Literature summary extracted from

Gall, M.; Thomsen, M.; Peters, C.; Pavlidis, I.V.; Jonczyk, P.; Gruenert, P.P.; Beutel, S.; Scheper, T.; Gross, E.; Backes, M.; Geissler, T.; Ley, J.P.; Hilmer, J.M.; Krammer, G.; Palm, G.J.; Hinrichs, W.; Bornscheuer, U.T.
Enzymatic conversion of flavonoids using bacterial chalcone isomerase and enoate reductase (2014), Angew. Chem. Int. Ed. Engl., 53, 1439-1442.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.3.1.31	expressed in Escherichia coli Rosetta (DE3) cells	Eubacterium ramulus
5.5.1.6	expressed in Escherichia coli	Eubacterium ramulus

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.3.1.31	70000	-	x * 70000, SDS-PAGE	Eubacterium ramulus
1.3.1.31	75000	-	-	Eubacterium ramulus
5.5.1.6	32500	-	SDS-PAGE	Eubacterium ramulus

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1.3.1.31	chalcone + NAD+	Eubacterium ramulus	-	dihydrochalcone + NADH + H+	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.3.1.31	Eubacterium ramulus	V9P074	-	-
5.5.1.6	Eubacterium ramulus	V9P0A9	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.3.1.31	chalcone + NAD+	-	Eubacterium ramulus	dihydrochalcone + NADH + H+	-	?
1.3.1.31	additional information	the recombinant enzyme exhibits low activity for the conversion of the naringenin into phloretin	Eubacterium ramulus	?	-	?
5.5.1.6	eriodictyol	-	Eubacterium ramulus	eriodictyol chalcone	-	?
5.5.1.6	homoeriodictyol	-	Eubacterium ramulus	homoeriodictyol chalcone	-	?
5.5.1.6	naringenin	-	Eubacterium ramulus	naringenin chalcone	-	?
5.5.1.6	phloretin	-	Eubacterium ramulus	3-(4-hydroxyphenyl)propanoic acid	-	?

Subunits

EC Number	Subunits	Comment	Organism
1.3.1.31	?	x * 70000, SDS-PAGE	Eubacterium ramulus
1.3.1.31	?	x * 75000, calculated from amino acid sequence	Eubacterium ramulus
5.5.1.6	hexamer	6 * 32500, trimers of three dimer units as determined by crystal analysis	Eubacterium ramulus

Synonyms

EC Number	Synonyms	Comment	Organism
1.3.1.31	enoate reductase	-	Eubacterium ramulus
1.3.1.31	ERED	-	Eubacterium ramulus
5.5.1.6	Chalcone isomerase	-	Eubacterium ramulus
5.5.1.6	CHI	-	Eubacterium ramulus

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
5.5.1.6	45	-	-	Eubacterium ramulus

Temperature Stability [°C]

EC Number	Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
5.5.1.6	41	-	recombinant protein maintains 50% of its activity after incubation at 41°C for 6 h	Eubacterium ramulus

pH Range

EC Number	pH Minimum	pH Maximum	Comment	Organism
5.5.1.6	6.4	7.6	90% activity	Eubacterium ramulus

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.3.1.31	FAD	-	Eubacterium ramulus
1.3.1.31	NAD+	-	Eubacterium ramulus

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Release 2023.1 (January 2023)