EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.3.15 | expressed in Escherichia coli BL21(DE3) cells | Lactococcus lactis subsp. lactis |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.1.3.15 | sitting drop vapor diffusion method, using 25% (w/v) PEG 4000, 0.1 M sodium acetate (pH 4.6), and 0.2 M ammonium sulfate | Lactococcus lactis subsp. lactis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.3.15 | D17N | inactive | Lactococcus lactis subsp. lactis |
3.1.3.15 | D228N | the mutant shows reduced catalytic efficiency and kcat is reduced by approximately 6000fold compared to the wild type enzyme | Lactococcus lactis subsp. lactis |
3.1.3.15 | E115Q | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Lactococcus lactis subsp. lactis |
3.1.3.15 | H230N | inactive | Lactococcus lactis subsp. lactis |
3.1.3.15 | H42N | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Lactococcus lactis subsp. lactis |
3.1.3.15 | R160A | inactive | Lactococcus lactis subsp. lactis |
3.1.3.15 | R197M | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Lactococcus lactis subsp. lactis |
3.1.3.15 | Y117A | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Lactococcus lactis subsp. lactis |
3.1.3.15 | Y117F | the mutant shows increased catalytic efficiency compared to the wild type enzyme | Lactococcus lactis subsp. lactis |
3.1.3.15 | Y157F | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Lactococcus lactis subsp. lactis |
3.1.3.15 | Y161A | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Lactococcus lactis subsp. lactis |
3.1.3.15 | Y161F | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Lactococcus lactis subsp. lactis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.15 | 0.14 | - |
L-histidinol phosphate | mutant enzyme D228N, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
3.1.3.15 | 0.74 | - |
L-histidinol phosphate | mutant enzyme Y117F, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
3.1.3.15 | 1.3 | - |
L-histidinol phosphate | mutant enzyme H42N, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
3.1.3.15 | 1.5 | - |
L-histidinol phosphate | mutant enzyme R160M, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
3.1.3.15 | 1.7 | - |
L-histidinol phosphate | mutant enzyme E115Q, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
3.1.3.15 | 1.7 | - |
L-histidinol phosphate | mutant enzyme HY157F, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
3.1.3.15 | 1.9 | - |
L-histidinol phosphate | wild type enzyme, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
3.1.3.15 | 2.3 | - |
L-histidinol phosphate | mutant enzyme Y161F, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
3.1.3.15 | 2.5 | - |
L-histidinol phosphate | mutant enzyme Y161A, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
3.1.3.15 | 4.5 | - |
L-histidinol phosphate | mutant enzyme R197M, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
3.1.3.15 | 5.4 | - |
L-histidinol phosphate | mutant enzyme Y117A, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.15 | Iron | contains iron in the active site | Lactococcus lactis subsp. lactis | |
3.1.3.15 | additional information | the enzyme does not show any significant increases in catalytic activity when Zn2+, Mn2+, Co2+ or Ni2+ are added | Lactococcus lactis subsp. lactis | |
3.1.3.15 | Zn2+ | contains Zn2+ in the active site | Lactococcus lactis subsp. lactis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.15 | L-histidinol phosphate + H2O | Lactococcus lactis subsp. lactis | - |
L-histidinol + phosphate | - |
? | |
3.1.3.15 | L-histidinol phosphate + H2O | Lactococcus lactis subsp. lactis IL1403 | - |
L-histidinol + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.15 | Lactococcus lactis subsp. lactis | Q02150 | - |
- |
3.1.3.15 | Lactococcus lactis subsp. lactis IL1403 | Q02150 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.3.15 | Ni-NTA affinity chromatography | Lactococcus lactis subsp. lactis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.15 | L-histidinol phosphate + H2O | - |
Lactococcus lactis subsp. lactis | L-histidinol + phosphate | - |
? | |
3.1.3.15 | L-histidinol phosphate + H2O | - |
Lactococcus lactis subsp. lactis IL1403 | L-histidinol + phosphate | - |
? | |
3.1.3.15 | N-formyl-L-histidinol phosphate + H2O | the rate of hydrolysis of N-formyl-L-histidinol phosphate is less than 1% of the rate of hydrolysis of L-histidinol phosphate at pH 8.5 | Lactococcus lactis subsp. lactis | N-formyl-L-histidinol + phosphate | - |
? | |
3.1.3.15 | N-formyl-L-histidinol phosphate + H2O | the rate of hydrolysis of N-formyl-L-histidinol phosphate is less than 1% of the rate of hydrolysis of L-histidinol phosphate at pH 8.5 | Lactococcus lactis subsp. lactis IL1403 | N-formyl-L-histidinol + phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.3.15 | HPP | - |
Lactococcus lactis subsp. lactis |
3.1.3.15 | L-histidinol phosphate phosphatase | - |
Lactococcus lactis subsp. lactis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.15 | 0.031 | - |
L-histidinol phosphate | mutant enzyme D228N, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
3.1.3.15 | 0.62 | - |
L-histidinol phosphate | mutant enzyme H42N, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
3.1.3.15 | 0.74 | - |
L-histidinol phosphate | mutant enzyme R160M, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
3.1.3.15 | 31 | - |
L-histidinol phosphate | mutant enzyme E115Q, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
3.1.3.15 | 38 | - |
L-histidinol phosphate | mutant enzyme R197M, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
3.1.3.15 | 48 | - |
L-histidinol phosphate | mutant enzyme Y161A, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
3.1.3.15 | 53 | - |
L-histidinol phosphate | mutant enzyme Y117A, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
3.1.3.15 | 61 | - |
L-histidinol phosphate | mutant enzyme HY157F, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
3.1.3.15 | 110 | - |
L-histidinol phosphate | mutant enzyme Y117F, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
3.1.3.15 | 174 | - |
L-histidinol phosphate | wild type enzyme, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
3.1.3.15 | 180 | - |
L-histidinol phosphate | mutant enzyme Y161F, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.15 | 0.22 | - |
L-histidinol phosphate | mutant enzyme D228N, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
3.1.3.15 | 0.48 | - |
L-histidinol phosphate | mutant enzyme H42N, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
3.1.3.15 | 0.49 | - |
L-histidinol phosphate | mutant enzyme R160M, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
3.1.3.15 | 8.5 | - |
L-histidinol phosphate | mutant enzyme R197M, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
3.1.3.15 | 9.8 | - |
L-histidinol phosphate | mutant enzyme Y117A, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
3.1.3.15 | 18 | - |
L-histidinol phosphate | mutant enzyme E115Q, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
3.1.3.15 | 25 | - |
L-histidinol phosphate | mutant enzyme Y161A, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
3.1.3.15 | 36 | - |
L-histidinol phosphate | mutant enzyme HY157F, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
3.1.3.15 | 79 | - |
L-histidinol phosphate | mutant enzyme Y161F, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
3.1.3.15 | 89 | - |
L-histidinol phosphate | at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
3.1.3.15 | 150 | - |
L-histidinol phosphate | mutant enzyme Y117F, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis |