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Literature summary extracted from

  • Yang, G.; Yang, G.; Aprile, L.; Turturo, V.; Pucciarelli, S.; Pucciarelli, S.; Miceli, C.
    Characterization and comparative analysis of psychrophilic and mesophilic alpha-amylases from Euplotes species: a contribution to the understanding of enzyme thermal adaptation (2013), Biochem. Biophys. Res. Commun., 438, 715-720.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.1 expression in Escherichia coli Euplotes focardii
3.2.1.1 expression in Escherichia coli Moneuplotes crassus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.1 additional information
-
 starch KM value is 1.31 g/l at 5°C, 1.36 g/l at 25°C, and 1.42 g/l at 35°C respectively, and pH 9.0 Moneuplotes crassus
3.2.1.1 additional information
-
 starch KM value is 3 g/l at 5°C, 3.3g/l at 25°C, and 4.4 g/l at 35°C respectively, and pH 9.0 Euplotes focardii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.1 52000
-
 x * 52000, SDS-PAGE Euplotes focardii
3.2.1.1 53000
-
 x * 53000, SDS-PAGE Moneuplotes crassus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Euplotes focardii T1WE96
-
-
3.2.1.1 Moneuplotes crassus T1WDH2
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 starch + H2O
-
 Euplotes focardii ?
-
 ?
3.2.1.1 starch + H2O
-
 Moneuplotes crassus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.1 ? x * 53000, SDS-PAGE Moneuplotes crassus
3.2.1.1 ? x * 52000, SDS-PAGE Euplotes focardii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.1 20 25
-
 Euplotes focardii
3.2.1.1 35
-
-
 Moneuplotes crassus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.1
-
-
 50% of maximum activity Euplotes focardii
3.2.1.1 45
-
 10% of maximum activity Euplotes focardii
3.2.1.1 60
-
 20% of maximum activity Moneuplotes crassus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.1 40
-
 half-life 6.5 min Moneuplotes crassus
3.2.1.1 40
-
 half-life 4.1 min Euplotes focardii
3.2.1.1 50
-
 half-life 1.8 min Euplotes focardii
3.2.1.1 50
-
 half-life 4.2 min Moneuplotes crassus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.1 377
-
 starch 5°C, pH 9.0 Moneuplotes crassus
3.2.1.1 619
-
 starch 25°C, pH 9.0 Moneuplotes crassus
3.2.1.1 718
-
 starch 5°C, pH 9.0 Euplotes focardii
3.2.1.1 880
-
 starch 35°C, pH 9.0 Moneuplotes crassus
3.2.1.1 982
-
 starch 35°C, pH 9.0 Euplotes focardii
3.2.1.1 1466
-
 starch 25°C, pH 9.0 Euplotes focardii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.1 9
-
-
 Euplotes focardii
3.2.1.1 9
-
-
 Moneuplotes crassus