Literature summary extracted from
Chhetri, D.R.; Gupta, S.; Mukherjee, A.K.; Adhikari, J.
L-myo-inositol-1-phosphate synthase expressed in developing organ: isolation and characterisation of the enzyme from human fetal liver (2012), Appl. Biochem. Biotechnol., 167, 2269-2282.
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
5.5.1.4 |
1.15 |
- |
2-deoxy-D-glucose 6-phosphate |
pH 7, 37°C, Vmax: 280 nM |
Homo sapiens |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
5.5.1.4 |
Ca2+ |
moderate activation |
Homo sapiens |
|
5.5.1.4 |
Cu2+ |
inhibition |
Homo sapiens |
|
5.5.1.4 |
Hg2+ |
inhibition |
Homo sapiens |
|
5.5.1.4 |
K+ |
moderate activation |
Homo sapiens |
|
5.5.1.4 |
NH4+ |
strong activation |
Homo sapiens |
|
5.5.1.4 |
Zn2+ |
inhibition |
Homo sapiens |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
5.5.1.4 |
170000 |
- |
amino acid sequence |
Homo sapiens |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
5.5.1.4 |
Homo sapiens |
Q9NPH2 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
5.5.1.4 |
enzyme is purified 51fold using ammonium sulfate fractionation followed by successive chromatography through DEAE cellulose and BioGel A columns |
Homo sapiens |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
5.5.1.4 |
liver |
fetal liver |
Homo sapiens |
- |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
5.5.1.4 |
-20°C, BioGel A purified fractions (final purification fraction), 5-7 days |
Homo sapiens |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
5.5.1.4 |
2-deoxy-D-glucose 6-phosphate |
- |
Homo sapiens |
5-deoxy-L-myo-inositol 1-phosphate |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
5.5.1.4 |
L-myo-Inositol-1-phosphate synthase |
- |
Homo sapiens |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
5.5.1.4 |
40 |
- |
- |
Homo sapiens |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
5.5.1.4 |
7 |
7.5 |
optimal activity |
Homo sapiens |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
5.5.1.4 |
NAD+ |
- |
Homo sapiens |
|