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Literature summary extracted from

  • Miki, Y.; Asano, Y.
    Construction of the biosynthetic pathway for the cyanide-free production of phenylacetonitrile in Escherichia coli utilizing plant cytochrome P450 79A2 and bacterial aldoxime dehydratase (2014), Appl. Environ. Microbiol., 80, 6828-6836.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.14.14.40 synthesis biosynthetic pathway for the production of phenylacetonitrile in Escherichia coli utilizing enzymes from the plant glucosinolate-biosynthetic and bacterial aldoxime-nitrile pathways, i.e. introducing the genes encoding cytochrome P450 (CYP) 79A2 and CYP reductase from Arabidopsis thaliana, yielding the E,Z-phenylacetaldoxime-producing transformant and further introducing the aldoxime dehydratase (Oxd) gene from Bacillus sp. strain OxB-1, yielding the phenylacetonitrile-producing transformant. Expression of active CYP79A2 and concentration of biomass are improved by the combination of the autoinduction method, coexpression of groE, encoding the heat shock protein GroEL/GroES, N-terminal truncation of CYP79A2, and optimization of the culture conditions, yielding a more than 60fold concentration of E,Z-phenylacetaldoxime up to 2.9 mM Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.8.1.4 expressed in Escherichia coli C41 (DE3) cells Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.8.1.4 (Z)-phenylacetaldehyde oxime Bacillus sp. (in: Bacteria)
-
 phenylacetonitrile + H2O
-
 ?
4.8.1.4 (Z)-phenylacetaldehyde oxime Bacillus sp. (in: Bacteria) OxB-1
-
 phenylacetonitrile + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.40 Arabidopsis thaliana Q9FLC8
-
-
4.8.1.4 Bacillus sp. (in: Bacteria) P82604
-
-
4.8.1.4 Bacillus sp. (in: Bacteria) OxB-1 P82604
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.8.1.4 (Z)-phenylacetaldehyde oxime
-
 Bacillus sp. (in: Bacteria) phenylacetonitrile + H2O
-
 ?
4.8.1.4 (Z)-phenylacetaldehyde oxime
-
 Bacillus sp. (in: Bacteria) OxB-1 phenylacetonitrile + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.40 CYP79A2
-
 Arabidopsis thaliana
4.8.1.4 aldoxime dehydratase
-
 Bacillus sp. (in: Bacteria)
4.8.1.4 OXD
-
 Bacillus sp. (in: Bacteria)