EC Number | Application | Comment | Organism |
---|---|---|---|
4.2.2.2 | industry | pectate lyase (Bsp165PelA) from Bacillus sp. N16-5 has a great potential in industrial applications because it shows high specific activity under extremely alkaline conditions | Bacillus sp. (in: Bacteria) |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
4.2.2.2 | purified enzyme, alone or complexed with trigalacturonate, sitting drop method, 0.002 ml of 12.5 mg/ml protein in 25 mM Tris-HCl, 150 mM NaCl, pH 8.0, are mixed with 0.002 ml of 0.18 M Li2SO4, 0.085 M Tris-HCl, pH 8.5, 24% PEG 4000, and 15% v/v glycerol anhydrous, and equilibrated against 0.5 ml of well solution, three days, X-ray diffraction structure determination and analysis at 1.5-1.9 A resolution | Bacillus sp. (in: Bacteria) |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.2.2.2 | Ca2+ | dependent on, binding structure, overview | Bacillus sp. (in: Bacteria) |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.2.2 | Bacillus sp. (in: Bacteria) | - |
- |
- |
4.2.2.2 | Bacillus sp. (in: Bacteria) N16-5 | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.2.2 | trigalacturonate | enzyme and substrate-binding structures, overview | Bacillus sp. (in: Bacteria) | ? | - |
? | |
4.2.2.2 | trigalacturonate | enzyme and substrate-binding structures, overview | Bacillus sp. (in: Bacteria) N16-5 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.2.2 | Bsp165PelA | - |
Bacillus sp. (in: Bacteria) |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.2.2 | 50 | - |
- |
Bacillus sp. (in: Bacteria) |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.2.2 | 11.5 | - |
- |
Bacillus sp. (in: Bacteria) |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.2.2.2 | evolution | parallel beta-helix, active site residues, and substrate binding cleft are similar to those in the other pectate lyases from polysaccharide lyase family 1, PL1 | Bacillus sp. (in: Bacteria) |