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Literature summary extracted from

  • Garcia-Fraga, B.; da Silva, A.; Lopez-Seijas, J.; Sieiro, C.
    A novel family 19 chitinase from the marine-derived Pseudoalteromonas tunicata CCUG 44952T: heterologous expression, characterization and antifungal activity (2015), Biochem. Eng. J., 93, 84-93.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.2.1.14 pharmacology since the enzyme exhibits antifungal activity against phytopathogenic and human pathogenic fungi, it can be used as an alternative biofungicide Pseudoalteromonas tunicata

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 gene Ptchi19, DNA and amino acid sequence determination and analysis, expression in Eschereichia coli Pseudoalteromonas tunicata

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.14 Ca2+ 8% inhibition at 5 mM Pseudoalteromonas tunicata
3.2.1.14 Cd2+ 93% inhibition at 5 mM Pseudoalteromonas tunicata
3.2.1.14 Cu2+ complete inhibition at 5 mM Pseudoalteromonas tunicata
3.2.1.14 Fe2+ 88% inhibition at 5 mM Pseudoalteromonas tunicata
3.2.1.14 Hg2+ complete inhibition at 5 mM Pseudoalteromonas tunicata
3.2.1.14 K+ 10% inhibition at 5 mM Pseudoalteromonas tunicata
3.2.1.14 Mn2+ 34% inhibition at 5 mM Pseudoalteromonas tunicata
3.2.1.14 additional information poor inhibition by Mg2+ at 5 mM Pseudoalteromonas tunicata
3.2.1.14 Ni2+ 89% inhibition at 5 mM Pseudoalteromonas tunicata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 53500
-
 x * 53500, recombinant enzyme, SDS-PAGE Pseudoalteromonas tunicata

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Pseudoalteromonas tunicata
-
 gene Ptchi19
-
3.2.1.14 Pseudoalteromonas tunicata CCUG 44952T
-
 gene Ptchi19
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 recombinant enzyme 8.2fold from Escherichia coli Pseudoalteromonas tunicata

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 16.4
-
 purified recombinant enzyme, pH 7.5, 43°C, substrate crystalline chitin Pseudoalteromonas tunicata
3.2.1.14 59.9
-
 purified recombinant enzyme, pH 7.5, 43°C, substrate colloidal chitin Pseudoalteromonas tunicata
3.2.1.14 2382
-
 purified recombinant enzyme, pH 7.5, 43°C, substrate 4-nitrophenyl N-acetyl-beta-D-glucosamine Pseudoalteromonas tunicata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 4-nitrophenyl N-acetyl-beta-D-glucosamine + H2O best substrate Pseudoalteromonas tunicata 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.14 4-nitrophenyl N-acetyl-beta-D-glucosamine + H2O best substrate Pseudoalteromonas tunicata CCUG 44952T 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.14 colloidal chitin + H2O from crab shell chitin Pseudoalteromonas tunicata ?
-
 ?
3.2.1.14 colloidal chitin + H2O from crab shell chitin Pseudoalteromonas tunicata CCUG 44952T ?
-
 ?
3.2.1.14 crystalline chitin + H2O from crab shells Pseudoalteromonas tunicata ?
-
 ?
3.2.1.14 crystalline chitin + H2O from crab shells Pseudoalteromonas tunicata CCUG 44952T ?
-
 ?
3.2.1.14 additional information no activity with 4-nitrophenyl N,N',N''-beta-D-triacetylchitotriose and 4-nitrophenyl N,N'-beta-diacetylchitobiose Pseudoalteromonas tunicata ?
-
 ?
3.2.1.14 additional information no activity with 4-nitrophenyl N,N',N''-beta-D-triacetylchitotriose and 4-nitrophenyl N,N'-beta-diacetylchitobiose Pseudoalteromonas tunicata CCUG 44952T ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 53500, recombinant enzyme, SDS-PAGE Pseudoalteromonas tunicata
3.2.1.14 More multi-domain structure are the domain (D132-A155) where the active site is located, and the domain (A437-W479) that includes a C-terminal carbohydrate-binding module 5 Pseudoalteromonas tunicata

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 Ptchi19p
-
 Pseudoalteromonas tunicata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 45
-
-
 Pseudoalteromonas tunicata

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.14 20 50 recombinant enzyme, activity range Pseudoalteromonas tunicata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 7.5
-
 assay at Pseudoalteromonas tunicata

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.14 6 9.5 recombinant enzyme, activity range Pseudoalteromonas tunicata

General Information

EC Number General Information Comment Organism
3.2.1.14 evolution the enzyme belongs to the glycosyl hydrolase family GH19 Pseudoalteromonas tunicata
3.2.1.14 physiological function the enzyme exhibits antifungal activity against phytopathogenic and human pathogenic fungi Pseudoalteromonas tunicata