BRENDA - Enzyme Database show

Nicotinamidase from the thermophilic archaeon Acidilobus saccharovorans: structural and functional characteristics

Stekhanova, T.N.; Bezsudnova, E.Y.; Mardanov, A.V.; Osipov, E.M.; Ravin, N.V.; Skryabin, K.G.; Popov, V.O.; Biochemistry (Moscow) 79, 54-61 (2014)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.1.19
2-mercaptoethanol
2 mM, 2fold activation
Acidilobus saccharovorans
3.5.1.19
dithiothreitol
2 mM, 2.1fold activation
Acidilobus saccharovorans
3.5.1.19
EDTA
57% inhibitioin at 10 mM. 10% inhibition at 1 mM
Acidilobus saccharovorans
Cloned(Commentary)
EC Number
Commentary
Organism
3.5.1.19
expression in Escherichia coli
Acidilobus saccharovorans
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.19
Fe3+
1 mM, 94% inhibition
Acidilobus saccharovorans
3.5.1.19
NaCl
50 mM, 12% inhibition
Acidilobus saccharovorans
3.5.1.19
Zn2+
5 mM, complete inhibition
Acidilobus saccharovorans
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.19
0.18
-
nicotinamide
pH 7.5, 60°C
Acidilobus saccharovorans
3.5.1.19
0.41
-
Pyrazinamide
pH 7.5, 60°C
Acidilobus saccharovorans
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.1.19
Fe2+
the enzyme contains Fe2+ (0.77 mol per mol of protein). 1.6fold activation by 5 mM Fe2+
Acidilobus saccharovorans
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.19
46400
-
-
Acidilobus saccharovorans
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.1.19
Acidilobus saccharovorans
D9Q1R5
-
-
3.5.1.19
Acidilobus saccharovorans DSM 16705
D9Q1R5
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.19
additional information
no activity with N-carbamoylsarcosine, hydantoic acid, acrylamide, D- and L-asparagine, D- and L-glutamine
729184
Acidilobus saccharovorans
?
-
-
-
-
3.5.1.19
additional information
no activity with N-carbamoylsarcosine, hydantoic acid, acrylamide, D- and L-asparagine, D- and L-glutamine
729184
Acidilobus saccharovorans DSM 16705
?
-
-
-
-
3.5.1.19
nicotinamide + H2O
-
729184
Acidilobus saccharovorans
nicotinate + NH3
-
-
-
?
3.5.1.19
nicotinamide + H2O
-
729184
Acidilobus saccharovorans DSM 16705
nicotinate + NH3
-
-
-
?
3.5.1.19
pyrazinamide + H2O
-
729184
Acidilobus saccharovorans
pyrazine-2-carboxylic acid + NH3
-
-
-
?
3.5.1.19
pyrazinamide + H2O
-
729184
Acidilobus saccharovorans DSM 16705
pyrazine-2-carboxylic acid + NH3
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.1.19
homodimer
in solution the protein exists as a dimer with a small admixture of tetrameric form
Acidilobus saccharovorans
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.1.19
90
-
-
Acidilobus saccharovorans
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
3.5.1.19
60
100
60°C: about 45% of maximal activity, 100°C: 85% of maximal activity
Acidilobus saccharovorans
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.1.19
60
-
t1/2: 180 min
Acidilobus saccharovorans
3.5.1.19
80
-
t1/2: 35 min
Acidilobus saccharovorans
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.5.1.19
76.9
-
nicotinamide
pH 7.5, 60°C
Acidilobus saccharovorans
3.5.1.19
135.7
-
Pyrazinamide
pH 7.5, 60°C
Acidilobus saccharovorans
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.19
7.5
-
-
Acidilobus saccharovorans
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.1.19
6.5
10
hydrolysis of nicotinamide of a wide range of pH values
Acidilobus saccharovorans
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.1.19
2-mercaptoethanol
2 mM, 2fold activation
Acidilobus saccharovorans
3.5.1.19
dithiothreitol
2 mM, 2.1fold activation
Acidilobus saccharovorans
3.5.1.19
EDTA
57% inhibitioin at 10 mM. 10% inhibition at 1 mM
Acidilobus saccharovorans
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.19
expression in Escherichia coli
Acidilobus saccharovorans
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.19
Fe3+
1 mM, 94% inhibition
Acidilobus saccharovorans
3.5.1.19
NaCl
50 mM, 12% inhibition
Acidilobus saccharovorans
3.5.1.19
Zn2+
5 mM, complete inhibition
Acidilobus saccharovorans
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.19
0.18
-
nicotinamide
pH 7.5, 60°C
Acidilobus saccharovorans
3.5.1.19
0.41
-
Pyrazinamide
pH 7.5, 60°C
Acidilobus saccharovorans
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.1.19
Fe2+
the enzyme contains Fe2+ (0.77 mol per mol of protein). 1.6fold activation by 5 mM Fe2+
Acidilobus saccharovorans
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.19
46400
-
-
Acidilobus saccharovorans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.19
additional information
no activity with N-carbamoylsarcosine, hydantoic acid, acrylamide, D- and L-asparagine, D- and L-glutamine
729184
Acidilobus saccharovorans
?
-
-
-
-
3.5.1.19
additional information
no activity with N-carbamoylsarcosine, hydantoic acid, acrylamide, D- and L-asparagine, D- and L-glutamine
729184
Acidilobus saccharovorans DSM 16705
?
-
-
-
-
3.5.1.19
nicotinamide + H2O
-
729184
Acidilobus saccharovorans
nicotinate + NH3
-
-
-
?
3.5.1.19
nicotinamide + H2O
-
729184
Acidilobus saccharovorans DSM 16705
nicotinate + NH3
-
-
-
?
3.5.1.19
pyrazinamide + H2O
-
729184
Acidilobus saccharovorans
pyrazine-2-carboxylic acid + NH3
-
-
-
?
3.5.1.19
pyrazinamide + H2O
-
729184
Acidilobus saccharovorans DSM 16705
pyrazine-2-carboxylic acid + NH3
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.1.19
homodimer
in solution the protein exists as a dimer with a small admixture of tetrameric form
Acidilobus saccharovorans
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.1.19
90
-
-
Acidilobus saccharovorans
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
3.5.1.19
60
100
60°C: about 45% of maximal activity, 100°C: 85% of maximal activity
Acidilobus saccharovorans
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.1.19
60
-
t1/2: 180 min
Acidilobus saccharovorans
3.5.1.19
80
-
t1/2: 35 min
Acidilobus saccharovorans
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.5.1.19
76.9
-
nicotinamide
pH 7.5, 60°C
Acidilobus saccharovorans
3.5.1.19
135.7
-
Pyrazinamide
pH 7.5, 60°C
Acidilobus saccharovorans
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.19
7.5
-
-
Acidilobus saccharovorans
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.1.19
6.5
10
hydrolysis of nicotinamide of a wide range of pH values
Acidilobus saccharovorans
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
3.5.1.19
331
-
Pyrazinamide
pH 7.5, 60°C
Acidilobus saccharovorans
3.5.1.19
427
-
nicotinamide
pH 7.5, 60°C
Acidilobus saccharovorans
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
3.5.1.19
331
-
Pyrazinamide
pH 7.5, 60°C
Acidilobus saccharovorans
3.5.1.19
427
-
nicotinamide
pH 7.5, 60°C
Acidilobus saccharovorans