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Literature summary extracted from

  • Raj, H.; Puthan Veetil, V.; Szymanski, W.; Dekker, F.J.; Quax, W.J.; Feringa, B.L.; Janssen, D.B.; Poelarends, G.J.
    Characterization of a thermostable methylaspartate ammonia lyase from Carboxydothermus hydrogenoformans (2012), Appl. Microbiol. Biotechnol., 94, 385-397.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.1.2 overexpressed in Escherichia coli Carboxydothermus hydrogenoformans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.2 4.6
-
 (2S,3S)-3-ethylaspartic acid pH 9, 30°C Carboxydothermus hydrogenoformans
4.3.1.2 16
-
 (2S,3S)-3-ethylaspartic acid pH 9, 70°C Carboxydothermus hydrogenoformans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.3.1.2 K+ required for maximum activity Carboxydothermus hydrogenoformans
4.3.1.2 Mg2+ required for maximum activity Carboxydothermus hydrogenoformans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.2 49000
-
 SDS-PAGE Carboxydothermus hydrogenoformans

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.2 Carboxydothermus hydrogenoformans Q3AEU2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.2 using metal affinity chromatography Carboxydothermus hydrogenoformans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.2 (2S,3S)-3-ethylaspartic acid
-
 Carboxydothermus hydrogenoformans ?
-
 ?
4.3.1.2 ethylfumarate + NH3
-
 Carboxydothermus hydrogenoformans ?
-
 ?
4.3.1.2 fumarate + NH3
-
 Carboxydothermus hydrogenoformans ?
-
 ?
4.3.1.2 mesaconate + ethylamine
-
 Carboxydothermus hydrogenoformans ?
-
 ?
4.3.1.2 mesaconate + hydrazine
-
 Carboxydothermus hydrogenoformans ?
-
 ?
4.3.1.2 mesaconate + hydroxylamine
-
 Carboxydothermus hydrogenoformans ?
-
 ?
4.3.1.2 mesaconate + methoxylamine
-
 Carboxydothermus hydrogenoformans ?
-
 ?
4.3.1.2 mesaconate + methylamine
-
 Carboxydothermus hydrogenoformans ?
-
 ?
4.3.1.2 mesaconate + NH3
-
 Carboxydothermus hydrogenoformans ?
-
 ?
4.3.1.2 methylfumarate + NH3
-
 Carboxydothermus hydrogenoformans ?
-
 ?
4.3.1.2 propylfumarate + NH3
-
 Carboxydothermus hydrogenoformans ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.1.2 dimer 2 * 49000 Carboxydothermus hydrogenoformans

Synonyms

EC Number Synonyms Comment Organism
4.3.1.2 Ch-MAL
-
 Carboxydothermus hydrogenoformans
4.3.1.2 methylaspartate ammonia lyase
-
 Carboxydothermus hydrogenoformans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.3.1.2 70
-
-
 Carboxydothermus hydrogenoformans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.3.1.2 50
-
 stable for more than 4 hours Carboxydothermus hydrogenoformans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.3.1.2 16
-
 (2S,3S)-3-ethylaspartic acid pH 9, 30°C Carboxydothermus hydrogenoformans
4.3.1.2 78
-
 (2S,3S)-3-ethylaspartic acid pH 9, 70°C Carboxydothermus hydrogenoformans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.2 9
-
-
 Carboxydothermus hydrogenoformans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.3.1.2 4.9
-
 (2S,3S)-3-ethylaspartic acid pH 9, 30°C Carboxydothermus hydrogenoformans
4.3.1.2 35
-
 (2S,3S)-3-ethylaspartic acid pH 9, 70°C Carboxydothermus hydrogenoformans