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Literature summary extracted from

  • Funa, N.; Ohnishi, Y.; Ebizuka, Y.; Horinouchi, S.
    Alteration of reaction and substrate specificity of a bacterial type III polyketide synthase by site-directed mutagenesis (2002), Biochem. J., 367, 781-789.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.233 expressed in Escherichia coli BL21(DE3) cells Streptomyces griseus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.233 A305I the mutant produces only a triketide pyrone from hexanoyl-CoA as starter substrate Streptomyces griseus
2.3.1.233 A305S the mutant produces a triketide and tetraketide pyrone from hexanoyl-CoA as starter substrate Streptomyces griseus
2.3.1.233 C138A the mutant loses the ability to decarboxylate malonyl-CoA Streptomyces griseus
2.3.1.233 C138Q the mutant loses the ability to decarboxylate malonyl-CoA Streptomyces griseus
2.3.1.233 C138S the mutation does not influence the decarboxylation of malonyl-CoA Streptomyces griseus
2.3.1.233 F188A inactive Streptomyces griseus
2.3.1.233 F188S inactive Streptomyces griseus
2.3.1.233 F188Y the mutant shows reduced catalytic efficiency with hexanoyl-CoA as substrate Streptomyces griseus
2.3.1.233 H270A inactive Streptomyces griseus
2.3.1.233 H270N the mutant produces triacetic acid lactone and flaviolin, an auto-oxidized product of 1,3,6,8-tetrahydroxynaphthalene, at a reduced rate Streptomyces griseus
2.3.1.233 H270Q the mutant retains high activity to catalyse polyketide formation, whereas the derailment products triacetic acid lactone and 6-acetonyl-4-hydroxy-2-pyrone (tetraketide lactone) are observed in increased amounts Streptomyces griseus
2.3.1.233 N303D inactive Streptomyces griseus
2.3.1.233 N303H inactive Streptomyces griseus
2.3.1.233 N303Q inactive Streptomyces griseus
2.3.1.233 Y224F the mutant is able to accept malonyl-CoA as a starter substrate Streptomyces griseus
2.3.1.233 Y224G the mutant shows reduced catalytic efficiency with hexanoyl-CoA as substrate Streptomyces griseus
2.3.1.233 Y224L the mutant shows increased catalytic efficiency with hexanoyl-CoA as substrate Streptomyces griseus
2.3.1.233 Y224W the mutant is able to accept malonyl-CoA as a starter substrate Streptomyces griseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.233 0.0087
-
 hexanoyl-CoA mutant enzyme A350I, at pH 7.5 and 30°C Streptomyces griseus
2.3.1.233 0.0132
-
 hexanoyl-CoA wild type enzyme, at pH 7.5 and 30°C Streptomyces griseus
2.3.1.233 0.0143
-
 hexanoyl-CoA mutant enzyme Y224L, at pH 7.5 and 30°C Streptomyces griseus
2.3.1.233 0.0157
-
 hexanoyl-CoA mutant enzyme A350S, at pH 7.5 and 30°C Streptomyces griseus
2.3.1.233 0.0508
-
 hexanoyl-CoA mutant enzyme Y224G, at pH 7.5 and 30°C Streptomyces griseus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.233 40000
-
 x * 40000, SDS-PAGE Streptomyces griseus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.233 5 malonyl-CoA Streptomyces griseus
-
 1,3,6,8-tetrahydroxynaphthalene + 5 CoA + 5 CO2 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.233 Streptomyces griseus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.233 His-binding resin column chromatography Streptomyces griseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.233 5 malonyl-CoA
-
 Streptomyces griseus 1,3,6,8-tetrahydroxynaphthalene + 5 CoA + 5 CO2 + H2O
-
 ?
2.3.1.233 hexanoyl-CoA
-
 Streptomyces griseus 4-hydroxy-6-pentyl-2-pyrone + 4-hydroxy-6-(2'-oxoheptyl)-2-pyrone + CoA + CO2 + H2O
-
 ?
2.3.1.233 phenylacetyl-CoA
-
 Streptomyces griseus 6-benzyl-4-hydroxy-2-pyrone + 4-hydroxy-6-(2'-oxo-3'-phenylpropyl)-2-pyrone + CoA + CO2 + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.233 ? x * 40000, SDS-PAGE Streptomyces griseus

Synonyms

EC Number Synonyms Comment Organism
2.3.1.233 RppA
-
 Streptomyces griseus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.233 0.6
-
 hexanoyl-CoA wild type enzyme, at pH 7.5 and 30°C Streptomyces griseus
2.3.1.233 0.65
-
 hexanoyl-CoA mutant enzyme Y224G, at pH 7.5 and 30°C Streptomyces griseus
2.3.1.233 1.45
-
 hexanoyl-CoA mutant enzyme Y224L, at pH 7.5 and 30°C Streptomyces griseus
2.3.1.233 2.07
-
 hexanoyl-CoA mutant enzyme A350I, at pH 7.5 and 30°C Streptomyces griseus
2.3.1.233 9
-
 hexanoyl-CoA mutant enzyme A350S, at pH 7.5 and 30°C Streptomyces griseus

General Information

EC Number General Information Comment Organism
2.3.1.233 metabolism the enzyme catalyses the synthesis of 1,3,6,8-tetrahydroxynaphthalene which is the key intermediate of melanin biosynthesis Streptomyces griseus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.233 12.85
-
 hexanoyl-CoA mutant enzyme Y224G, at pH 7.5 and 30°C Streptomyces griseus
2.3.1.233 45.41
-
 hexanoyl-CoA wild type enzyme, at pH 7.5 and 30°C Streptomyces griseus
2.3.1.233 101.7
-
 hexanoyl-CoA mutant enzyme Y224L, at pH 7.5 and 30°C Streptomyces griseus
2.3.1.233 238.9
-
 hexanoyl-CoA mutant enzyme A350I, at pH 7.5 and 30°C Streptomyces griseus
2.3.1.233 574.7
-
 hexanoyl-CoA mutant enzyme A350S, at pH 7.5 and 30°C Streptomyces griseus