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Literature summary extracted from

  • Skovpen, Y.V.; Palmer, D.R.
    Dihydrodipicolinate synthase from Campylobacter jejuni: kinetic mechanism of cooperative allosteric inhibition and inhibitor-induced substrate cooperativity (2013), Biochemistry, 52, 5454-5462.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.3.7 gene dapA, expression of His-tagged enzyme in Escherichia coli strain XL1-Blue Campylobacter jejuni

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.3.7 (S)-lysine feedback inhibition, the tetrameric enzyme has two allosteric sites, each of which binds two molecules of lysine. Lysine binds highly cooperatively, and primarily to the F form of the enzyme during the ping-pong mechanism. (S)-Lysine is an uncompetitive partial inhibitor with respect to its first substrate, pyruvate, and a mixed partial inhibitor with respect to its second substrate, (S)-aspartate-4-semialdehyde Campylobacter jejuni

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.3.7 additional information
-
 additional information kinetic modeling Campylobacter jejuni
4.3.3.7 2.83
-
 pyruvate pH and temperature not specified in the publication Campylobacter jejuni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.3.7 (S)-aspartate-4-semialdehyde + pyruvate Campylobacter jejuni
-
 (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.3.7 Campylobacter jejuni
-
 gene dapA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.3.7 recombinant His-tagged enzyme from Escherichia coli strain XL1-Blue by nickel affinity chromatography Campylobacter jejuni

Reaction

EC Number Reaction Comment Organism Reaction ID
4.3.3.7 pyruvate + L-aspartate-4-semialdehyde = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O ping-pong kinetic reaction mechanism Campylobacter jejuni
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.3.7 (S)-aspartate-4-semialdehyde + pyruvate
-
 Campylobacter jejuni (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O
-
 ?
4.3.3.7 (S)-aspartate-4-semialdehyde + pyruvate (S)-aspartate-4-semialdehyde binds cooperatively in the presence of (S)-lysine with an average cooperativity coefficient n = 1.3, and the cooperativity of binding increases at near-KM concentrations of pyruvate with an average cooperativity coefficient n = 1.0 Campylobacter jejuni (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.3.7 homotetramer
-
 Campylobacter jejuni

Synonyms

EC Number Synonyms Comment Organism
4.3.3.7 DHDPS
-
 Campylobacter jejuni
4.3.3.7 dihydrodipicolinate synthase
-
 Campylobacter jejuni

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.3.3.7 additional information
-
 additional information (S)-lysine allosteric inhibition kinetics and mechanism, overview Campylobacter jejuni

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.3.3.7 0.065
-
 pH and temperature not specified in the publication Campylobacter jejuni (S)-lysine