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Literature summary extracted from

  • Cheng, Y.; Ko, T.; Huang, J.; Wu, T.; Lin, C.; Luo, W.; Li, Q.; Ma, Y.; Huang, C.; Wang, A.; Liu, J.; Guo, R.
    Enhanced activity of Thermotoga maritima cellulase 12A by mutating a unique surface loop (2012), Appl. Microbiol. Biotechnol., 95, 661-669.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.4 mutants Y16G, Y61GG and Y61del in complex with substrate beta-glucan. Mutations at the Tyr61 site do not affect the overall protein structure, but local perturbations might diminish the substrate-binding strength Thermotoga maritima

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.4 R60A about 45% of wild-type activity Thermotoga maritima
3.2.1.4 R60K about 70% of wild-type activity Thermotoga maritima
3.2.1.4 Y61A about 120% of wild-type activity Thermotoga maritima
3.2.1.4 Y61del about 10% of wild-type activity Thermotoga maritima
3.2.1.4 Y61F about 140% of wild-type activity Thermotoga maritima
3.2.1.4 Y61G about 170% of wild-type activity. Mutant also shows a wider range of working temperatures than does the wild type, along with retention of the hyperthermostability. The kcat and Km values of Y61G are both higher than those of the wild type. The higher endoglucanase activity is probably due to facile dissociation of the cleaved sugar moiety at the reducing end Thermotoga maritima
3.2.1.4 Y61GG insertion mutant, about 40% of wild-type activity Thermotoga maritima
3.2.1.4 Y61R about 70% of wild-type activity Thermotoga maritima
3.2.1.4 Y61W about 80% of wild-type activity Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.4 additional information
-
 beta-Glucan Km value for wild-type is 2.08 mg/ml, for mutant Y61G 3.75 mg/ml, pH 5.0, 85°C Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Thermotoga maritima Q60032
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 beta-glucan + H2O
-
 Thermotoga maritima ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 CelA
-
 Thermotoga maritima
3.2.1.4 cellulase 12A
-
 Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 85
-
 wild-type Thermotoga maritima
3.2.1.4 100
-
 mutant Y61G Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 85
-
 half-life 8 h, both wild-type and mutant Y61G Thermotoga maritima
3.2.1.4 95
-
 half-life 40 min, both wild-type and mutant Y61G Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.4 791
-
 beta-Glucan wild-type, pH 5.0, 85°C Thermotoga maritima
3.2.1.4 1155
-
 beta-Glucan mutant Y61G, pH 5.0, 85°C Thermotoga maritima

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.4 additional information
-
 beta-Glucan Kcat/Km value for wild-type is 402 mg per s and ml, for mutant Y61G 313 mg per s and ml, pH 5.0, 85°C Thermotoga maritima