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Literature summary extracted from

  • Isotani, K.; Kurokawa, J.; Suzuki, F.; Nomoto, S.; Negishi, T.; Matsuda, M.; Itoh, N.
    Gene cloning and characterization of two NADH-dependent 3-quinuclidinone reductases from Microbacterium luteolum JCM 9174 (2012), Appl. Environ. Microbiol., 79, 1378-1384.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.B52 pharmacology stereospecific production of (R)-3-quinuclidinol, an important chiral building block for the synthesis of various pharmaceuticals Microbacterium luteolum
1.1.1.B52 synthesis stereospecific production of (R)-3-quinuclidinol, an important chiral building block for the synthesis of various pharmaceuticals Microbacterium luteolum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.B52 expression in Escherichia coli. The gene is fused with the His6 tag and thrombin recognition sequence at the N-terminus under the control of the T7 promoter, 3-quinuclidinone reductase BacC Microbacterium luteolum
1.1.1.B52 expression in Escherichia coli. The gene is fused with the His6 tag and thrombin recognition sequence at the N-terminus under the control of the T7 promoter, 3-quinuclidinone reductase QNR Microbacterium luteolum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.B52 0.02
-
 NADH pH 7.0, 25°C, 3-quinuclidinone reductase QNR Microbacterium luteolum
1.1.1.B52 0.03
-
 NADH pH 7.0, 25°C, 3-quinuclidinone reductase BacC Microbacterium luteolum
1.1.1.B52 6.5
-
 3-quinuclidinone pH 7.0, 25°C, 3-quinuclidinone reductase QNR Microbacterium luteolum
1.1.1.B52 13.8
-
 3-quinuclidinone pH 7.0, 25°C, 3-quinuclidinone reductase BacC Microbacterium luteolum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.B52 30600
-
 3 * 30600, SDS-PAGE, 3-quinuclidinone reductase QNR Microbacterium luteolum
1.1.1.B52 32600
-
 2 * 32600, SDS-PAGE, 3-quinuclidinone reductase BacC Microbacterium luteolum
1.1.1.B52 72000
-
 gel filtration, 3-quinuclidinone reductase BacC Microbacterium luteolum
1.1.1.B52 94000
-
 gel filtration, 3-quinuclidinone reductase QNR Microbacterium luteolum

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.B52 Microbacterium luteolum L8B2H6
-
-
1.1.1.B52 Microbacterium luteolum JCM 9174 L8B2H6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.B52
-
 Microbacterium luteolum
1.1.1.B52 3-quinuclidinone reductase QNR Microbacterium luteolum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.B52 0.5
-
 pH 7.0, 25°C, 3-quinuclidinone reductase BacC Microbacterium luteolum
1.1.1.B52 8.4
-
 pH 7.0, 25°C Microbacterium luteolum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.B52 2-acetylpyridine + NADH + H+ activity is 16.8% compared to the activity with 3-quinuclidinone, 3-quinuclidinone reductase BacC Microbacterium luteolum ?
-
 ?
1.1.1.B52 2-acetylpyridine + NADH + H+ activity is 16.8% compared to the activity with 3-quinuclidinone, 3-quinuclidinone reductase BacC Microbacterium luteolum JCM 9174 ?
-
 ?
1.1.1.B52 3-quinuclidinone + NADH + H+ 3-quinuclidinone (5% w/v, 313 mM) is reduced to (R)-3-quinuclidinol with a molar conversion yield of 100% by 3-quinuclidinone reductase QNR. The optical purity is above 99.9%. No activity with NADPH. The enzyme is strictly specific for 3-quinuclidinone and shows no activity toward several ketones, including tropinone Microbacterium luteolum (R)-3-quinuclidinol + NAD+
-
 ?
1.1.1.B52 3-quinuclidinone + NADH + H+ 3-quinuclidinone (5% w/v, 313 mM) is reduced to (R)-3-quinuclidinol with a molar conversion yield of 94% bei 3-quinuclidinone reductase BacC. The optical purity is above 99.9%. No activity with NADPH Microbacterium luteolum (R)-3-quinuclidinol + NAD+
-
 ?
1.1.1.B52 3-quinuclidinone + NADH + H+ 3-quinuclidinone (5% w/v, 313 mM) is reduced to (R)-3-quinuclidinol with a molar conversion yield of 100% by 3-quinuclidinone reductase QNR. The optical purity is above 99.9%. No activity with NADPH. The enzyme is strictly specific for 3-quinuclidinone and shows no activity toward several ketones, including tropinone Microbacterium luteolum JCM 9174 (R)-3-quinuclidinol + NAD+
-
 ?
1.1.1.B52 3-quinuclidinone + NADH + H+ 3-quinuclidinone (5% w/v, 313 mM) is reduced to (R)-3-quinuclidinol with a molar conversion yield of 94% bei 3-quinuclidinone reductase BacC. The optical purity is above 99.9%. No activity with NADPH Microbacterium luteolum JCM 9174 (R)-3-quinuclidinol + NAD+
-
 ?
1.1.1.B52 7-oxabicyclo[4.1.0]heptan-2-one + NADH + H+ activity is 27.8% compared to the activity with 3-quinuclidinone, 3-quinuclidinone reductase BacC Microbacterium luteolum ?
-
 ?
1.1.1.B52 7-oxabicyclo[4.1.0]heptan-2-one + NADH + H+ activity is 27.8% compared to the activity with 3-quinuclidinone, 3-quinuclidinone reductase BacC Microbacterium luteolum JCM 9174 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.B52 dimer 2 * 32600, SDS-PAGE, 3-quinuclidinone reductase BacC Microbacterium luteolum
1.1.1.B52 trimer 3 * 30600, SDS-PAGE, 3-quinuclidinone reductase QNR Microbacterium luteolum

Synonyms

EC Number Synonyms Comment Organism
1.1.1.B52 BacC bacC isolated from Microbacterium luteolum shows 99.9% identity (100% amino acid identity) with the putative oxidoreductase gene bacC of the bacilysin biosynthetic gene cluster derived from Bacillus subtilis, suggesting that bacC is horizontally transferred from Bacillus species Microbacterium luteolum
1.1.1.B52 BacC
-
 Microbacterium luteolum
1.1.1.B52 QNR
-
 Microbacterium luteolum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.B52 25
-
 assay at Microbacterium luteolum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.B52 7 8 3-quinuclidinone reductase QNR Microbacterium luteolum
1.1.1.B52 7
-
 3-quinuclidinone reductase BacC Microbacterium luteolum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.B52 NADH no activity with NADPH, 3-quinuclidinone reductase QNR Microbacterium luteolum
1.1.1.B52 NADH no activity with NADPH, 3-quinuclidinone reductase BacC Microbacterium luteolum