EC Number | Cloned (Comment) | Organism |
---|---|---|
4.3.1.7 | expressed in Escherichia coli as a His-tagged fusion protein | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.3.1.7 | D362A | mutant shows highly decreased kcat compared to wild-type, mutant undergoes quicker inactivation compared to wild-type | Escherichia coli |
4.3.1.7 | D362E | mutant shows highly decreased kcat compared to wild-type | Escherichia coli |
4.3.1.7 | D362N | mutant shows highly decreased kcat compared to wild-type | Escherichia coli |
4.3.1.7 | E287A | mutant shows highly decreased kcat compared to wild-type, mutant undergoes quicker inactivation compared to wild-type | Escherichia coli |
4.3.1.7 | E287D | mutant shows highly decreased kcat compared to wild-type | Escherichia coli |
4.3.1.7 | E287H | mutant shows highly decreased kcat compared to wild-type | Escherichia coli |
4.3.1.7 | E287Q | mutant shows highly decreased kcat compared to wild-type | Escherichia coli |
4.3.1.7 | N193A | mutant shows highly decreased kcat compared to wild-type, mutant undergoes quicker inactivation compared to wild-type | Escherichia coli |
4.3.1.7 | N193D | mutant shows decreased kcat compared to wild-type but mutant retains partial activity, Km (ethanolamine) increased compared to wild-type | Escherichia coli |
4.3.1.7 | Q162A | mutant shows highly decreased kcat compared to wild-type, mutant undergoes quicker inactivation compared to wild-type | Escherichia coli |
4.3.1.7 | Q162E | mutant shows decreased kcat compared to wild-type but mutant retains partial activity, Km (ethanolamine) increased compared to wild-type | Escherichia coli |
4.3.1.7 | Q162H | mutant shows decreased kcat compared to wild-type but mutant retains partial activity, Km (ethanolamine) increased compared to wild-type | Escherichia coli |
4.3.1.7 | Q162K | mutant shows highly decreased kcat compared to wild-type | Escherichia coli |
4.3.1.7 | R160A | mutant shows highly decreased kcat compared to wild-type, mutant undergoes quicker inactivation compared to wild-type | Escherichia coli |
4.3.1.7 | R160K | mutant shows decreased kcat compared to wild-type but mutant retains partial activity, Km (ethanolamine) increased compared to wild-type | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.3.1.7 | 0.0033 | - |
ethanolamine | run 2, pH 8, 37°C, wild-type | Escherichia coli | |
4.3.1.7 | 0.21 | - |
ethanolamine | run 2, pH 8, 37°C, mutant N193D | Escherichia coli | |
4.3.1.7 | 0.21 | - |
ethanolamine | run 2, pH 8, 37°C, mutant R160K | Escherichia coli | |
4.3.1.7 | 0.22 | - |
ethanolamine | run 2, pH 8, 37°C, mutant Q162H | Escherichia coli | |
4.3.1.7 | 0.73 | - |
ethanolamine | run 2, pH 8, 37°C, mutant Q162E | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.3.1.7 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.3.1.7 | using Ni-NTA chromatography | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.3.1.7 | ethanolamine | - |
Escherichia coli | acetaldehyde + NH3 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.3.1.7 | EAL | - |
Escherichia coli |
4.3.1.7 | ethanolamine ammonia-lyase | - |
Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.3.1.7 | 37 | - |
assay at | Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.3.1.7 | 0.036 | - |
ethanolamine | run 1, pH 8, 37°C, mutant E287A | Escherichia coli | |
4.3.1.7 | 0.038 | - |
ethanolamine | run 2, pH 8, 37°C, mutant D362N | Escherichia coli | |
4.3.1.7 | 0.051 | - |
ethanolamine | run 2, pH 8, 37°C, mutant Q162K | Escherichia coli | |
4.3.1.7 | 0.066 | - |
ethanolamine | run 2, pH 8, 37°C, mutant E287H | Escherichia coli | |
4.3.1.7 | 0.085 | - |
ethanolamine | run 1, pH 8, 37°C, mutant D362A | Escherichia coli | |
4.3.1.7 | 0.086 | - |
ethanolamine | run 2, pH 8, 37°C, mutant E287Q | Escherichia coli | |
4.3.1.7 | 0.67 | - |
ethanolamine | run 2, pH 8, 37°C, mutant D362E | Escherichia coli | |
4.3.1.7 | 1 | - |
ethanolamine | run 1, pH 8, 37°C, mutant R160A | Escherichia coli | |
4.3.1.7 | 1.3 | - |
ethanolamine | run 1, pH 8, 37°C, mutant N193A | Escherichia coli | |
4.3.1.7 | 2.1 | - |
ethanolamine | run 2, pH 8, 37°C, mutant E287D | Escherichia coli | |
4.3.1.7 | 3 | 6 | ethanolamine | run 2, pH 8, 37°C, mutant N193D | Escherichia coli | |
4.3.1.7 | 6 | - |
ethanolamine | run 2, pH 8, 37°C, mutant Q162H | Escherichia coli | |
4.3.1.7 | 20 | - |
ethanolamine | run 1, pH 8, 37°C, mutant Q162A | Escherichia coli | |
4.3.1.7 | 79 | - |
ethanolamine | run 2, pH 8, 37°C, mutant R160K | Escherichia coli | |
4.3.1.7 | 110 | - |
ethanolamine | run 2, pH 8, 37°C, mutant Q162E | Escherichia coli | |
4.3.1.7 | 440 | - |
ethanolamine | run 2, pH 8, 37°C, wild-type | Escherichia coli | |
4.3.1.7 | 770 | - |
ethanolamine | run 1, pH 8, 37°C, wild-type | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.3.1.7 | 8 | - |
assay at | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.3.1.7 | adenosylcobalamin | - |
Escherichia coli |