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Literature summary extracted from

  • Mittelstaedt, G.; Negron, L.; Schofield, L.R.; Marsh, K.; Parker, E.J.
    Biochemical and structural characterisation of dehydroquinate synthase from the New Zealand kiwifruit Actinidia chinensis (2013), Arch. Biochem. Biophys., 537, 185-191.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.4 expression in Escherichia coli Actinidia chinensis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.3.4 molecular replacement based on the structure of Aspergillus nidulans enzyme, PDB code 1NR5, revealing a homodimeric protein. Each monomer has a NAD+ binding site nestled between the distinct N- and C-terminal domains Actinidia chinensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.4 1.3
-
 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate pH 7.5, 25°C Actinidia chinensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.4 Co2+
-
 Actinidia chinensis
4.2.3.4 Fe2+ 110% of the activity in presence of Co2+ Actinidia chinensis
4.2.3.4 additional information a divalent metal ion is required Actinidia chinensis
4.2.3.4 Zn2+ 25% of the activity in presence of Co2+ Actinidia chinensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.4 41097
-
 2 * 41097, mass spectrometry, 2 * 41098, calculated Actinidia chinensis
4.2.3.4 41098
-
 2 * 41097, mass spectrometry, 2 * 41098, calculated Actinidia chinensis
4.2.3.4 79200
-
 light scattering Actinidia chinensis
4.2.3.4 81000
-
 gel filtration Actinidia chinensis

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.4 Actinidia chinensis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.4 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate
-
 Actinidia chinensis 3-dehydroquinate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.3.4 dimer 2 * 41097, mass spectrometry, 2 * 41098, calculated Actinidia chinensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.3.4 41
-
 melting temperature, in presence of either Zn2+ or Co2+ Actinidia chinensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.3.4 20.1
-
 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate pH 7.5, 25°C Actinidia chinensis

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.3.4 additional information no cofactor: NADP+ Actinidia chinensis
4.2.3.4 NAD+ required Actinidia chinensis