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Literature summary extracted from

  • Sun, Y.; Lv, X.; Li, Z.; Wang, J.; Jia, B.; Liu, J.
    Recombinant cyclodextrinase from Thermococcus kodakarensis KOD1: expression, purification, and enzymatic characterization (2015), Archaea, 2015, 397924.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.54 expression in Escherichia coli Thermococcus kodakarensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.54 additional information
-
 beta-cyclodextrin KM-value is 2.94 mg/ml at pH 7.5 and 65°C Thermococcus kodakarensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.54 76400
-
 x * 76400, calculated from sequence Thermococcus kodakarensis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.54 Thermococcus kodakarensis Q5JJ59
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.54
-
 Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.54 beta-cyclodextrin + H2O beta-cyclodextrin is the most active substrate Thermococcus kodakarensis D-glucose + ?
-
 ?
3.2.1.54 gamma-cyclodextrin + H2O hydrolyzing activity is about 20% compared to the activity with beta-cyclodextrin Thermococcus kodakarensis D-glucose + ?
-
 ?
3.2.1.54 pullulan + H2O hydrolyzing activity is about 20% compared to the activity with beta-cyclodextrin Thermococcus kodakarensis D-glucose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.54 ? x * 76400, calculated from sequence Thermococcus kodakarensis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.54 TK1770 locus name Thermococcus kodakarensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.54 65
-
-
 Thermococcus kodakarensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.54 50 90 50°C: about 55% of maximal activity, 90°C: about 80% of maximal activity Thermococcus kodakarensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.54 7.5
-
-
 Thermococcus kodakarensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.54 3 10 pH 3.0: about 85% of maximal activity, pH 10.0: about 85% of maximal activity Thermococcus kodakarensis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.54 Thermococcus kodakarensis calculated from sequence
-
 5.5

General Information

EC Number General Information Comment Organism
3.2.1.54 physiological function the characteristic of the enzyme hydrolyzing beta-cyclodextrin at a relatively low temperature and nonneutral pH should play an important role in the survival of Thermococcus kodakarensis KOD1 under low temperature conditions (65°C) Thermococcus kodakarensis