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Literature summary extracted from

  • Yuan, P.; Meng, K.; Wang, Y.; Luo, H.; Shi, P.; Huang, H.; Tu, T.; Yang, P.; Yao, B.
    A low-temperature-active alkaline pectate lyase from Xanthomonas campestris ACCC 10048 with high activity over a wide pH range (2012), Appl. Biochem. Biotechnol., 168, 1489-1500.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.2 DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain JM109 Xanthomonas campestris

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.2 Cr3+ at 1 mM Xanthomonas campestris
4.2.2.2 Cu2+ at 1 mM Xanthomonas campestris
4.2.2.2 EDTA at 1 mM Xanthomonas campestris
4.2.2.2 Ni2+ at 1 mM Xanthomonas campestris
4.2.2.2 SDS at 1 mM Xanthomonas campestris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.2 Ca2+ required, best at 0.05 mM Xanthomonas campestris
4.2.2.2 Co2+ 1.4fold at 1 mM Xanthomonas campestris
4.2.2.2 Mn2+ 1.7fold at 1 mM Xanthomonas campestris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.2 38000
-
x * 38000, recombinant His-tagged enzyme, SDS-PAGE Xanthomonas campestris

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.2 Xanthomonas campestris
-
-
-
4.2.2.2 Xanthomonas campestris ACCC 10048
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.2 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chrmatography to homogeneity Xanthomonas campestris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.2 citrus pectin P9311
-
Xanthomonas campestris ?
-
?
4.2.2.2 citrus pectin P9311
-
Xanthomonas campestris ACCC 10048 ?
-
?
4.2.2.2 citrus pectin P9436
-
Xanthomonas campestris ?
-
?
4.2.2.2 citrus pectin P9436
-
Xanthomonas campestris ACCC 10048 ?
-
?
4.2.2.2 citrus pectin P9561
-
Xanthomonas campestris ?
-
?
4.2.2.2 citrus pectin P9561
-
Xanthomonas campestris ACCC 10048 ?
-
?
4.2.2.2 additional information the enzyme activity against polygalacturonic acid as 100%, r-PL D exhibits 91.7%, 47.3%, and 6.5% of the activity when pectin is methyl-esterified 34%, 70%, and 85%, respectively Xanthomonas campestris ?
-
?
4.2.2.2 additional information the enzyme activity against polygalacturonic acid as 100%, r-PL D exhibits 91.7%, 47.3%, and 6.5% of the activity when pectin is methyl-esterified 34%, 70%, and 85%, respectively Xanthomonas campestris ACCC 10048 ?
-
?
4.2.2.2 polygalacturonic acid
-
Xanthomonas campestris ?
-
?
4.2.2.2 polygalacturonic acid
-
Xanthomonas campestris ACCC 10048 ?
-
?

Subunits

EC Number Subunits Comment Organism
4.2.2.2 ? x * 38000, recombinant His-tagged enzyme, SDS-PAGE Xanthomonas campestris

Synonyms

EC Number Synonyms Comment Organism
4.2.2.2 alkaline pectate lyase
-
Xanthomonas campestris
4.2.2.2 PL D
-
Xanthomonas campestris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.2 30
-
recombinant His-tagged enzyme Xanthomonas campestris

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.2.2
-
30 a low-temperature-active alkaline pectate lyase, 35% of maximal activity at 0°C, maximal activity at 30°C Xanthomonas campestris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.2 9
-
recombinant His-tagged enzyme Xanthomonas campestris

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.2 3 12 over 45% of maximal activity within this range Xanthomonas campestris

General Information

EC Number General Information Comment Organism
4.2.2.2 evolution the enzyme belongs to family PF09492 Xanthomonas campestris
4.2.2.2 additional information the enzyme from Xanthomonas campestris ACCC 10048 is a low-temperature-active alkaline pectate lyase Xanthomonas campestris