BRENDA - Enzyme Database

Characterization of a thermostable methylaspartate ammonia lyase from Carboxydothermus hydrogenoformans

Raj, H.; Puthan Veetil, V.; Szymanski, W.; Dekker, F.J.; Quax, W.J.; Feringa, B.L.; Janssen, D.B.; Poelarends, G.J.; Appl. Microbiol. Biotechnol. 94, 385-397 (2012)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
4.3.1.2
overexpressed in Escherichia coli
Carboxydothermus hydrogenoformans
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.3.1.2
4.6
-
(2S,3S)-3-ethylaspartic acid
pH 9, 30°C
Carboxydothermus hydrogenoformans
4.3.1.2
16
-
(2S,3S)-3-ethylaspartic acid
pH 9, 70°C
Carboxydothermus hydrogenoformans
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.3.1.2
K+
required for maximum activity
Carboxydothermus hydrogenoformans
4.3.1.2
Mg2+
required for maximum activity
Carboxydothermus hydrogenoformans
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.1.2
49000
-
SDS-PAGE
Carboxydothermus hydrogenoformans
Organism
EC Number
Organism
UniProt
Commentary
Textmining
4.3.1.2
Carboxydothermus hydrogenoformans
Q3AEU2
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
4.3.1.2
using metal affinity chromatography
Carboxydothermus hydrogenoformans
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
4.3.1.2
(2S,3S)-3-ethylaspartic acid
-
729057
Carboxydothermus hydrogenoformans
?
-
-
-
?
4.3.1.2
ethylfumarate + NH3
-
729057
Carboxydothermus hydrogenoformans
?
-
-
-
?
4.3.1.2
fumarate + NH3
-
729057
Carboxydothermus hydrogenoformans
?
-
-
-
?
4.3.1.2
mesaconate + ethylamine
-
729057
Carboxydothermus hydrogenoformans
?
-
-
-
?
4.3.1.2
mesaconate + hydrazine
-
729057
Carboxydothermus hydrogenoformans
?
-
-
-
?
4.3.1.2
mesaconate + hydroxylamine
-
729057
Carboxydothermus hydrogenoformans
?
-
-
-
?
4.3.1.2
mesaconate + methoxylamine
-
729057
Carboxydothermus hydrogenoformans
?
-
-
-
?
4.3.1.2
mesaconate + methylamine
-
729057
Carboxydothermus hydrogenoformans
?
-
-
-
?
4.3.1.2
mesaconate + NH3
-
729057
Carboxydothermus hydrogenoformans
?
-
-
-
?
4.3.1.2
methylfumarate + NH3
-
729057
Carboxydothermus hydrogenoformans
?
-
-
-
?
4.3.1.2
propylfumarate + NH3
-
729057
Carboxydothermus hydrogenoformans
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
4.3.1.2
dimer
2 * 49000
Carboxydothermus hydrogenoformans
Synonyms
EC Number
Synonyms
Commentary
Organism
4.3.1.2
Ch-MAL
-
Carboxydothermus hydrogenoformans
4.3.1.2
methylaspartate ammonia lyase
-
Carboxydothermus hydrogenoformans
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.3.1.2
70
-
-
Carboxydothermus hydrogenoformans
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.3.1.2
50
-
stable for more than 4 hours
Carboxydothermus hydrogenoformans
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.3.1.2
16
-
(2S,3S)-3-ethylaspartic acid
pH 9, 30°C
Carboxydothermus hydrogenoformans
4.3.1.2
78
-
(2S,3S)-3-ethylaspartic acid
pH 9, 70°C
Carboxydothermus hydrogenoformans
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.3.1.2
9
-
-
Carboxydothermus hydrogenoformans
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.3.1.2
overexpressed in Escherichia coli
Carboxydothermus hydrogenoformans
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.3.1.2
4.6
-
(2S,3S)-3-ethylaspartic acid
pH 9, 30°C
Carboxydothermus hydrogenoformans
4.3.1.2
16
-
(2S,3S)-3-ethylaspartic acid
pH 9, 70°C
Carboxydothermus hydrogenoformans
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.3.1.2
K+
required for maximum activity
Carboxydothermus hydrogenoformans
4.3.1.2
Mg2+
required for maximum activity
Carboxydothermus hydrogenoformans
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.1.2
49000
-
SDS-PAGE
Carboxydothermus hydrogenoformans
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.3.1.2
using metal affinity chromatography
Carboxydothermus hydrogenoformans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
4.3.1.2
(2S,3S)-3-ethylaspartic acid
-
729057
Carboxydothermus hydrogenoformans
?
-
-
-
?
4.3.1.2
ethylfumarate + NH3
-
729057
Carboxydothermus hydrogenoformans
?
-
-
-
?
4.3.1.2
fumarate + NH3
-
729057
Carboxydothermus hydrogenoformans
?
-
-
-
?
4.3.1.2
mesaconate + ethylamine
-
729057
Carboxydothermus hydrogenoformans
?
-
-
-
?
4.3.1.2
mesaconate + hydrazine
-
729057
Carboxydothermus hydrogenoformans
?
-
-
-
?
4.3.1.2
mesaconate + hydroxylamine
-
729057
Carboxydothermus hydrogenoformans
?
-
-
-
?
4.3.1.2
mesaconate + methoxylamine
-
729057
Carboxydothermus hydrogenoformans
?
-
-
-
?
4.3.1.2
mesaconate + methylamine
-
729057
Carboxydothermus hydrogenoformans
?
-
-
-
?
4.3.1.2
mesaconate + NH3
-
729057
Carboxydothermus hydrogenoformans
?
-
-
-
?
4.3.1.2
methylfumarate + NH3
-
729057
Carboxydothermus hydrogenoformans
?
-
-
-
?
4.3.1.2
propylfumarate + NH3
-
729057
Carboxydothermus hydrogenoformans
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.3.1.2
dimer
2 * 49000
Carboxydothermus hydrogenoformans
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.3.1.2
70
-
-
Carboxydothermus hydrogenoformans
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.3.1.2
50
-
stable for more than 4 hours
Carboxydothermus hydrogenoformans
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.3.1.2
16
-
(2S,3S)-3-ethylaspartic acid
pH 9, 30°C
Carboxydothermus hydrogenoformans
4.3.1.2
78
-
(2S,3S)-3-ethylaspartic acid
pH 9, 70°C
Carboxydothermus hydrogenoformans
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.3.1.2
9
-
-
Carboxydothermus hydrogenoformans
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4.3.1.2
4.9
-
(2S,3S)-3-ethylaspartic acid
pH 9, 30°C
Carboxydothermus hydrogenoformans
4.3.1.2
35
-
(2S,3S)-3-ethylaspartic acid
pH 9, 70°C
Carboxydothermus hydrogenoformans
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4.3.1.2
4.9
-
(2S,3S)-3-ethylaspartic acid
pH 9, 30°C
Carboxydothermus hydrogenoformans
4.3.1.2
35
-
(2S,3S)-3-ethylaspartic acid
pH 9, 70°C
Carboxydothermus hydrogenoformans