EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.99.21 | C7A | mutant has a reduced catalytic free energy of activation of up to 0.17 kcal/mol | Pseudomonas aeruginosa |
4.2.99.21 | C7A/K42C | mutant has a reduced catalytic free energy of activation of up to 4.2 kcal/mol. Treatment with bromoethylamine leads to 64% recovery of activity | Pseudomonas aeruginosa |
4.2.99.21 | K42A | mutation leads to the recovery in catalytic free energy of activation of 2.52.7 kcal/mol compared to mutant K42C. Exogenous addition of ethylamine to the K42A variant leads to a neglible recovery of activity, whereas addition of propylamine causes an additional modest loss in catalytic power | Pseudomonas aeruginosa |
4.2.99.21 | K42C | mutant has a reduced catalytic free energy of activation of up to 4.4 kcal/mol. Treatment with bromoethylamine leads to 55% recovery of activity | Pseudomonas aeruginosa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.99.21 | 0.0011 | - |
isochorismate | wild-type, pH 8.0, 20°C | Pseudomonas aeruginosa | |
4.2.99.21 | 0.0021 | - |
isochorismate | mutant C7A, pH 8.0, 20°C | Pseudomonas aeruginosa | |
4.2.99.21 | 0.0156 | - |
isochorismate | mutant C7A/K42C, treatment with bromoethylamine, pH 8.0, 20°C | Pseudomonas aeruginosa | |
4.2.99.21 | 0.023 | - |
isochorismate | mutant K42C, treatment with bromoethylamine, pH 8.0, 20°C | Pseudomonas aeruginosa | |
4.2.99.21 | 0.03 | - |
isochorismate | mutant K42A, pH 8.0, 20°C | Pseudomonas aeruginosa | |
4.2.99.21 | 0.048 | - |
isochorismate | mutant K42A, presence of propylamine, pH 8.0, 20°C | Pseudomonas aeruginosa | |
4.2.99.21 | 0.06 | - |
isochorismate | mutant K42A, presence of ethylamine, pH 8.0, 20°C | Pseudomonas aeruginosa | |
4.2.99.21 | 0.114 | - |
isochorismate | mutant C7A/K42C, treatment with bromoethanol, pH 8.0, 20°C | Pseudomonas aeruginosa | |
4.2.99.21 | 0.123 | - |
isochorismate | mutant C7A/K42C, pH 8.0, 20°C | Pseudomonas aeruginosa | |
4.2.99.21 | 0.125 | - |
isochorismate | mutant K42C, treatment with bromoethanol, pH 8.0, 20°C | Pseudomonas aeruginosa | |
4.2.99.21 | 0.134 | - |
isochorismate | mutant K42C, pH 8.0, 20°C | Pseudomonas aeruginosa |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.99.21 | Pseudomonas aeruginosa | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
4.2.99.21 | isochorismate = salicylate + pyruvate | the lysine42 residue is required in a specific conformation to stabilize the transition state | Pseudomonas aeruginosa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.99.21 | isochorismate | - |
Pseudomonas aeruginosa | salicylate + pyruvate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.99.21 | PchB | - |
Pseudomonas aeruginosa |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.99.21 | 0.0083 | - |
isochorismate | mutant K42C, pH 8.0, 20°C | Pseudomonas aeruginosa | |
4.2.99.21 | 0.0089 | - |
isochorismate | mutant C7A/K42C, pH 8.0, 20°C | Pseudomonas aeruginosa | |
4.2.99.21 | 0.0128 | - |
isochorismate | mutant C7A/K42C, treatment with bromoethanol, pH 8.0, 20°C | Pseudomonas aeruginosa | |
4.2.99.21 | 0.0184 | - |
isochorismate | mutant K42C, treatment with bromoethanol, pH 8.0, 20°C | Pseudomonas aeruginosa | |
4.2.99.21 | 0.045 | - |
isochorismate | mutant K42A, pH 8.0, 20°C | Pseudomonas aeruginosa | |
4.2.99.21 | 0.083 | - |
isochorismate | mutant K42A, presence of ethylamine, pH 8.0, 20°C | Pseudomonas aeruginosa | |
4.2.99.21 | 0.0945 | - |
isochorismate | mutant K42C, treatment with bromoethylamine, pH 8.0, 20°C | Pseudomonas aeruginosa | |
4.2.99.21 | 0.098 | - |
isochorismate | mutant K42A, presence of propylamine, pH 8.0, 20°C | Pseudomonas aeruginosa | |
4.2.99.21 | 0.105 | - |
isochorismate | mutant C7A/K42C, treatment with bromoethylamine, pH 8.0, 20°C | Pseudomonas aeruginosa | |
4.2.99.21 | 0.13 | - |
isochorismate | wild-type, pH 8.0, 20°C | Pseudomonas aeruginosa | |
4.2.99.21 | 0.2 | - |
isochorismate | mutant C7A, pH 8.0, 20°C | Pseudomonas aeruginosa |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.99.21 | 0.062 | - |
isochorismate | mutant K42C, pH 8.0, 20°C | Pseudomonas aeruginosa | |
4.2.99.21 | 0.065 | - |
isochorismate | mutant C7A/K42C, pH 8.0, 20°C | Pseudomonas aeruginosa | |
4.2.99.21 | 0.103 | - |
isochorismate | mutant C7A/K42C, treatment with bromoethanol, pH 8.0, 20°C | Pseudomonas aeruginosa | |
4.2.99.21 | 0.147 | - |
isochorismate | mutant K42C, treatment with bromoethanol, pH 8.0, 20°C | Pseudomonas aeruginosa | |
4.2.99.21 | 1.37 | - |
isochorismate | mutant K42A, presence of propylamine, pH 8.0, 20°C | Pseudomonas aeruginosa | |
4.2.99.21 | 1.5 | - |
isochorismate | mutant K42A, pH 8.0, 20°C | Pseudomonas aeruginosa | |
4.2.99.21 | 2.06 | - |
isochorismate | mutant K42A, presence of ethylamine, pH 8.0, 20°C | Pseudomonas aeruginosa | |
4.2.99.21 | 4.2 | - |
isochorismate | mutant K42C, treatment with bromoethylamine, pH 8.0, 20°C | Pseudomonas aeruginosa | |
4.2.99.21 | 6.7 | - |
isochorismate | mutant C7A/K42C, treatment with bromoethylamine, pH 8.0, 20°C | Pseudomonas aeruginosa | |
4.2.99.21 | 93 | - |
isochorismate | mutant C7A, pH 8.0, 20°C | Pseudomonas aeruginosa | |
4.2.99.21 | 124 | - |
isochorismate | wild-type, pH 8.0, 20°C | Pseudomonas aeruginosa |