EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.1.1 | expressed in Escherichia coli BL21(DE3) cells | Cereibacter sphaeroides |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.1.1.1 | sitting drop vapor diffusion method, using 25% (w/v) PEG 3350, 0.2 M MgCl2, and 0.1 M Tris, pH 8.5 | Cereibacter sphaeroides |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.1.1 | E175K | the mutant shows 10fold reduced Mn2+ content compared to the wild type enzyme | Cereibacter sphaeroides |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.1 | 0.024 | - |
4-methylumbelliferyl heptanoate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 0.033 | - |
4-nitrophenyl octanoate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 0.048 | - |
4-nitrophenyl hexanoate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 0.056 | - |
4-nitrophenyl butyrate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 0.11 | - |
paraoxon | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 0.16 | - |
4-Methylumbelliferyl butyrate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 0.18 | - |
1-naphthyl butyrate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 0.22 | - |
ethyl 4-nitrophenyl methylphosphonate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 0.24 | - |
4-methylumbelliferyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 0.33 | - |
ethyl butyrylacetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 0.4 | - |
2-naphthyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 0.42 | - |
methyl paraoxon | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 0.42 | - |
ethyl 4-nitrophenyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 0.52 | - |
4-nitrophenyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 0.56 | - |
2-naphthyl propionate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 0.62 | - |
ethyl 2-naphthyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 0.64 | - |
1-naphthyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 0.65 | - |
4-nitroacetanilide | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 0.7 | - |
isobutyl 4-nitrophenyl methylphosphonate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 0.9 | - |
1,4-diacetoxybenzene | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 1.2 | - |
4-methylbenzyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 1.2 | - |
isopropyl 4-nitrophenyl methylphosphonate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 1.6 | - |
Benzyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 1.6 | - |
4'-chloroacetanilide | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 1.8 | - |
3-acetoxypyridine | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 1.9 | - |
indoxyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 2.8 | - |
phenyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.1 | Fe2+ | contains iron | Cereibacter sphaeroides | |
3.1.1.1 | Mn2+ | contains Mn2+ | Cereibacter sphaeroides | |
3.1.1.1 | Zn2+ | contains Zn2+ | Cereibacter sphaeroides |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.1 | Cereibacter sphaeroides | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.1 | HisTrap HP column chromatography and Superdex 200 gel filtration | Cereibacter sphaeroides |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.1 | 1,4-diacetoxybenzene + H2O | - |
Cereibacter sphaeroides | ? | - |
? | |
3.1.1.1 | 1-naphthyl acetate + H2O | - |
Cereibacter sphaeroides | 1-naphthol + acetate | - |
? | |
3.1.1.1 | 1-naphthyl butyrate + H2O | - |
Cereibacter sphaeroides | 1-naphthol + butyrate | - |
? | |
3.1.1.1 | 2-naphthyl acetate + H2O | - |
Cereibacter sphaeroides | 2-naphthol + acetate | - |
? | |
3.1.1.1 | 2-naphthyl propionate + H2O | - |
Cereibacter sphaeroides | 2-naphthol + propionate | - |
? | |
3.1.1.1 | 2-phenethyl acetate + H2O | - |
Cereibacter sphaeroides | 2-phenylethanol + acetate | - |
? | |
3.1.1.1 | 2-thionaphthyl acetate + H2O | - |
Cereibacter sphaeroides | 2-thionaphthol + acetate | - |
? | |
3.1.1.1 | 3-acetoxypyridine + H2O | - |
Cereibacter sphaeroides | ? | - |
? | |
3.1.1.1 | 4'-chloroacetanilide + H2O | - |
Cereibacter sphaeroides | ? | - |
? | |
3.1.1.1 | 4-hydroxyphenyl acetate + H2O | - |
Cereibacter sphaeroides | 4-hydroxyphenol + acetate | - |
? | |
3.1.1.1 | 4-methylbenzyl acetate + H2O | - |
Cereibacter sphaeroides | 4-methylbenzyl alcohol + acetate | - |
? | |
3.1.1.1 | 4-methylumbelliferyl acetate + H2O | - |
Cereibacter sphaeroides | 4-methylumbelliferone + acetate | - |
? | |
3.1.1.1 | 4-methylumbelliferyl acetate + H2O | - |
Cereibacter sphaeroides | 4-methylumbelliferol + acetate | - |
? | |
3.1.1.1 | 4-methylumbelliferyl butyrate + H2O | - |
Cereibacter sphaeroides | 4-methylumbelliferone + butyrate | - |
? | |
3.1.1.1 | 4-methylumbelliferyl heptanoate + H2O | - |
Cereibacter sphaeroides | 4-methylumbelliferone + heptanoate | - |
? | |
3.1.1.1 | 4-nitroacetanilide + H2O | - |
Cereibacter sphaeroides | ? | - |
? | |
3.1.1.1 | 4-nitrophenyl acetate + H2O | - |
Cereibacter sphaeroides | 4-nitrophenol + acetate | - |
? | |
3.1.1.1 | 4-nitrophenyl butyrate + H2O | - |
Cereibacter sphaeroides | 4-nitrophenol + butyrate | - |
? | |
3.1.1.1 | 4-nitrophenyl butyrate + H2O | best substrate | Cereibacter sphaeroides | 4-nitrophenol + butyrate | - |
? | |
3.1.1.1 | 4-nitrophenyl hexanoate + H2O | - |
Cereibacter sphaeroides | 4-nitrophenol + hexanoate | - |
? | |
3.1.1.1 | 4-nitrophenyl methylphosphonate + H2O | - |
Cereibacter sphaeroides | 4-nitrophenol + methylphosphonate | - |
? | |
3.1.1.1 | 4-nitrophenyl octanoate + H2O | - |
Cereibacter sphaeroides | 4-nitrophenol + octanoate | - |
? | |
3.1.1.1 | benzyl acetate + H2O | - |
Cereibacter sphaeroides | benzyl alcohol + acetate | - |
? | |
3.1.1.1 | cyclohexyl 4-nitrophenyl methylphosphonate + H2O | - |
Cereibacter sphaeroides | ? | - |
? | |
3.1.1.1 | ethyl (4-nitrophenyl)acetate + H2O | - |
Cereibacter sphaeroides | ethanol + (4-nitrophenyl)acetate | - |
? | |
3.1.1.1 | ethyl (naphthalen-2-yl)acetate + H2O | - |
