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Literature summary extracted from

  • Lahiri, S.; Basu, A.; Sengupta, S.; Banerjee, S.; Dutta, T.; Soren, D.; Chattopadhyay, K.; Ghosh, A.K.
    Purification and characterization of a trehalase-invertase enzyme with dual activity from Candida utilis (2012), Arch. Biochem. Biophys., 522, 90-99.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.28 Citric acid 36.16% residual activity at 5 mM Cyberlindnera jadinii
3.2.1.28 EDTA 18.18% residual activity at 5 mM Cyberlindnera jadinii
3.2.1.28 KH2PO4 10.32% residual activity at 200 mM Cyberlindnera jadinii
3.2.1.28 MgSO4 57.02% residual activity at 2 mM Cyberlindnera jadinii
3.2.1.28 ZnSO4 2.06% residual activity at 10 mM Cyberlindnera jadinii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.28 57000
-
 3 * 57000, SDS-PAGE Cyberlindnera jadinii
3.2.1.28 175000
-
 gel filtration Cyberlindnera jadinii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.28 alpha,alpha-trehalose + H2O Cyberlindnera jadinii
-
 beta-D-glucose + alpha-D-glucose
-
 ?
3.2.1.28 additional information Cyberlindnera jadinii trehalose-invertase is specific for both sucrose and trehalose. The ratio between trehalase and invertase activity is 1:3.5 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.28 Cyberlindnera jadinii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.28 ammonium sulfate precipitation, Biosuite DEAE column chromatography, and gel filtration Cyberlindnera jadinii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.28 0.01
-
 supernatant, at pH 7.0 and 30°C Cyberlindnera jadinii
3.2.1.28 0.405
-
 after 40fold purification, at pH 7.0 and 30°C Cyberlindnera jadinii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.28 alpha,alpha-trehalose + H2O
-
 Cyberlindnera jadinii beta-D-glucose + alpha-D-glucose
-
 ?
3.2.1.28 additional information trehalose-invertase is specific for both sucrose and trehalose. The ratio between trehalase and invertase activity is 1:3.5 Cyberlindnera jadinii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.28 homotrimer 3 * 57000, SDS-PAGE Cyberlindnera jadinii

Synonyms

EC Number Synonyms Comment Organism
3.2.1.28 NT-INV
-
 Cyberlindnera jadinii
3.2.1.28 trehalase-invertase
-
 Cyberlindnera jadinii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.28 30
-
-
 Cyberlindnera jadinii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.28 70
-
 no activity after 15 min at 70°C Cyberlindnera jadinii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.28 7
-
-
 Cyberlindnera jadinii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.28 4.5 9 no activity at pH values less than 4.5 or more than 9.0 Cyberlindnera jadinii