EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.3.11 | expression in Escherichia coli | Thymus vulgaris |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.3.11 | I346N | mutation to the corresponding residue in isoform Tps6. Mutation alters the stereospecificity to that of TPS6. (1S,2S,4R)-(E)-sabinene hydrate is still the main product of this mutant, but I346N also produces the (4S)-enantiomers of (E)-sabinene hydrate and (Z)-sabinene hydrate | Thymus vulgaris |
4.2.3.11 | N350I | mutation to the corresponding residue in isoform Tps7. Mutation alters the stereospecificity to that of TPS7, mutant produces only the (1S,2S,4R)-enantiomer of (E)-sabinene hydrate | Thymus vulgaris |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.3.11 | Thymus vulgaris | L0HAM7 | isoform Tps6 | - |
4.2.3.11 | Thymus vulgaris | L0HB77 | isoform Tps7 | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.3.11 | (3R)-linalyl diphosphate + H2O | - |
Thymus vulgaris | (1S,2S,4R)-(E)-sabinene hydrate + diphosphate | exclusive product of both isoforms Tps6 and Tps7 | ? | |
4.2.3.11 | (3S)-linalyl diphosphate + H2O | - |
Thymus vulgaris | (1R,2R,4S)-(E)-sabinene hydrate + diphosphate | - |
? | |
4.2.3.11 | (3S)-linalyl diphosphate + H2O | - |
Thymus vulgaris | (1S,2R,4S)-(Z)-sabinene hydrate + diphosphate | no formation of the stereoisomer (1R,2S,4R)-(Z)-sabinene hydrate | ? | |
4.2.3.11 | geranyl diphosphate + H2O | - |
Thymus vulgaris | (1S,2R,4S)-(Z)-sabinene hydrate + diphosphate | the stereospecificity of the reaction is determined by the formation of the first intermediate, linalyl diphosphate. Products of isoform Tps6 are 60.4% (Z)-sabinene hydrate, 20.9% (E)-sabinene hydrate, 3.2% alpha-pinene, 2.6% myrcene plus traces of 12 more monoterpenes | ? | |
4.2.3.11 | geranyl diphosphate + H2O | - |
Thymus vulgaris | (1S,2S,4R)-(E)-sabinene hydrate + diphosphate | the stereospecificity of the reaction is determined by the formation of the first intermediate, linalyl diphosphate. Products of isoform Tps7 are 77.8% (1S,2S,4R)-(E)-sabinene hydrate, 6.3% limonene, 1.43% (Z)-sabinene hydrate plus traces of other monoterpenes | ? | |
4.2.3.11 | geranyl diphosphate + H2O | - |
Thymus vulgaris | (E)-sabinene hydrate + diphosphate | the stereospecificity of the reaction is determined by the formation of the first intermediate, linalyl diphosphate. Products of isoform Tps6 are 60.4% (Z)-sabinene hydrate, 20.9% (E)-sabinene hydrate, 3.2% alpha-pinene, 2.6% myrcene plus traces of 12 more monoterpenes | ? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.3.11 | TPS6 | - |
Thymus vulgaris |
4.2.3.11 | Tps7 | - |
Thymus vulgaris |