Any feedback?
Literature summary extracted from
- Nicotinamidase from the thermophilic archaeon Acidilobus saccharovorans: structural and functional characteristics (2014), Biochemistry (Moscow), 79, 54-61.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.19 | 2-mercaptoethanol | 2 mM, 2fold activation | Acidilobus saccharovorans |  |
| 3.5.1.19 | dithiothreitol | 2 mM, 2.1fold activation | Acidilobus saccharovorans | |
| 3.5.1.19 | EDTA | 57% inhibitioin at 10 mM. 10% inhibition at 1 mM | Acidilobus saccharovorans | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.1.19 | expression in Escherichia coli | Acidilobus saccharovorans |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.19 | Fe3+ | 1 mM, 94% inhibition | Acidilobus saccharovorans | |
| 3.5.1.19 | NaCl | 50 mM, 12% inhibition | Acidilobus saccharovorans | |
| 3.5.1.19 | Zn2+ | 5 mM, complete inhibition | Acidilobus saccharovorans | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.19 | 0.18 | - |
nicotinamide | pH 7.5, 60°C | Acidilobus saccharovorans | |
| 3.5.1.19 | 0.41 | - |
Pyrazinamide | pH 7.5, 60°C | Acidilobus saccharovorans | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.19 | Fe2+ | the enzyme contains Fe2+ (0.77 mol per mol of protein). 1.6fold activation by 5 mM Fe2+ | Acidilobus saccharovorans | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.19 | 46400 | - |
- |
Acidilobus saccharovorans |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.19 | Acidilobus saccharovorans | D9Q1R5 | - |
- |
| 3.5.1.19 | Acidilobus saccharovorans DSM 16705 | D9Q1R5 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.19 | additional information | no activity with N-carbamoylsarcosine, hydantoic acid, acrylamide, D- and L-asparagine, D- and L-glutamine | Acidilobus saccharovorans | ? | - |
? | |
| 3.5.1.19 | additional information | no activity with N-carbamoylsarcosine, hydantoic acid, acrylamide, D- and L-asparagine, D- and L-glutamine | Acidilobus saccharovorans DSM 16705 | ? | - |
? | |
| 3.5.1.19 | nicotinamide + H2O | - |
Acidilobus saccharovorans | nicotinate + NH3 | - |
? | |
| 3.5.1.19 | nicotinamide + H2O | - |
Acidilobus saccharovorans DSM 16705 | nicotinate + NH3 | - |
? | |
| 3.5.1.19 | pyrazinamide + H2O | - |
Acidilobus saccharovorans | pyrazine-2-carboxylic acid + NH3 | - |
? | |
| 3.5.1.19 | pyrazinamide + H2O | - |
Acidilobus saccharovorans DSM 16705 | pyrazine-2-carboxylic acid + NH3 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.1.19 | homodimer | in solution the protein exists as a dimer with a small admixture of tetrameric form | Acidilobus saccharovorans |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.1.19 | ASAC_0847 | - |
Acidilobus saccharovorans |
| 3.5.1.19 | NA_As0847 | - |
Acidilobus saccharovorans |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.19 | 90 | - |
- |
Acidilobus saccharovorans |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.19 | 60 | 100 | 60°C: about 45% of maximal activity, 100°C: 85% of maximal activity | Acidilobus saccharovorans |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.19 | 60 | - |
t1/2: 180 min | Acidilobus saccharovorans |
| 3.5.1.19 | 80 | - |
t1/2: 35 min | Acidilobus saccharovorans |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.19 | 76.9 | - |
nicotinamide | pH 7.5, 60°C | Acidilobus saccharovorans | |
| 3.5.1.19 | 135.7 | - |
Pyrazinamide | pH 7.5, 60°C | Acidilobus saccharovorans | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.19 | 7.5 | - |
- |
Acidilobus saccharovorans |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.19 | 6.5 | 10 | hydrolysis of nicotinamide of a wide range of pH values | Acidilobus saccharovorans |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.19 | 331 | - |
Pyrazinamide | pH 7.5, 60°C | Acidilobus saccharovorans | |
| 3.5.1.19 | 427 | - |
nicotinamide | pH 7.5, 60°C | Acidilobus saccharovorans | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
