EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.3.45 | expressed in Escherichia coli BL21(DE3) cells | Bacteroides thetaiotaomicron |
3.1.3.45 | expressed in Escherichia coli BL21(DE3) cells | Haemophilus influenzae |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.1.3.45 | enzyme bound to Mg2+ and metavanadate, hanging drop vapor diffusion method, using 30% (w/v) polyethylene glycol MME 550, 40 mM MgCl2 and 100 mM HEPES (pH 7.5), at 18°C | Bacteroides thetaiotaomicron |
3.1.3.45 | enzyme bound to Mg2+ and metavanadate, hanging drop vapor diffusion method, using 30% (w/v) polyethylene glycol MME 550, 40 mM MgCl2 and 100 mM HEPES (pH 7.5), at 18°C | Haemophilus influenzae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.45 | 0.0001 | - |
2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate | at pH 7.0 and 25°C | Bacteroides thetaiotaomicron | |
3.1.3.45 | 0.0009 | - |
KDN 9-phosphate | at pH 7.0 and 25°C | Bacteroides thetaiotaomicron | |
3.1.3.45 | 0.16 | - |
2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate | at pH 7.0 and 25°C | Haemophilus influenzae | |
3.1.3.45 | 1.6 | - |
KDN 9-phosphate | at pH 7.0 and 25°C | Haemophilus influenzae |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.45 | Mg2+ | required | Bacteroides thetaiotaomicron | |
3.1.3.45 | Mg2+ | required | Haemophilus influenzae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.45 | 2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate + H2O | Bacteroides thetaiotaomicron | best substrate | ? + phosphate | - |
? | |
3.1.3.45 | 2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate + H2O | Haemophilus influenzae | best substrate | ? + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.45 | Bacteroides thetaiotaomicron | - |
- |
- |
3.1.3.45 | Haemophilus influenzae | P45314 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.3.45 | TALON metal affinity resin column chromatography | Bacteroides thetaiotaomicron |
3.1.3.45 | TALON metal affinity resin column chromatography | Haemophilus influenzae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.45 | 2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate + H2O | - |
Bacteroides thetaiotaomicron | ? + phosphate | - |
? | |
3.1.3.45 | 2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate + H2O | - |
Haemophilus influenzae | ? + phosphate | - |
? | |
3.1.3.45 | 2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate + H2O | best substrate | Bacteroides thetaiotaomicron | ? + phosphate | - |
? | |
3.1.3.45 | 2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate + H2O | best substrate | Haemophilus influenzae | ? + phosphate | - |
? | |
3.1.3.45 | KDN 9-phosphate + H2O | - |
Bacteroides thetaiotaomicron | 3-deoxy-D-glycero-beta-D-galacto-non-2-ulopyranosonate + phosphate | - |
? | |
3.1.3.45 | KDN 9-phosphate + H2O | - |
Haemophilus influenzae | 3-deoxy-D-glycero-beta-D-galacto-non-2-ulopyranosonate + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.3.45 | dimer | x-ray crystallography | Bacteroides thetaiotaomicron |
3.1.3.45 | dimer | x-ray crystallography | Haemophilus influenzae |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.3.45 | 2-keto-3-deoxy-D-manno-octulosonate 8-phosphate phosphohydrolase | - |
Bacteroides thetaiotaomicron |
3.1.3.45 | 2-keto-3-deoxy-D-manno-octulosonate 8-phosphate phosphohydrolase | - |
Haemophilus influenzae |
3.1.3.45 | KDO8P phosphatase | - |
Bacteroides thetaiotaomicron |
3.1.3.45 | KDO8P phosphatase | - |
Haemophilus influenzae |
3.1.3.45 | KDO8PP | - |
Bacteroides thetaiotaomicron |
3.1.3.45 | KDO8PP | - |
Haemophilus influenzae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.45 | 0.021 | - |
KDN 9-phosphate | at pH 7.0 and 25°C | Haemophilus influenzae | |
3.1.3.45 | 0.061 | - |
KDN 9-phosphate | at pH 7.0 and 25°C | Bacteroides thetaiotaomicron | |
3.1.3.45 | 0.78 | - |
2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate | at pH 7.0 and 25°C | Haemophilus influenzae | |
3.1.3.45 | 1.5 | - |
2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate | at pH 7.0 and 25°C | Bacteroides thetaiotaomicron |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.45 | 0.013 | - |
KDN 9-phosphate | at pH 7.0 and 25°C | Haemophilus influenzae | |
3.1.3.45 | 0.069 | - |
KDN 9-phosphate | at pH 7.0 and 25°C | Bacteroides thetaiotaomicron | |
3.1.3.45 | 4.8 | - |
2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate | at pH 7.0 and 25°C | Haemophilus influenzae | |
3.1.3.45 | 15 | - |
2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate | at pH 7.0 and 25°C | Bacteroides thetaiotaomicron |