Cereibacter sphaeroides | (naphthalen-2-yl)acetate + ethanol | - |
? | |
3.1.1.1 | ethyl 4-nitrophenyl methylphosphonate + H2O | - |
Cereibacter sphaeroides | ? | - |
? | |
3.1.1.1 | ethyl butyrylacetate + H2O | - |
Cereibacter sphaeroides | ? | - |
? | |
3.1.1.1 | indoxyl acetate + H2O | - |
Cereibacter sphaeroides | indol-3-ol + acetate | - |
? | |
3.1.1.1 | isobutyl 4-nitrophenyl methylphosphonate + H2O | - |
Cereibacter sphaeroides | ? | - |
? | |
3.1.1.1 | isobutyl 4-nitrophenyl methylphosphonate + H2O | - |
Cereibacter sphaeroides | 4-nitrophenol + isobutyl methylphosphonate | - |
? | |
3.1.1.1 | isopropyl 4-nitrophenyl methylphosphonate + H2O | - |
Cereibacter sphaeroides | ? | - |
? | |
3.1.1.1 | methyl paraoxon + H2O | - |
Cereibacter sphaeroides | ? | - |
? | |
3.1.1.1 | paraoxon + H2O | - |
Cereibacter sphaeroides | diethylphosphate + 4-nitrophenol | - |
? | |
3.1.1.1 | phenyl acetate + H2O | - |
Cereibacter sphaeroides | phenol + acetate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.1 | homotetramer | - |
Cereibacter sphaeroides |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.1 | Rsp3690 | - |
Cereibacter sphaeroides |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.1 | 0.063 | - |
4-methylumbelliferyl heptanoate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 0.1 | - |
4'-chloroacetanilide | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 0.36 | - |
4-nitroacetanilide | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 0.5 | - |
4-nitrophenyl octanoate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 0.65 | - |
4-methylumbelliferyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 0.79 | - |
ethyl butyrylacetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 1.3 | - |
paraoxon | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 1.7 | - |
ethyl 2-naphthyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 1.8 | - |
ethyl 4-nitrophenyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 2.1 | - |
methyl paraoxon | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 2.3 | - |
indoxyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 2.5 | - |
Benzyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 3.1 | - |
isopropyl 4-nitrophenyl methylphosphonate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 4.3 | - |
3-acetoxypyridine | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 4.8 | - |
1-naphthyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 4.9 | - |
4-Methylumbelliferyl butyrate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 6.5 | - |
4-nitrophenyl hexanoate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 8 | - |
isobutyl 4-nitrophenyl methylphosphonate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 9 | - |
1-naphthyl butyrate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 30 | - |
phenyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 40 | - |
2-naphthyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 47 | - |
4-methylbenzyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 50 | - |
4-nitrophenyl butyrate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 83 | - |
2-naphthyl propionate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 84 | - |
ethyl 4-nitrophenyl methylphosphonate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 108 | - |
1,4-diacetoxybenzene | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 120 | - |
4-nitrophenyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.1 | 0.063 | - |
4'-chloroacetanilide | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 0.21 | - |
cyclohexyl 4-nitrophenyl methylphosphonate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 0.25 | - |
2-phenethyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 0.47 | - |
2-thionaphthyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 0.55 | - |
4-nitroacetanilide | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 1.2 | - |
indoxyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 2.4 | - |
3-acetoxypyridine | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 2.4 | - |
ethyl butyrylacetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 2.6 | - |
4-methylumbelliferyl heptanoate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 2.6 | - |
isopropyl 4-nitrophenyl methylphosphonate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 2.7 | - |
4-methylumbelliferyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 2.8 | - |
ethyl 2-naphthyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 4.3 | - |
ethyl 4-nitrophenyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 5 | - |
methyl paraoxon | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 7.5 | - |
1-naphthyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 11 | - |
phenyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 11 | - |
isobutyl 4-nitrophenyl methylphosphonate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 12 | - |
paraoxon | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 15 | - |
4-nitrophenyl octanoate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 31 | - |
4-Methylumbelliferyl butyrate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 38 | - |
4-methylbenzyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 100 | - |
2-naphthyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 130 | - |
4-nitrophenyl hexanoate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 130 | - |
1,4-diacetoxybenzene | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 150 | - |
2-naphthyl propionate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 160 | - |
Benzyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 230 | - |
4-nitrophenyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 380 | - |
ethyl 4-nitrophenyl methylphosphonate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 500 | - |
1-naphthyl butyrate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1.1.1 | 880 | - |
4-nitrophenyl butyrate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides |