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Literature summary extracted from

  • Sirotkina, M.; Efremenko, E.N.
    Rhodococcus lactonase with organophosphate hydrolase (OPH) activity and His6-tagged OPH with lactonase activity: evolutionary proximity of the enzymes and new possibilities in their application (2014), Appl. Microbiol. Biotechnol., 98, 2647-2656.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.81 0.101
-
 N-(3-oxododecyl)-L-homoserine lactone pH 8.2, 25°C Brevundimonas diminuta
3.1.1.81 0.184
-
 N-(3-oxodecyl)-L-homoserine lactone pH 8.2, 25°C Brevundimonas diminuta
3.1.1.81 0.306
-
 L-homoserine lactone pH 8.2, 25°C Brevundimonas diminuta
3.1.1.81 0.412
-
 N-butyryl-DL-homoserine lactone pH 8.2, 25°C Brevundimonas diminuta
3.1.1.81 0.526
-
 N-hexyl-L-homoserine lactone pH 8.2, 25°C Brevundimonas diminuta
3.1.1.81 0.608
-
 N-(3-oxooctyl)-L-homoserine lactone pH 8.2, 25°C Brevundimonas diminuta

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.81 Brevundimonas diminuta
-
-
-
3.1.1.81 Rhodococcus erythropolis
-
-
-
3.1.1.81 Rhodococcus erythropolis AC-1514D
-
-
-
3.1.1.81 Rhodococcus ruber
-
-
-
3.1.1.81 Rhodococcus ruber AC-1513D
-
-
-
3.1.8.1 Rhodococcus erythropolis
-
-
-
3.1.8.1 Rhodococcus erythropolis AC-1514D
-
-
-
3.1.8.1 Rhodococcus ruber
-
-
-
3.1.8.1 Rhodococcus ruber AC-1513D
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.81 L-homoserine lactone + H2O
-
 Rhodococcus erythropolis L-homoserine
-
 ?
3.1.1.81 L-homoserine lactone + H2O
-
 Rhodococcus ruber L-homoserine
-
 ?
3.1.1.81 L-homoserine lactone + H2O
-
 Brevundimonas diminuta L-homoserine
-
 ?
3.1.1.81 additional information enzyme additionally displays organophosphate hydrolase activity, EC 3.1.8.1 Rhodococcus erythropolis ?
-
 ?
3.1.1.81 additional information enzyme additionally displays organophosphate hydrolase activity, EC 3.1.8.1 Rhodococcus ruber ?
-
 ?
3.1.1.81 additional information enzyme additionally displays organophosphate hydrolase activity, EC 3.1.8.1 Brevundimonas diminuta ?
-
 ?
3.1.1.81 N-(3-oxodecyl)-L-homoserine lactone + H2O
-
 Rhodococcus erythropolis N-(3-oxodecyl)-L-homoserine
-
 ?
3.1.1.81 N-(3-oxodecyl)-L-homoserine lactone + H2O
-
 Rhodococcus ruber N-(3-oxodecyl)-L-homoserine
-
 ?
3.1.1.81 N-(3-oxodecyl)-L-homoserine lactone + H2O
-
 Brevundimonas diminuta N-(3-oxodecyl)-L-homoserine
-
 ?
3.1.1.81 N-(3-oxododecyl)-L-homoserine lactone + H2O
-
 Rhodococcus erythropolis N-(3-oxododecyl)-L-homoserine
-
 ?
3.1.1.81 N-(3-oxododecyl)-L-homoserine lactone + H2O
-
 Rhodococcus ruber N-(3-oxododecyl)-L-homoserine
-
 ?
3.1.1.81 N-(3-oxododecyl)-L-homoserine lactone + H2O
-
 Brevundimonas diminuta N-(3-oxododecyl)-L-homoserine
-
 ?
3.1.1.81 N-(3-oxooctyl)-L-homoserine lactone + H2O
-
 Rhodococcus erythropolis N-(3-oxooctyl)-L-homoserine
-
 ?
3.1.1.81 N-(3-oxooctyl)-L-homoserine lactone + H2O
-
 Rhodococcus ruber N-(3-oxooctyl)-L-homoserine
-
 ?
3.1.1.81 N-(3-oxooctyl)-L-homoserine lactone + H2O
-
 Brevundimonas diminuta N-(3-oxooctyl)-L-homoserine
-
 ?
3.1.1.81 N-butyryl-DL-homoserine lactone + H2O
-
 Rhodococcus erythropolis N-butyryl-DL-homoserine
-
 ?
3.1.1.81 N-butyryl-DL-homoserine lactone + H2O
-
 Rhodococcus ruber N-butyryl-DL-homoserine
-
 ?
3.1.1.81 N-butyryl-DL-homoserine lactone + H2O
-
 Brevundimonas diminuta N-butyryl-DL-homoserine
-
 ?
3.1.1.81 N-hexyl-L-homoserine lactone + H2O
-
 Rhodococcus erythropolis N-hexyl-L-homoserine
-
 ?
3.1.1.81 N-hexyl-L-homoserine lactone + H2O
-
 Rhodococcus ruber N-hexyl-L-homoserine
-
 ?
3.1.1.81 N-hexyl-L-homoserine lactone + H2O
-
 Brevundimonas diminuta N-hexyl-L-homoserine
-
 ?
3.1.8.1 demeton-S + H2O 0.2 mM, 26% degradation over 24 h by whole cells Rhodococcus erythropolis 2-ethylsulfanyl-ethanethiol + diethyl phosphate
-
 ?
3.1.8.1 demeton-S + H2O 0.2 mM, 27% degradation over 24 h by whole cells Rhodococcus ruber 2-ethylsulfanyl-ethanethiol + diethyl phosphate
-
 ?
3.1.8.1 demeton-S + H2O 0.2 mM, 27% degradation over 24 h by whole cells Rhodococcus ruber AC-1513D 2-ethylsulfanyl-ethanethiol + diethyl phosphate
-
 ?
3.1.8.1 demeton-S + H2O 0.2 mM, 26% degradation over 24 h by whole cells Rhodococcus erythropolis AC-1514D 2-ethylsulfanyl-ethanethiol + diethyl phosphate
-
 ?
3.1.8.1 diazinon + H2O 0.2 mM, 24% degradation over 24 h by whole cells Rhodococcus erythropolis ?
-
 ?
3.1.8.1 diazinon + H2O 0.2 mM, 60% degradation over 24 h by whole cells Rhodococcus ruber ?
-
 ?
3.1.8.1 dimethoate + H2O 0.2 mM, 14% degradation over 24 h by whole cells Rhodococcus erythropolis ?
-
 ?
3.1.8.1 dimethoate + H2O 0.2 mM, 16% degradation over 24 h by whole cells Rhodococcus ruber ?
-
 ?
3.1.8.1 dimethoate + H2O 0.2 mM, 16% degradation over 24 h by whole cells Rhodococcus ruber AC-1513D ?
-
 ?
3.1.8.1 dimethoate + H2O 0.2 mM, 14% degradation over 24 h by whole cells Rhodococcus erythropolis AC-1514D ?
-
 ?
3.1.8.1 malathion + H2O 0.2 mM, 89% degradation over 24 h by whole cells Rhodococcus erythropolis O,O-diethyl phosphorothioate + diethyl 2-mercaptosuccinate
-
 ?
3.1.8.1 malathion + H2O 0.2 mM, 95% degradation over 24 h by whole cells Rhodococcus ruber O,O-diethyl phosphorothioate + diethyl 2-mercaptosuccinate
-
 ?
3.1.8.1 malathion + H2O 0.2 mM, 95% degradation over 24 h by whole cells Rhodococcus ruber AC-1513D O,O-diethyl phosphorothioate + diethyl 2-mercaptosuccinate
-
 ?
3.1.8.1 malathion + H2O 0.2 mM, 89% degradation over 24 h by whole cells Rhodococcus erythropolis AC-1514D O,O-diethyl phosphorothioate + diethyl 2-mercaptosuccinate
-
 ?
3.1.8.1 additional information enzyme additionally displays L-homoserine lactonase activity, EC 3.1.1.81 Rhodococcus erythropolis ?
-
 ?
3.1.8.1 additional information enzyme additionally displays L-homoserine lactonase activity, EC 3.1.1.81 Rhodococcus ruber ?
-
 ?
3.1.8.1 additional information enzyme additionally displays L-homoserine lactonase activity, EC 3.1.1.81 Rhodococcus ruber AC-1513D ?
-
 ?
3.1.8.1 additional information enzyme additionally displays L-homoserine lactonase activity, EC 3.1.1.81 Rhodococcus erythropolis AC-1514D ?
-
 ?
3.1.8.1 paraoxon + H2O 0.2 mM, 12% degradation over 24 h by whole cells Rhodococcus erythropolis 4-nitrophenol + diethyl phosphate
-
 ?
3.1.8.1 paraoxon + H2O 0.2 mM, 35% degradation over 24 h by whole cells Rhodococcus ruber 4-nitrophenol + diethyl phosphate
-
 ?
3.1.8.1 paraoxon + H2O 0.2 mM, 35% degradation over 24 h by whole cells Rhodococcus ruber AC-1513D 4-nitrophenol + diethyl phosphate
-
 ?
3.1.8.1 paraoxon + H2O 0.2 mM, 12% degradation over 24 h by whole cells Rhodococcus erythropolis AC-1514D 4-nitrophenol + diethyl phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.81 OPH
-
 Brevundimonas diminuta

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.81 1.83
-
 N-(3-oxododecyl)-L-homoserine lactone pH 8.2, 25°C Brevundimonas diminuta
3.1.1.81 2.62
-
 N-butyryl-DL-homoserine lactone pH 8.2, 25°C Brevundimonas diminuta
3.1.1.81 2.67
-
 N-(3-oxodecyl)-L-homoserine lactone pH 8.2, 25°C Brevundimonas diminuta
3.1.1.81 3.17
-
 N-hexyl-L-homoserine lactone pH 8.2, 25°C Brevundimonas diminuta
3.1.1.81 4.66
-
 N-(3-oxooctyl)-L-homoserine lactone pH 8.2, 25°C Brevundimonas diminuta
3.1.1.81 4.69
-
 L-homoserine lactone pH 8.2, 25°C Brevundimonas diminuta

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.81 6.35
-
 N-butyryl-DL-homoserine lactone pH 8.2, 25°C Brevundimonas diminuta
3.1.1.81 6.35
-
 N-hexyl-L-homoserine lactone pH 8.2, 25°C Brevundimonas diminuta
3.1.1.81 7.66
-
 N-(3-oxooctyl)-L-homoserine lactone pH 8.2, 25°C Brevundimonas diminuta
3.1.1.81 14.5
-
 N-(3-oxodecyl)-L-homoserine lactone pH 8.2, 25°C Brevundimonas diminuta
3.1.1.81 15.3
-
 L-homoserine lactone pH 8.2, 25°C Brevundimonas diminuta
3.1.1.81 18.1
-
 N-(3-oxododecyl)-L-homoserine lactone pH 8.2, 25°C Brevundimonas diminuta