 33970 2-dehydro-3-deoxy-D-glucarate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.42 A9CG74
-
 Agrobacterium tumefaciens
4.2.1.42 galactarate dehydratase III
-
 Agrobacterium tumefaciens
4.2.1.42 GalrD-III
-
 Agrobacterium tumefaciens
4.2.1.42 m-galactarate dehydratase
-
 Agrobacterium tumefaciens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.42 0.12
-
 meso-galactarate at pH 7.9 and 22°C Agrobacterium tumefaciens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.1.42 1.5
-
 meso-galactarate at pH 7.9 and 22°C Agrobacterium tumefaciens