BRENDA - Enzyme Database show

Synthesis of green note aroma compounds by biotransformation of fatty acids using yeast cells coexpressing lipoxygenase and hydroperoxide lyase

Buchhaupt, M.; Guder, J.C.; Etschmann, M.M.; Schrader, J.; Appl. Microbiol. Biotechnol. 93, 159-168 (2012)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.92
gene HPLCl, overexpression in Saccharomyces cerevisiae strain BY4743 isogenic
Citrullus lanatus
Engineering
EC Number
Amino acid exchange
Commentary
Organism
4.2.1.92
additional information
development of a yeast-based whole cell biocatalyst able to transform polyunsaturated fatty acids into green note aldehydes and alcohols, evaluation, overview
Citrullus lanatus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.92
13-hydroperoxylinoleic acid
Citrullus lanatus
-
(Z)-3-hexanal + 12-oxododecenoic acid
-
-
?
4.2.1.92
alpha-linolenic acid
Citrullus lanatus
cleavage of 9-hydroperoxy fatty acid
(E,Z)-3,6-nonadienal + 9-oxononanoic acid
-
-
?
4.2.1.92
linoleic acid
Citrullus lanatus
cleavage of 9-hydroperoxy fatty acid
(Z)-3-nonenal + 9-oxononanoic acid
-
-
?
4.2.1.92
additional information
Citrullus lanatus
cleavage of 9-hydroperoxy fatty acids or 13-hydroperoxy fatty acids by a hydroperoxide lyase results in formation of aldehydes (Z)-3-nonenal/(E,Z)-3,6-nonadienal or (Z)-3-hexanal, and omega-oxo fatty acid 9-oxo-nonanoic acid or 12-oxo-dodecenoic acid, respectively
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.92
Citrullus lanatus
-
var. lanatus, gene HPLCl
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.92
13-hydroperoxylinoleic acid
-
729055
Citrullus lanatus
(Z)-3-hexanal + 12-oxododecenoic acid
-
-
-
?
4.2.1.92
alpha-linolenic acid
cleavage of 9-hydroperoxy fatty acid
729055
Citrullus lanatus
(E,Z)-3,6-nonadienal + 9-oxononanoic acid
-
-
-
?
4.2.1.92
linoleic acid
cleavage of 9-hydroperoxy fatty acid
729055
Citrullus lanatus
(Z)-3-nonenal + 9-oxononanoic acid
-
-
-
?
4.2.1.92
additional information
cleavage of 9-hydroperoxy fatty acids or 13-hydroperoxy fatty acids by a hydroperoxide lyase results in formation of aldehydes (Z)-3-nonenal/(E,Z)-3,6-nonadienal or (Z)-3-hexanal, and omega-oxo fatty acid 9-oxo-nonanoic acid or 12-oxo-dodecenoic acid, respectively
729055
Citrullus lanatus
?
-
-
-
-
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
4.2.1.92
25
-
assay at
Citrullus lanatus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.92
6.8
-
-
Citrullus lanatus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.92
gene HPLCl, overexpression in Saccharomyces cerevisiae strain BY4743 isogenic
Citrullus lanatus
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
4.2.1.92
additional information
development of a yeast-based whole cell biocatalyst able to transform polyunsaturated fatty acids into green note aldehydes and alcohols, evaluation, overview
Citrullus lanatus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.92
13-hydroperoxylinoleic acid
Citrullus lanatus
-
(Z)-3-hexanal + 12-oxododecenoic acid
-
-
?
4.2.1.92
alpha-linolenic acid
Citrullus lanatus
cleavage of 9-hydroperoxy fatty acid
(E,Z)-3,6-nonadienal + 9-oxononanoic acid
-
-
?
4.2.1.92
linoleic acid
Citrullus lanatus
cleavage of 9-hydroperoxy fatty acid
(Z)-3-nonenal + 9-oxononanoic acid
-
-
?
4.2.1.92
additional information
Citrullus lanatus
cleavage of 9-hydroperoxy fatty acids or 13-hydroperoxy fatty acids by a hydroperoxide lyase results in formation of aldehydes (Z)-3-nonenal/(E,Z)-3,6-nonadienal or (Z)-3-hexanal, and omega-oxo fatty acid 9-oxo-nonanoic acid or 12-oxo-dodecenoic acid, respectively
?
-
-
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.92
13-hydroperoxylinoleic acid
-
729055
Citrullus lanatus
(Z)-3-hexanal + 12-oxododecenoic acid
-
-
-
?
4.2.1.92
alpha-linolenic acid
cleavage of 9-hydroperoxy fatty acid
729055
Citrullus lanatus
(E,Z)-3,6-nonadienal + 9-oxononanoic acid
-
-
-
?
4.2.1.92
linoleic acid
cleavage of 9-hydroperoxy fatty acid
729055
Citrullus lanatus
(Z)-3-nonenal + 9-oxononanoic acid
-
-
-
?
4.2.1.92
additional information
cleavage of 9-hydroperoxy fatty acids or 13-hydroperoxy fatty acids by a hydroperoxide lyase results in formation of aldehydes (Z)-3-nonenal/(E,Z)-3,6-nonadienal or (Z)-3-hexanal, and omega-oxo fatty acid 9-oxo-nonanoic acid or 12-oxo-dodecenoic acid, respectively
729055
Citrullus lanatus
?
-
-
-
-
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
4.2.1.92
25
-
assay at
Citrullus lanatus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.92
6.8
-
-
Citrullus lanatus
General Information
EC Number
General Information
Commentary
Organism
4.2.1.92
physiological function
the enzyme is involved in synthesis of green note substances from linoleic acid and alpha-linolenic acid in the plant
Citrullus lanatus
General Information (protein specific)
EC Number
General Information
Commentary
Organism
4.2.1.92
physiological function
the enzyme is involved in synthesis of green note substances from linoleic acid and alpha-linolenic acid in the plant
Citrullus lanatus