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Literature summary extracted from

  • Kodera, T.; Smirnov, S.; Samsonova, N.; Kozlov, Y.; Koyama, R.; Hibi, M.; Ogawa, J.; Yokozeki, K.; Shimizu, S.
    A novel l-isoleucine hydroxylating enzyme, l-isoleucine dioxygenase from Bacillus thuringiensis, produces (2S,3R,4S)-4-hydroxyisoleucine (2009), Biochem. Biophys. Res. Commun., 390, 506-510.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.11.45 ascorbic acid ascorbic acid is not necessarily required for the reaction, however, in the presence of ascorbic acid, the reaction rate is greatly stimulated Bacillus thuringiensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.45 Cu2+ complete inhibition at 1 mM Bacillus thuringiensis
1.14.11.45 EDTA complete inhibition at 1 mM Bacillus thuringiensis
1.14.11.45 Zn2+ complete inhibition at 1 mM Bacillus thuringiensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.45 Fe2+ required for activity Bacillus thuringiensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.11.45 31000
-
 x * 31000, SDS-PAGE Bacillus thuringiensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.45 L-isoleucine + 2-oxoglutarate + O2 Bacillus thuringiensis
-
 (2S,3R,4S)-4-hydroxy-isoleucine + succinate + CO2
-
 ?
1.14.11.45 L-isoleucine + 2-oxoglutarate + O2 Bacillus thuringiensis 2e2 AKU 0251
-
 (2S,3R,4S)-4-hydroxy-isoleucine + succinate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.45 Bacillus thuringiensis
-
-
-
1.14.11.45 Bacillus thuringiensis 2e2 AKU 0251
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.45 HiPrep DEAE column chromatography, Mono S column chromatography, Resource PHE column chromatography, and Superdex 75 gel filtration Bacillus thuringiensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.45 L-isoleucine + 2-oxoglutarate + O2
-
 Bacillus thuringiensis (2S,3R,4S)-4-hydroxy-isoleucine + succinate + CO2
-
 ?
1.14.11.45 L-isoleucine + 2-oxoglutarate + O2
-
 Bacillus thuringiensis 2e2 AKU 0251 (2S,3R,4S)-4-hydroxy-isoleucine + succinate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.11.45 ? x * 31000, SDS-PAGE Bacillus thuringiensis

Synonyms

EC Number Synonyms Comment Organism
1.14.11.45 IDO
-
 Bacillus thuringiensis
1.14.11.45 L-isoleucine dioxygenase
-
 Bacillus thuringiensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.11.45 30
-
-
 Bacillus thuringiensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.11.45 6
-
-
 Bacillus thuringiensis